Simultaneous determinations of plasma oxytocin and serum placental leucine aminopeptidase (P-LAP) during late pregnancy

Mizutani, Shigehiko; H, Hayakawa; Akiyama, Haruyuki; Sakura, Harutake; Yoshino, Masataka; Oya, Masakazu; Kawashima, Y

doi:10.1016/s0009-9120(82)90582-3

Cited by 35 publications

(28 citation statements)

References 12 publications

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“…In the early period, the high level of estrogen and progesterone may contribute to this pathophysiological phenomenon. During the late period, the OT level in plasma was elevated [9,10] . In the present study, when administrated simultaneously, the inhibitory effects of P and OT on gastrointestinal were strengthened each other.…”

Section: Liu Cy Et Al P and Gl Motility 339mentioning

confidence: 99%

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Effects of progesterone on gastric emptying and intestinal transit in male rats

Liu¹,

Chen²,

Liu³

et al. 2002

WJG

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AIM:To study the dose-dependent of progesterone (P) effect and the interaction between the oxytocin (OT) and P on gastrointestinal motility. METHODS:In order to monitor the gastric emptying and intestinal transit, the SD male rats were intubated via a catheter with normal saline (3ml/kg) containing Na 2 51 CrO 4 (0.5µ µ µ µ µCi/ml) and 10% charcoal. OT was dissolved into normal saline and P was dissolved into 75% alcohol.RESULTS: Low does of P (1mg/kg, i.p.) enhanced the gastric emptying (75±3%, P<0.05) and high dose of P (5mg/kg, i.p.) inhibit it (42±11.2%, P<0.01). P (1mg/kg) increased the intestinal transit (4.2±0.3, P<0.05) while the higher dose (10-20mg/kg) had no effect. OT (0.8mg/kg, i.p.) inhibited the gastric emptying (23.5± 9.8%, P<0.01). The inhibitory effects of P(20mg/kg) (32±9.7%, P<0.05) and OT (0.8mg/kg) on gastric emptying enhanced each other when the two chemicals were administrated simultaneously (17±9.4%, P<0.01).CONCLUSION: Low dose of P increased GI motility while high dose of P decreased it. During the later period of pregnancy, elevated plasma level of OT may also participate in the gastrointestinal inhibition.Liu CY, Chen LB, Liu PY, Xie DP, Wang PS. Effects of progesterone on gastric emptying and intestinal transit in male rats. World J Gastroenterol 2002;8(2):338-341

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Section: Liu Cy Et Al P and Gl Motility 339mentioning

confidence: 99%

“…The effects of E were testified by other scholars while the inhibitory effect of P was observed [8] . Oxytocin (OT) is also a hormone related to pregnancy, and its plasma concentration is higher during the late phase [9,10] . It is reported that OT inhibited gastric motility and secretion [11 -13] .…”

Section: Introductionmentioning

confidence: 99%

Effects of progesterone on gastric emptying and intestinal transit in male rats

Liu¹,

Chen²,

Liu³

et al. 2002

WJG

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“…The mean serum P-LAP levels during the last 28 days of pregnancy showed that P-LAP reached a high 11 days before the onset of labor and did not increase thereafter [43]. Labor may be initiated by an increase in oxytocin release together with a decrease in oxytocin degradation by oxytocinase.…”

Section: A Mechanisms Of Onset Of Labor and Transient Diabetes Insipmentioning

confidence: 94%

“…On the other hand, there was a significant increase in maternal plasma oxytocin with advancing gestation, although pronounced fluctuations in its levels in pregnant women is known [43]. Therefore, the maintenance of gestation despite the increased presence of oxytocin receptors in the uterus, is difficult.…”

Section: A Mechanisms Of Onset Of Labor and Transient Diabetes Insipmentioning

confidence: 99%

Physiological Role of Placental Proteases: Interaction Between Pregnancy-Induced Bioactive Peptides and Proteases

et al. 1994

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“…and 500 mg sodium citrate in 160 ml of distilled water was added to I ml blood in a glass lube which was allowed to stand in an ice-cold water bath. Previously, we reported that the addition of the chemicals and the chilling of the blood prevented destruction of oxytocin by oxytocinasc [9]. The mixture was immediately cen trifuged at 4°C (3.000 g for 20 min), and the plasma was stored at -3 0°C for analysis.…”

Section: Immediately After Cesarean Section Of the Iugr Infants Umbimentioning

confidence: 99%

Possible Effects of Placental Leucine Aminopeptidase on the Regulation of Brain-Gut Hormones in the Fetoplacental Unit

Mizutani

Goto²,

Tsujimoto³

et al. 1996

Neonatology

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The hydrolysis of somatostatin by human placental subcellular fractions and pregnancy sera was studied in the presence of selective inhibitors and the antibody against pregnancy serum oxytocinase (placental leucine aminopeptidase; EC3.4.11.3) by measuring the released amino acids by high-performance liquid chromatography. We also studied the degradation of other brain-gut hormones, such as glucagon, growth hormone, growth hormone releasing factor, and insulin, in the human placenta and found that the human placenta degrades somatostatin, glucagon, and growth hormone releasing factor, but not insulin and growth hormone. The degradation velocity of somatostatin was ten times greater than that of growth hormone releasing factor in placental microsomal fractions. Our data suggest that the stimulatory control by growth hormone releasing factor is dominant in the fetal growth hormone secretion. Our data also identified the somatostatin-degrading protease in human placenta using placental leucine aminopeptidase. It is known that the mean somatostatin levels in the umbilical artery are about 2.5-fold higher than those in the umbilical vein. Our data on somatostatin levels in umbilical artery and vein of intrauterine growth retardation human fetuses showed that the ratio umbilical artery/vein is around 1. Since insulin is known to be the primary hormone regulating the ratio of fetal growth, our data suggest that the degradation of somatostatin in the placenta is decreased and that elongation of somatostatin effects may result in the inhibition of insulin secretion in the intrauterine growth retardation fetus.

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Simultaneous determinations of plasma oxytocin and serum placental leucine aminopeptidase (P-LAP) during late pregnancy

Cited by 35 publications

References 12 publications

Effects of progesterone on gastric emptying and intestinal transit in male rats

Effects of progesterone on gastric emptying and intestinal transit in male rats

Physiological Role of Placental Proteases: Interaction Between Pregnancy-Induced Bioactive Peptides and Proteases

Possible Effects of Placental Leucine Aminopeptidase on the Regulation of Brain-Gut Hormones in the Fetoplacental Unit

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