Simultaneous development of acute phase response and autoantibodies in preclinical rheumatoid arthritis

Nielen, Markus M J; Schaardenburg, D. van; Reesink, H. W.; Twisk, Jos W. R.; Stadt, R J van de; Horst‐Bruinsma, Irene E. van der; Koning, M H M T de; Habibuw, Moud R.; Dijkmans, Ben A. C.

doi:10.1136/ard.2005.040659

Cited by 119 publications

(90 citation statements)

References 6 publications

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“…It has been observed by others that high levels of cytokines (26) and systemic indicators of inflammation (34) develop together with autoantibodies in RA. In normal subjects who had B cells capable of producing anti-CCP in the current study, levels of inflammatory cytokines were not elevated.…”

Section: Discussionmentioning

confidence: 99%

Production of Autoantibodies against Citrullinated Antigens/Peptides by Human B Cells

Bellatin

Han

Fallena

et al. 2012

The Journal of Immunology

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Autoantibodies against citrullinated protein Ags (ACPA) are associated with the development of rheumatoid arthritis (RA). This immune response against citrullinated protein Ags, which is thought to be facilitated by certain MHC HLA-DR alleles, is highly specific for this disease and has been speculated to be involved in the pathogenesis. We have previously studied cultures of B cells for the production of Abs against HLA Ags. The aim of the current study was to examine the role of B cells in the production of ACPA in patients with RA. Peripheral blood B cells from RA patients and healthy people were cultured with EL4-B5, a murine cell line expressing human CD40L, and with T cell factors to stimulate the in vitro production of Abs by B cells isolated from peripheral blood. ACPA were produced by cultured B cells from RA patients, as determined by reactivity to cyclic citrullinated peptide (CCP). The results showed that 22% of the healthy persons tested also had B cells that could produce ACPA. Patients with HLA-DR alleles carrying the RA-associated shared epitope appeared to have more B cells with autoimmune potential for CCP than those without such HLA alleles (odds ratio 8.1, p = 0.001). In healthy individuals, anti-CCP–producing B cells were also observed more frequently if the RA-associated MHC genes were present (odds ratio 8.0, p = 0.01). Analysis of B cells in cultures may shed light on the interaction of genetic and environmental factors in the development of RA.

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Section: Discussionmentioning

confidence: 99%

Production of Autoantibodies against Citrullinated Antigens/Peptides by Human B Cells

Bellatin

Han

Fallena

et al. 2012

The Journal of Immunology

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“…CFA-induced arthritis is the most widely used chronic test model which is associated with an immune-mediated inflammatory reaction and rat is unique in developing polyarthritis after CFA treatment and hence they were used for the study [38]. In first phase, irritant nature of the adjuvant induces inflammation and hence measurement of 3 to 5 day effect of treatment represents the efficacy on inflammation of primary lesions such as edema and soft-tissue thickening.…”

Section: Discussionmentioning

confidence: 99%

Formulation and Evaluation of Novel Herbal Aerosol for Arthritis

Rajasekaran¹

2017

JRAD

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Arthritis is the most common cause of disability in adults all over the world, limiting everyday activities for billions of people. Herbs have been paid more attention since they have very little side effects. Cardiospermum halicacabum Linn, (Sapindaceae) and VitexnegundoLinn. (Verbenaceae) were used in traditional medicine for the treatment of arthritis. The present investigation was undertaken to evaluate anti-arthritic activity of novel herbal aerosol spray formulation containing methanol leaf extracts of Cardiospermum halicacabum (MLECH) and Vitexnegundo (MLEVN). A novel topical aerosol spray was formulated using MLECH and MLEVN(2% w/v each) and their quality control, stability and efficacy studies was carried out as per United States Pharmacopoeia (USP), International Conference on Harmonization (ICH) and Organization for Economic Co-Operation and Development (OECD) guidelines respectively. CFA induced arthritis method was employed for the evaluation of antiarthritic activity. Assessment of body weight, biochemical parameters, hematological parameters, serum biomarker levels, photographic, radiographic analysis and histopathological investigations were carried out as a supportive evidence for the anti-arthritic efficacy of the developed novel herbal aerosol spray formulation. The results of the quality control test complied with USP standards and the stability study clearly revealed that the formulated topical herbal aerosol spray was found to be stable. Significant (p<0.01) alterations in rat paw volume, body weight, biochemical, hematological, serum biomarker levels and reduction in inflammation and hyperplasia of synovium of formulation treated rats were observed compared with arthritic rats. Study concluded that the formulated novel herbal aerosol spray exhibited good anti-arthritic activity.

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“…FCA-induced polyarthritis is associated with an immune-mediated inflammatory reaction and the rat is unique in developing polyarthritis after FCA treatment (Nielen et al, 2006).…”

Section: Paw Volumementioning

confidence: 99%

Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model

Rajasekaran

Govindarjan

Arivukkarasu

2016

Braz. J. Pharm. Sci.

125

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The objective of the study is to formulate and evaluate a topical herbal gel containing Cardiospermum halicacabum and Vitex negundo leaf extracts for their anti-arthritic activity in rats. Twelve herbal gel formulations were prepared using 1.5% of gelling agents carbopol 934 (F1-F6) and carbopol 940 (F6-F12) and they were evaluated for physical appearance, net content, viscosity, extrudability, pH, spreadability, in vitro diffusion profile and primary skin irritation tests. The stability study for the topical herbal gel formulation was done as per ICH guidelines and anti-arthritic activity was evaluated by Freund's Complete Adjuvant (FCA) induced arthritis method. Assessment of body weight, paw volume, hematological and biochemical parameters, histopathological examination and In vitro determination of serum biomarkers were also carried out. Formulated gels were homogenous, stable and complied with the guidelines. Among the formulations, F4 showed better release (98.4 %) characteristics than other formulations. No erythema or edema was observed in the skin irritation test confirming the gel was nontoxic and safe. Topical application of the herbal gel F4 containing carbopol 934 displayed significant (p < 0.001) anti-arthritic activity compared to diseased rats. Reduction in paw volume, no agglutination in C -reactive protein and rheumatic factor, reduction in TNFα level, regaining of normal hematological, and biochemical parameters, reduction in spleen and thymus weight and histopathological examination supported the anti-arthritic activity of the gel formulation.Uniterms: Arthritis/treatment/herbal gel. Cardiospermum halicacabum/topical herbal gel. Vitex negundo/ topical herbal gel. Medicinal plants/arthritis.

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Simultaneous development of acute phase response and autoantibodies in preclinical rheumatoid arthritis

Abstract: Both the acute phase response and autoantibody formation often develop years before the first symptoms of RA occur, and these phenomena are probably closely connected in time.

Cited by 119 publications

References 6 publications

Production of Autoantibodies against Citrullinated Antigens/Peptides by Human B Cells

Production of Autoantibodies against Citrullinated Antigens/Peptides by Human B Cells

Formulation and Evaluation of Novel Herbal Aerosol for Arthritis

Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model

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