Simultaneous Distillation-Extraction, Hydrodistillation and Static Headspace Methods for the Analysis of Volatile Secondary Metabolites of<i>Alpinia zerumbet</i>(Pers.) Burtt<i>et</i>Smith. from Southeast Brazil

Victório, Cristiane Pimentel; Riehl, Carlos Alberto da Silva; Lage, Celso Luiz Salgueiro

doi:10.1080/0972060x.2009.10643703

Cited by 17 publications

(29 citation statements)

References 16 publications

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“…The main constituents were terpinen-4-ol, 1,8 cineole and γ-terpinene (Table 1, Figure 1) (Victório, 2008;Victório et al, 2009a). These constituents were found in studies on the essential oil from other samples of A. zerumbet (Zoghbi et al, 1999;Ali et al, 2002;Elzaawely et al, 2007;Victório et al, 2009b). Caryophyllene oxide was also found in low amounts (Figure 1).…”

Section: Resultsmentioning

confidence: 93%

Chemical composition of the fractions of leaf oil of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. and antimicrobial activity

Victório¹,

Alviano²,

Alviano³

et al. 2009

Rev. bras. farmacogn.

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M. Sm., extraído por hidrodestilação, foi analisado por CG/DIC e CG/EM a fim de se determinar sua composição e atividade antimicrobiana. O óleo apresentou um elevado teor de monoterpenos oxigenados (52,5%): terpinen-4-ol, 1,8-cineol e γ-terpineno como principais constituintes. A atividade antimicrobiana do óleo foi analisada pelos testes de difusão em agar e ensaios de bioautografia. Através do teste de difusão em agar, todas as bactérias e fungos testados foram inibidos pelo óleo essencial. Placas preparativas de CCD (cromatografia em camada fina) foram eluídas em hexano: acetato de etila (70:20, v/v), e o óleo foi separado em três frações que foram raspadas, extraídas da sílica e identificadas por CG/EM. A análise bioautográfica permitiu detectar pronunciada inibição de Cryptococcus neoformans pelas frações F2 e F3, ambas ricas em monoterpenos oxigenados. A fração F2 apresentou 1,8 cineol (9,6%), linalol (3,7%) e óxido de cariofileno (5,4%), enquanto a fração F3 mostrou principalmente terpinen-4-ol (43,6%) e cerca de 32,7% de sesquiterpenos oxigenados. Os dados obtidos indicam os componentes do óleo de A. zerumbet envolvidos na atividade antimicrobiana.Unitermos: óleo essencial, análise bioautográfica, Alpinia zerumbet, planta medicinal, Zingiberaceae. ABSTRACT:Leaf oil prepared by hydrodistillation of Alpinia zerumbet Pers.) B.L. Burtt & R.M. Sm. was analyzed by GC/FID and GC/MS to determine the major compounds and it was also evaluated for antimicrobial activity. The oil presented a high content of oxygenated monoterpenes (52.5%), terpinen-4-ol, 1,8 cineole and γ-terpinene as the major constituents. The antimicrobial activity of leaf oil was tested by drop diffusion and bioauthography methods. Through drop test, all bacteria and fungi tested were inhibited by leaf oil. Preparative TLC (thin-layer chromatography) plates were developed using the mobile phase hexane : ethyl acetate (70:20, v/v) and fractions of the leaf oil were separated in three zones, scraped, extracted from silica and identified by GC/MS. The bioauthography method permitted to verify pronounced inhibition of Cryptococcus neoformans by fractions F2 and F3, both rich in oxygenated monoterpenes. The fraction F2 comprised 1,8 cineole (9.6%), linalool (3.7%) and caryophyllene oxide (5.4%), while fraction F3 showed mainly terpinen-4-ol (43.6%) and an amount of 32.7% oxygenated sesquiterpenes. The obtained data may be used to suggest the constituents of A. zerumbet leaf oil involved in antimicrobial activity.

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Section: Resultsmentioning

confidence: 93%

Chemical composition of the fractions of leaf oil of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. and antimicrobial activity

Victório¹,

Alviano²,

Alviano³

et al. 2009

Rev. bras. farmacogn.

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“…Mpalantinos et al (1998) suggested that flavonoids and kavapyrones are responsible for vasodilator activity of aqueous extracts of A. zerumbet, while Lahlou et al (2003) attributed it to some essential oil constituents therapeutic effects of A. zerumbet. A similarity between Alpinia purpurata and A. zerumbet was found in phytochemical studies, in terms of essential oils and flavonoids composition (Victório, 2008;Victório et al, 2009b). The presence of flavonoids supported evidence for the chemosystematic features of the Zingiberiflorae, Zingiberaceae family (Pugialli et al, 1993;Victório et al, 2009b).…”

Section: Introductionmentioning

confidence: 77%

“…Alpinia zerumbet, known as 'colônia', is widely used in northeastern and southeastern Brazil as infusions or decoctions for treatment of arterial hypertension. Hypotensive effects of this species have been justified by composition of hydroalcoholic and aqueous extracts and essential oils Victório et al, 2009a). According to Mendonça et al (1991) a decrease blood pressure in rats and dogs was observed using hydroalcoholic extracts of A. zerumbet.…”

Section: Introductionmentioning

confidence: 99%

Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro

Victório

Kuster

Moura

et al. 2009

Braz. J. Pharm. Sci.

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Nowadays, the high blood pressure is one of the main causes of death and cardiovascular diseases. Vasodilator drugs are frequently used to treat arterial hypertension. Experiments were undertaken to determine whether hydroalcoholic extracts obtained from leaves of field-grown Alpinia purpurata and A. zerumbet cultured in vitro under different plant growth regulators induce a vasodilator effect on Wistar rat mesenteric vascular bed pre-contracted with norepinephrine. Plant extracts were able to induce a long-lasting endothelium-dependent vasodilation. Efficiency on activity of A. purpurata reached 87% at concentration of 60 mg. The extract of A. zerumbet maintained in medium containing IAA, induced the relaxation (17.4%) at 90 μg, as compared to the control (MS0) that showed a better vasodilator effect (60%). These results are in agreement with the quantification of phenolic compounds in the extracts, which were 50% lower for those plants cultured in IAA. A. purpurata was assayed for the first time in relation to its vasodilator activity. This paper showed a strong probability of correlation between the pharmacological activities of A. purpurata with their content in phenolic compounds. Atualmente, a hipertensão arterial é uma das maiores causas de morte e de doenças cardiovasculares. Os vasodilatadores são freqüentemente utilizados no tratamento da hipertensão. Extratos hidroalcoólicos de Alpinia purpurata de campo e de A. zerumbet cultivada in vitro sob diferentes reguladores de crescimento vegetal foram ensaiados no leito mesentérico de ratos Wistar. Os extratos de A. purpurata e A. zerumbet produziram efeito vasodilatador com padrão de resposta dose-dependente de duração prolongada. Extratos da espécie A. purpurata tiveram efeito vasodilatador de 87% na dose de 60 μg. O extrato obtido de folhas de A. zerumbet oriundas das culturas mantidas em meio contendo AIA (ácido indol acético) inibiu o relaxamento (17,4%) na dose de 90 μg em relação ao controle (MS0), com o qual foi verificado melhor efeito vasodilatador (60%). Estes resultados estão de acordo com a concentração de fenóis totais que foi 50% menor para os extratos de plantas cultivadas in vitro em AIA. A espécie A. purpurata foi pela primeira vez ensaiada quanto à atividade vasodilatadora. Os resultados obtidos indicaram a presença de substâncias fenólicas provavelmente correlacionadas à ação terapêutica de A. purpurata.

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“…In the essential oil of A. purpurata, α-pinene, β-pinene and 1,8 cineol predominate in leaves and flowers. Sesquiterpenes β-caryophyllene and caryophyllene oxide are also common among this species (Zoghbi et al, 1999;Joseph et al, 2001;Mallavarapu et al, 2002;Victório et al, 2009e;2009f;2010b).…”

Section: Phytochemical Studiesmentioning

confidence: 99%

Therapeutic value of the genus Alpinia, Zingiberaceae

Victório¹

2011

Rev. bras. farmacogn.

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Plants containing bioactive substances have increasingly become the object of research studies, particularly those plants with therapeutic value. Many species of the genus Alpinia provide a variety of medicinal properties, such as, Alpinia zerumbet (Pers.) Burtt et Smith and A. purpurata (Vieill) K. Schum, which have a significant presence in Brazil. These species have been commercialized in the food and cosmetic industries. However, their greatest importance arises from the medicinal properties of their essential oils containing flavonoids, terpenoids and kavalactones which have been used in folk medicine to treat, for example, arterial hypertension and inflammatory processes. In addition, such species are also used in multidisciplinary studies, including phytochemistry, ethnobotany and biology, indicating the key pharmacological role of this genus in everyday life. Therefore, this work aims to present a bibliographic review of the genus Alpinia and its significance in therapeutic applications.

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Simultaneous Distillation-Extraction, Hydrodistillation and Static Headspace Methods for the Analysis of Volatile Secondary Metabolites ofAlpinia zerumbet(Pers.) BurttetSmith. from Southeast Brazil

Cited by 17 publications

References 16 publications

Chemical composition of the fractions of leaf oil of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. and antimicrobial activity

Chemical composition of the fractions of leaf oil of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. and antimicrobial activity

Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro

Therapeutic value of the genus Alpinia, Zingiberaceae

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