Simultaneous electrochemical detection of uric acid and ascorbic acid at a poly(N,N-dimethylaniline) film-coated GC electrode

Roy, Protiva Rani; Okajima, Takaharu; Ohsaka, Takeo

doi:10.1016/j.jelechem.2003.07.003

Cited by 128 publications

(59 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a contrast on the correctness of the results, the UA concentrations of the same samples were analyzed simultaneously by the HPLC method. The results summarized in Table 1 show that the UA concentrations found in urine samples are fairly close to those reported elsewhere, [19][20][21][22][23] and the results of HPLC. 9 …”

Section: Analysis Of Urine Samplessupporting

confidence: 87%

See 1 more Smart Citation

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

et al. 2009

View full text Add to dashboard Cite

A uric acid biosensor based on the direct electron transfer of a hemoglobin-encapsulated chitosan-modified glassy carbon electrode was developed for a highly sensitive and selective analysis in urine samples. The modified electrode was prepared by the encapsulation of hemoglobin and uricase in a chitosan matrix. The hydrogen peroxide produced from the catalytic oxidation of uric acid by uricase was reduced electrocatalytically by immobilized hemoglobin and used to obtain a sensitive amperometric response to uric acid. The linear response of the uric acid concentrations ranged from 2.00 to 30.0 µM with a correlation of 0.9982, the detection limit of uric acid was estimated to be 0.85 µM at a signal/noise ratio of 3. The uric acid biosensor can efficiently exclude the interference of commonly coexisted ascorbic acid, dopamine, epinephrine, etc. The relative standard deviation was under 2.56% (n = 5) for the determination of real samples. This biosensor is satisfactory for the determination of human urine samples compared with the HPLC-UV method.

show abstract

Section: Analysis Of Urine Samplessupporting

confidence: 87%

“…[20][21][22] However, the interferences contributed by other electroactive species, such as ascorbic acid (AA), epinephrine (EP) and dopamine (DA) often disturb the proper determination of UA. Also, the reported amperometric biosensor can only achieve a relative high detection limit of about 2.0 µM.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

et al. 2009

View full text Add to dashboard Cite

show abstract

“…pH 7.2 was used for determination of ascorbic acid since it reflects real sample conditions and most of the studies reported in the literature are carried out in this pH. [32][33][34] The oxidation current was found to increase with increasing the concentration of ascorbic acid; Fig. 5 shows the voltammetric responses of the MnO2-CP-E composite electrodes to the additions of ascorbic acid into a pH 7.2 phosphate buffer solution.…”

Section: Ascorbic Acid Detectionmentioning

confidence: 99%

Manganese Dioxide Graphite Composite Electrodes: Application to the Electroanalysis of Hydrogen Peroxide, Ascorbic Acid and Nitrite

et al. 2007

View full text Add to dashboard Cite

“…The GC electrode is fouled by adsorption of oxidized AA [29][30][31]. Rueda et al [32] studied the oxidation of AA on a gold electrode over a wide pH range and proposed that AA is oxidized to dehydroascorbic acid (DHAA) via the radical anion intermediate monodehydroascorbic acid.…”

Section: Voltamogram Reproducibilitymentioning

confidence: 99%

Voltammetric determination of dopamine in the presence of ascorbic and uric acids using partial least squares regression: determination of dopamine in human urine and plasma

2009

View full text Add to dashboard Cite

Abstract:A new differential pulse voltammetric method for dopamine determination at a bare glassy carbon electrode has been developed. Dopamine, ascorbic acid (AA) and uric acid (UA) usually coexist in physiological samples. Because AA and UA can be oxidized at potentials close to that of DA it is difficult to determine dopamine electrochemically, although resolution can be achieved using modified electrodes. Additionally, oxidized dopamine mediates AA oxidation and the electrode surface can be easily fouled by the AA oxidation product. In this work a chemometrics strategy, partial least squares (PLS) regression, has been applied to determine dopamine in the presence of AA and UA without electrode modification. The method is based on the electrooxidation of dopamine at a glassy carbon electrode in pH 7 phosphate buffer. The dopamine calibration curve was linear over the range of 1 -313 µM and the limit of detection was 0.25 µM. The relative standard error (RSE %) was 5.28%. The method has been successfully applied to the measurement of dopamine in human plasma and urine.© Versita Warsaw and Springer-Verlag Berlin Heidelberg.

show abstract

Simultaneous electrochemical detection of uric acid and ascorbic acid at a poly(N,N-dimethylaniline) film-coated GC electrode

Cited by 128 publications

References 41 publications

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

Manganese Dioxide Graphite Composite Electrodes: Application to the Electroanalysis of Hydrogen Peroxide, Ascorbic Acid and Nitrite

Voltammetric determination of dopamine in the presence of ascorbic and uric acids using partial least squares regression: determination of dopamine in human urine and plasma

Contact Info

Product

Resources

About