Simultaneous enhancement on renewable bioactive compounds from Porphyridium cruentum via a novel two-stage cultivation

Huang, Zicheng; Chen, Zhong; Dai, Jingxuan; Li, Shuning; Zheng, Mingmin; He, Yongjin; Wang, Mingzi; Chen, Bilian

doi:10.1016/j.algal.2021.102270

Cited by 16 publications

(6 citation statements)

References 45 publications

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“…ARA, C16:0, EPA, and C18:2ω6 were found to be the main fatty acids in P. cruentum CCALA 415, which is consistent with the findings in most previous reports [3,[28][29][30][31]. However, there were some differences in other fatty acids with low contents.…”

Section: Discussionsupporting

confidence: 91%

“…Cohen et al detected C18:4ω3 and C20:3ω3 in P. cruentum [30]. Huang et al identified C14:0, C20:1, and C20:2ω6, but not C18:3ω6, in P. purpureum [31]. These differences might be related to Porphyridium species, culture conditions, and fatty acid determination methods.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the contents of ARA and EPA were 1.14-2.46% DW and 0.49-1.67% DW, respectively (Figure 3). Huang et al reported that the contents of ARA and EPA in P. purpureum were 1.18% DW and 1.84% DW, respectively [31], whereas Jiao et al reported that the contents were 1.5% DW and 0.7% DW, respectively [32]. In P. cruentum CCALA 415, all the fatty acids in the ω6 fatty acid pathway were detected, but two fatty acids (C18:4ω3 and C20:4ω3) in the ω3 fatty acid pathway were not detected (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

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Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics

Li,

Wang

et al. 2024

Marine Drugs

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The marine red microalga Porphyridium can simultaneously synthesize long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (C20:5, EPA) and arachidonic acid (C20:4, ARA). However, the distribution and synthesis pathways of EPA and ARA in Porphyridium are not clearly understood. In this study, Porphyridium cruentum CCALA 415 was cultured in nitrogen-replete and nitrogen-limited conditions. Fatty acid content determination, transcriptomic, and lipidomic analyses were used to investigate the synthesis of ARA and EPA. The results show that membrane lipids were the main components of lipids, while storage lipids were present in a small proportion in CCALA 415. Nitrogen limitation enhanced the synthesis of storage lipids and ω6 fatty acids while inhibiting the synthesis of membrane lipids and ω3 fatty acids. A total of 217 glycerolipid molecular species were identified, and the most abundant species included monogalactosyldiglyceride (C16:0/C20:5) (MGDG) and phosphatidylcholine (C16:0/C20:4) (PC). ARA was mainly distributed in PC, and EPA was mainly distributed in MGDG. Among all the fatty acid desaturases (FADs), the expressions of Δ5FAD, Δ6FAD, Δ9FAD, and Δ12FAD were up-regulated, whereas those of Δ15FAD and Δ17FAD were down-regulated. Based on these results, only a small proportion of EPA was synthesized through the ω3 pathway, while the majority of EPA was synthesized through the ω6 pathway. ARA synthesized in the ER was likely shuttled into the chloroplast by DAG and was converted into EPA by Δ17FAD.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics

Li,

Wang

et al. 2024

Marine Drugs

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“…The primary source of industrial-grade PE are strains from the red algae Porphyridium sp., especially P. cruentum [ 18 , 28 , 29 , 30 , 31 , 32 ] and P. purpureum [ 17 , 18 , 19 , 33 , 34 ]. However, this genus is also known as a primary source of sulfated EPS; therefore, the available protocols for extracting PE are adapted to the unique requirements of these unicellular red algae, which makes the extraction and recovery process tedious and time-consuming [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Vergel-Suarez

García-Martínez

López-Barrera

et al. 2023

BioTech

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Drying the biomass produced is one of the critical steps to avoid cell degradation; however, its high energy cost is a significant technological barrier to improving this type of bioprocess’s technical and economic feasibility. This work explores the impact of the biomass drying method of a strain of Potamosiphon sp. on the extraction efficiency of a phycoerythrin-rich protein extract. To achieve the above, the effect of time (12–24 h), temperature (40–70 °C), and drying method (convection oven and dehydrator) were determined using an I-best design with a response surface. According to the statistical results, the factors that most influence the extraction and purity of phycoerythrin are temperature and moisture removal by dehydration. The latter demonstrates that gentle drying of the biomass allows removing the most significant amount of moisture from the biomass without affecting the concentration or quality of temperature-sensitive proteins.

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“…Pigments were quantified by recording absorbance at 650 nm (allophycocyanin), 620 nm (phycocyanin), and 565 nm (phycoerythrin). Quantitative calculation was made based on equations using specific absorption coefficients [32].…”

Section: Phycobiliproteins Contentmentioning

confidence: 99%

Effects of Silver Nanoparticles on the Red Microalga Porphyridium purpureum CNMN-AR-02, Cultivated on Two Nutrient Media

Rudi,

Cepoi,

Chiriac

et al. 2024

Marine Drugs

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The purpose of this study was to examine the influence of 10 and 20 nm nanoparticles (AgNPs) on the growth and biochemical composition of microalga Porphyridium purpureum CNMN-AR-02 in two media which differ by the total amount of mineral salts (MM1 with 33.02 g/L and MM2 with 21.65 g/L). Spectrophotometric methods were used to estimate the amount of biomass and its biochemical composition. This study provides evidence of both stimulatory and inhibitory effects of AgNPs on different parameters depending on the concentration, size, and composition of the nutrient medium. In relation to the mineral medium, AgNPs exhibited various effects on the content of proteins (an increase up to 20.5% in MM2 and a decrease up to 36.8% in MM1), carbohydrates (a decrease up to 35.8% in MM1 and 39.6% in MM2), phycobiliproteins (an increase up to 15.7% in MM2 and 56.8% in MM1), lipids (an increase up to 197% in MM1 and no changes found in MM2), antioxidant activity (a decrease in both media). The composition of the cultivation medium has been revealed as one of the factors influencing the involvement of nanoparticles in the biosynthetic activity of microalgae.

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Simultaneous enhancement on renewable bioactive compounds from Porphyridium cruentum via a novel two-stage cultivation

Cited by 16 publications

References 45 publications

Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics

Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics

Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Effects of Silver Nanoparticles on the Red Microalga Porphyridium purpureum CNMN-AR-02, Cultivated on Two Nutrient Media

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