Simultaneous estimation of gabapentin and methylcobalamin in bulk and pharmaceutical dosage form by RP-HPLC

Abstract

A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Gabapentin & Methylcobalamin from bulk and formulation. Chromatographic separation was achieved Isocratically on an Inertsil C18 column (150x4.6, 5µ particle size) using a mobile phase Buffer: Acetonitrile in the ratio of 60:40 v/v. The flow rate was 1.0 ml/min, effluents were detected at 264 nm and 10µl of sample was injected. Retention time of Gabapentin & Methylcobalamin was found to be 2.7 and 4.13 min respectively. Linearity of the method was in the concentration range of 25-150 µg for Gabapentin & 0.125-0.750 µg for Methylcobalamin. Percent recoveries obtained for both the drugs were 100.00%. The percentage RSD for precision of the method was found to be less than 2%. The method was validated according to the ICH guidelines. The method developed was successfully applied for the analysis of simultaneous estimation of Gabapentin & Methylcobalamin tablets and was fairly good in comparison with other methods.

Stability indicating RP‐HPLC method for methylcobalamin determination in different dosage forms: Application to photodegradation kinetics and pH rate profiling

“…A spectrofluorimetric method studies the effect of pH on the kinetics of aerobic photolysis of MeCbl [13]; however, this study did not consider MeCbl hydrolysis under acidic and alkaline conditions due to methyl‐cobalt (CoIII) bond hydrolysis. Also, several stability indicating methods to study MeCbl stability in the presence of other drugs such as dosulepin [14, 15], gabapentin [16], epalrestat, and pregabalin [17] were reported.…”

Application of Molecularly Imprinted Poly-Itaconic/Multiwalled Carbon Nanotubes for Selective and Sensitive Electrochemical Detection of Linagliptin

“…Several methods of analysis were reported for LNG detection in literature including Liquid chromatography, [2][3][4] RP-HPLC, [5][6][7][8] HPLC, 9,10 LC-MS/MS 11,12 and HPTLC 13 and Spectrophotometry. [14][15][16][17] Recently, two voltammetric electrochemical methods have been reported for LNG, 18,19 the first is based on studying the oxidative behavior of LNG on unmodified pencil graphite electrode, while the second reported the use of cobalt oxide nanoparticles and multi-walled carbon nanotubes modified carbon paste electrodes with LOD of 2.1 × 10 −7 and 1.1 × 10 −5 M, respectively.…”