Simultaneous estimation of parameters governing the vertical and horizontal transfer of antibiotic resistance genes

Mishra, Sulagna; Klümper, Uli; Voolaid, Veiko; Berendonk, Thomas U.; Kneis, David

doi:10.1016/j.scitotenv.2021.149174

Cited by 16 publications

(8 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spread of antibiotic resistance is significantly aided by both [44]. Since the optrA gene was discovered in China in 2015, it has been widely spread and detected in bacterial genera with diverse origins, including Enterococcus and Staphylococcus, in many different nations [45].…”

Section: Discussionmentioning

confidence: 99%

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Han

Wan

et al. 2023

Antibiotics

View full text Add to dashboard Cite

This study was performed to investigate the presence and characteristics of the oxazolidinone resistance genes optrA and cfr(D) in Streptococcus parasuis. In total, 36 Streptococcus isolates (30 Streptococcus suis isolates, 6 Streptococcus parasuis isolates) were collected from pig farms in China in 2020–2021, using PCR to determine the presence of optrA and cfr. Then, 2 of the 36 Streptococcus isolates were further processed as follows. Whole-genome sequencing and de novo assembly were employed to analyze the genetic environment of the optrA and cfr(D) genes. Conjugation and inverse PCR were employed to verify the transferability of optrA and cfr(D). The optrA and cfr(D) genes were identified in two S. parasuis strains named SS17 and SS20, respectively. The optrA of the two isolates was located on chromosomes invariably associated with the araC gene and Tn554, which carry the resistance genes erm(A) and ant(9). The two plasmids that carry cfr(D), pSS17 (7550 bp) and pSS20-1 (7550 bp) have 100% nucleotide sequence identity. The cfr(D) was flanked by GMP synthase and IS1202. The findings of this study extend the current knowledge of the genetic background of optrA and cfr(D) and indicate that Tn554 and IS1202 may play an important role in the transmission of optrA and cfr(D), respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Han

Wan

et al. 2023

Antibiotics

View full text Add to dashboard Cite

show abstract

“…To confirm if observed results are exclusive to the above donor-recipient combination or to the pKJK5 plasmid, experiments were repeated using Escherichia coli MG1655:: lacI q -pLpp-mCherry-Km R ( 43 ) as the donor strain of IncP-1β broad-host range plasmid pB10 ( 47 , 48 ) that confers resistance to amoxicillin, tetracycline, sulfonamides, and streptomycin. Conditions were exactly as described above with the lone exception that the incubation temperature was increased to 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…The latter equation captures the dynamics of recipient ( R ), donor ( D ), and transconjugant ( T ) populations in liquid cultures due to the simultaneous effects of growth and conjugation ( 49 , 50 ). Such models can readily be adapted to account for the impact of various environmental conditions on plasmid transfer efficiencies ( 48 ). Here, we amend the equations of Levin et al ( 49 ) with an empirical term reflecting the effect of MWCNT on the rate constant of plasmid transfer.…”

Section: Methodsmentioning

confidence: 99%

Multiwalled Carbon Nanotubes Promote Bacterial Conjugative Plasmid Transfer

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Horizontal gene transfer (HGT) is the main mechanism underlying the enrichment and spread of ARGs in the environment ( Debroas and Siguret, 2019 ; Lerminiaux and Cameron, 2019 ). ARGs can be horizontally transferred in three ways: conjugation mediated by mobile genetic elements, transformation by cell-free DNA, and phage-mediated transduction ( Brown-Jaque et al, 2015 ; Mishra et al, 2021 ). Among the three HGT mechanisms, conjugation is generally considered the predominant mode of ARG dissemination in environmental settings ( von Wintersdorff et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Bacteriophages: Underestimated vehicles of antibiotic resistance genes in the soil

et al. 2022

View full text Add to dashboard Cite

Bacteriophages (phages), the most abundant biological entities on Earth, have a significant effect on the composition and dynamics of microbial communities, biogeochemical cycles of global ecosystems, and bacterial evolution. A variety of antibiotic resistance genes (ARGs) have been identified in phage genomes in different soil samples. Phages can mediate the transfer of ARGs between bacteria via transduction. Recent studies have suggested that anthropogenic activities promote phage-mediated horizontal gene transfer events. Therefore, the role of phages in the dissemination of ARGs, which are a potential threat to human health, may be underestimated. However, the contribution of phages to the transfer of ARGs is still poorly understood. Considering the growing and wide concerns of antibiotic resistance, phages should be considered a research focus in the mobile resistome. This review aimed to provide an overview of phages as vehicles of ARGs in soil. Here, we summarized the current knowledge on the diversity and abundance of ARGs in soilborne phages and analyzed the contribution of phages to the horizontal transfer of ARGs. Finally, research deficiencies and future perspectives were discussed. This study provides a reference for preventing and controlling ARG pollution in agricultural systems.

show abstract

Simultaneous estimation of parameters governing the vertical and horizontal transfer of antibiotic resistance genes

Cited by 16 publications

References 52 publications

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Multiwalled Carbon Nanotubes Promote Bacterial Conjugative Plasmid Transfer

Bacteriophages: Underestimated vehicles of antibiotic resistance genes in the soil

Contact Info

Product

Resources

About