Simultaneous extraction and quantification of albendazole and triclabendazole using vortex‐assisted hollow‐fiber liquid‐phase microextraction combined with high‐performance liquid chromatography

A sensitive method for determining sulfonamides in water was developed and validated through in situ derivatization and hollow-fiber liquid-phase microextraction with ultra-high performance liquid chromatography and fluorescence detection. The target sulfonamides were sulfadiazine, sulfacetamide, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfachloropyridazine, sulfamethoxazole, and sulfisoxazole. Following in situ derivatization with fluorescamine, three-phase hollow-fiber liquid-phase microextraction with an S 6/2 polypropylene hollow-fiber membrane was applied automatically using a multipurpose autosampler. Experimental parameters including derivatization time, choice of organic phase, pH of donor and acceptor phase, stirring rate, extraction temperature and time were optimized. Under optimized conditions, the target sulfonamides achieved excellent linearity with correlation coefficients of 0.9924-0.9994 within the concentration range of 0.05-5 μg/L. The limits of detection of the eight sulfonamides were 3.1-11.2 ng/L, and the limits of quantification were 10.3-37.3 ng/L. Enrichment factors of 0.1 and 5 μg/L sulfonamides spiked in lake water were 14-60, and recoveries were 56-113% with relative standard derivations of 3-19%. Applied with the developed method, sulfamerazine and sulfamethoxazole were measurable in both influent and effluent water of the three sewage treatment plants in Guangzhou, China. The developed method was sensitive and provided an alternative method for simultaneously enriching and quantifying multiple sulfonamides in environmental water.

Section: Resultsmentioning

confidence: 99%

In situ derivatization and hollow‐fiber liquid‐phase microextraction to determine sulfonamides in water using UHPLC with fluorescence detection

Yang

Shi

Li³

et al. 2018

“…The enhancement factor (EF) and extraction recovery (ER) of the developed microextraction method were calculated by the following equations:

EF = \frac{m_{a}}{m_{w}}

ER % = EF \times (\frac{V_{organic}}{V_{aqueous}}) \times 100

where m a , m w , V organic , and V aqueous are the slope of calibration graph after extraction of OTA by the developed method, the slope of calibration graph without extraction of OTA, the volume of the organic extractant, and the volume of the aqueous sample, respectively .…”

Section: Methodsmentioning

confidence: 99%

“…LPME as a miniaturized application of LLE has rapid, low cost, and simple procedure. LPME has several modes including single drop microextraction , hollow fiber LPME , dispersive liquid‐phase microextraction (DLPME) , and solidified floating organic drop (SFOD) microextraction .…”

Section: Introductionmentioning

confidence: 99%

Syringe‐to‐syringe dispersive liquid‐phase microextraction solidified floating organic drop combined with high‐performance liquid chromatography for the separation and quantification of ochratoxin A in food samples

Asadi¹

2017

Self Cite

A new, simple, and rapid syringe-to-syringe dispersive liquid-phase microextraction with solidified floating organic drop was used for the separation and preconcentration of ochratoxin A from grain and juice samples before its quantification using high-performance liquid chromatography and fluorescence detection. Factors influencing the microextraction efficiency of ochratoxin A, such as sample solution pH, type and volume of organic extractant, salt concentration, number of injections, and volume of the sample, were studied and optimized. Under the optimum properties, the calibration graph showed linearity in the range of 65.0-700.0 ng/L (coefficient of determination = 0.9991). The limit of detection was 20.0 ng/L. The inter-day and intra-day relative standard deviations were in the range of 5.0-8.5%. This method was successfully applied for the quantification of ochratoxin A in grain and juice samples.

“…In HF‐LPME method, analytes are extracted from aqueous donor phase into organic phase by an organic solvent immobilized in the porous wall of hollow fiber . Large molecules cannot permeate through the pores of hollow fiber wall, thereby allows the HF‐LPME method to have such excellent clean‐up efficiency . In addition, its other advantages, including high enrichment factor, required negligible volume of solvents, and low cost, also promote this method to be a promising and reliable extraction technology in the sample preparation field .…”

Section: Introductionmentioning

confidence: 99%

Matrix solid‐phase dispersion coupled with hollow fiber liquid phase microextraction for determination of triazine herbicides in peanuts

Piao

Jiang

et al. 2019

Two extraction procedures, matrix solid‐phase dispersion and hollow fiber liquid‐phase microextraction, were combined and applied to determine triazine herbicides in peanut samples. The results showed that the established method has high extraction efficiency and could greatly eliminate the interferences from complex matrix samples. A series of important experimental parameters were all investigated in detail. Under the optimal conditions, the developed method has the limits of detection for triazine herbicides in the range of 0.05 to 1.71 μg/kg. Moreover, it has the recovery in the range of 80.4–120.0% with relative standard deviations of equal or lower than 8.9%. The established method may have a great potential in separation, enrichment, and purification of triazines from complex fatty solid samples.