Simultaneous Fluorescence and Chemiluminescence Turned on by Aggregation-Induced Emission for Real-Time Monitoring of Endogenous Superoxide Anion in Live Cells

Niu, Jinye; Fan, Jilin; Xu, Wei; Xiao, Yongsheng; Xie, Xilei; Jiao, Xiaoyun; Sun, Chuanzhi; Tang, Bo

doi:10.1021/acs.analchem.7b01425

Cited by 86 publications

(59 citation statements)

References 35 publications

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“…Utilizing imidazopyrazinone as a recognition group, a dual sensing platform with simultaneous turn‐on signals of fluorescence (FL) and CL triggered by O 2 .− was presented by our group . The structure of probe 14 contains two segments, the fluorescence moiety is a tetraphenylethene group with excellent aggregation‐induced emission (AIE) properties, and the CL moiety is an imidazopyrazinone with good CL response to O 2 .− (Figure ).…”

Section: Current Status Of Fluorescent Probes For O2−mentioning

confidence: 99%

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

et al. 2019

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The superoxide anion (O2.−) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.− in related diseases, developing effective methods for monitoring O2.− in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal‐spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.− inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.−‐responsive fluorescent probes. Moreover, we present the challenges for detecting O2.− and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.− as well as O2.−‐associated active molecules in living cells and in vivo.

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Section: Current Status Of Fluorescent Probes For O2−mentioning

confidence: 99%

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

et al. 2019

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“…For example, ROS/RNS are closely related to various biological processes, but excess levels can cause oxidative/nitrosative stress and cell and tissue damage, which manifest as neurodegenerative disorders, inflammation, and cancer . AIE probes have proven to be powerful tools for detecting chemical species in vivo, including hydrogen peroxide (H 2 O 2 ), superoxide anion (O 2 •− ), singlet oxygen ( 1 O 2 ), hypochlorous acid/hypochlorite (HClO/ClO − ), hydrogen sulfide (H 2 S), and sulfur dioxide (SO 2 ) …”

Section: Disease Diagnosismentioning

confidence: 99%

Promising Applications of AIEgens in Animal Models

Situ

Zhao

et al. 2019

Small Methods

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organisms cannot be achieved from these static findings. Therefore, gaining in-depth insights into biological and physiological functions at the in vivo level is of great significance, and various imaging techniques have been explored for this purpose. [1] In addition to the clinically available magnetic resonance imaging (MRI), ultrasonic imaging, computed tomography (CT), positron emission tomography (PET), and single-photon emission computed tomography, fluorescence imaging (another distinct type of optical imaging [1c] from bioluminescence imaging [2] ) has attracted increasing attention, owing to its advantages of high temporal-spatial resolution and sensitivity, noninvasive, and real-time applications, and the ability to provide dynamic information for basic research and (pre-)clinical settings at cellular and subcellular levels. [3] Fluorescent probes or contrast agents are essential for fluorescence imaging. Compared with fluorescent proteins, [4] inorganic nanoparticles (NPs), [5] such as quantum dots, [6] upconversion NPs, [7] and metallic NPs, [8] and organic dyes [9] (e.g., U.S. Food and Drug Administration (FDA)-approved 5-ALA, indocyanine green (ICG), and methylene blue), organic NPs [10] have obvious advantages, such as easy preparation and good biocompatibility. However, many organic NPs show weak fluorescence because they are made with conventional organic dyes, which suffer from the aggregation-caused quenching (ACQ) effect, i.e., their fluorescence is partially or completely quenched in the aggregated state.The advent of the aggregation-induced emission (AIE) concept offers a new strategy to deal with ACQ. [11] AIE refers to the interesting photophysical phenomenon that luminogens are weakly fluorescent or nonfluorescent in solution but show greatly enhanced emission in the aggregated state, primarily because of restricted intramolecular motion. [12] The past 19 years have witnessed spectacular advances of AIEgens, [13] due to their advantages of structural variety, easy functionalization, strong luminescence, large Stokes shift, high photobleaching-resistance, and so on. In particular, various AIE bioprobes have been designed: [14] (i) water-soluble AIEgens, which are comprised of a hydrophobic AIE core and hydrophilic units (e.g., ionic groups, peptides, carbohydrates, DNA, aptamers, antibodies, and poly(ethylene glycol) (PEG)); (ii) bare AIEgen dots (nanoaggregates); iii) AIEgen/biopolymer dots, which can be obtained by loading AIEgens onto biopolymer matrixes covalently (e.g., chitosan, dextran, and starch) or noncovalently (e.g., bovine serum albumin, human serum albumin (HSA), and fetal bovine serum (FBS)); (iv)In vivo investigations using small animal models are of great significance for the comprehensive understanding of biological and physiological functions-seeing is believing. Fluorescence imaging can provide real-time and dynamic information of living systems. During the past few years, aggregation-induced emission luminogens (AIEgens) are widely and rapidly developed for biolog...

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“…The insights obtained from the advances in aggregation‐induced emission (AIE) materials inspired us to develop a distinct CL sensor that boasts the ultrahigh concentrated CL units in one nanoparticle as shown in Scheme . Where, the synthesized tetraphenylethylene (TPE)‐phthalhydrazid (PH) (TPE‐PH) can aggregate to the highly fluorescent nanoparticle (NTPE‐PH) in aqueous.…”

Section: Introductionmentioning

confidence: 99%

Real‐Time Mapping of Ultratrace Singlet Oxygen in Rat during Acute and Chronic Inflammations via a Chemiluminescent Nanosensor

Zhang

Hongbo

et al. 2019

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Sensing nonradiation‐induced singlet oxygen (1O2) in whole‐animal is deemed as one of the most challenging tasks in noninvasive techniques due to the µs level lifetime of 1O2 and quenching by numerous reductants in tissues. Here a distinct chemiluminescent (CL) nanosensor (NTPE‐PH) that boasts ultrahigh concentrated CL units in one nanoparticle is reported. Taking advantage of the intramolecular energy transfer mechanism that promises high energy transfer efficiency and the aggregation‐induced emission behavior that guarantees high CL amplification, the NTPE‐PH sensor is sensitive to a nm level 1O2. Experiments demonstrate that the NTPE‐PH yields a highly selective CL response toward 1O2 among common reactive oxygen species. With proved low cytotoxicity and good animal compatibility, real‐time mapping of ultratrace 1O2 in whole‐animal during acute and chronic inflammations is first achieved. It is anticipated that the NTPE‐PH sensor can be a useful tool for monitoring 1O2 variation during immune response and pathological processes corresponding to different stimuli, even with drug treatment included.

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Simultaneous Fluorescence and Chemiluminescence Turned on by Aggregation-Induced Emission for Real-Time Monitoring of Endogenous Superoxide Anion in Live Cells

Cited by 86 publications

References 35 publications

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Promising Applications of AIEgens in Animal Models

Real‐Time Mapping of Ultratrace Singlet Oxygen in Rat during Acute and Chronic Inflammations via a Chemiluminescent Nanosensor

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