Simultaneous Heterotrophic Nitrification and Aerobic Denitrification byChryseobacteriumsp. R31 Isolated from Abattoir Wastewater

Abstract

A heterotrophic carbon utilizing microbe (R31) capable of simultaneous nitrification and denitrification (SND) was isolated from wastewater of an Indian slaughterhouse. From an initial COD value of 583.0 mg/L, 95.54% was removed whilst, from a starting NH4 +-N concentration of 55.7 mg/L, 95.87% was removed after 48 h contact. The concentrations of the intermediates hydroxylamine, nitrite, and nitrate were low, thus ensuring nitrogen removal. Aerobic denitrification occurring during ammonium removal by R31 was confirmed by utilization of both nitrate and nitrite as nitrogen substrates. Glucose and succinate were superior while acetate and citrate were poor substrates for nitrogen removal. Molecular phylogenetic identification, supported by chemotaxonomic and physiological properties, assigned R31 as a close relative of Chryseobacterium haifense. The NH4 +-N utilization rate and growth of strain R31 were found to be higher at C/N = 10 in comparison to those achieved with C/N ratios of 5 and 20. Monod kinetic coefficients, half saturation concentration (K s), maximum rate of substrate utilization (k), yield coefficient, (Y) and endogenous decay coefficient (K d) indicated potential application of R31 in large-scale SND process. This is the first report on concomitant carbon oxidation, nitrification, and denitrification in the genus Chryseobacterium and the associated kinetic coefficients.

The abundance and diversity of heterotrophic bacteria as a function of harvesting frequency of duckweed (Lemna minor L.) in recirculating aquaculture systems

“…The Bacillus consisted of obligate aerobes ( B. subtilis ), or facultative anaerobes ( B. licheniformis) , which are known to have a major role in heterotrophic nitrification (Kundu et al . ). Meanwhile, the Pseudomonas, the most abundant heterotrophic bacteria in marine RAS nitrification biofilters (Borges et al .…”

“…Members of the genera are abundant in freshwater and marine ecosystems and become dominant in response to the input of organic substrates (Kundu et al . ). Chrysobacterium spp.…”

Mathematical simulating the process of aerobic granular sludge treating high carbon and nitrogen concentration wastewater

“…With the increase of C/N in inflow, the AOB and nitrite oxidizing bacteria (NOB) significantly decrease, while the heterotrophic bacteria gradually increase [20,21]. In recent years, there are many reports about heterotrophic nitrification and aerobic denitrification [22][23][24]. Different from autotrophic nitrifiers, heterotrophicnitrifying bacteria can simultaneously using carbon and nitrogen pollutants.…”

“…Different from autotrophic nitrifiers, heterotrophicnitrifying bacteria can simultaneously using carbon and nitrogen pollutants. Most of them are also capable of aerobic denitrification, and can convert ammonia to gaseous nitrogen at the same time [24,25]. It indicates that heterotrophic nitrifiers are more suitable to treat high carbon and nitrogen wastewater under aerobic condition.…”

“…Meanwhile, the process of heterotrophic nitrification and aerobic denitrification has always been related to high C/N ratios [22,24]. Recently, aerobic process of treating high carbon and nitrogen wastewater (e.g.…”