Simultaneous HPLC Analysis of Three Flavonoids in the Extracts ofArtocarpus heterophyllusHeartwoods

Abstract

− A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of three antibacterial flavonoids, artocarpanone, artocarpin, and cycloartocarpin in ethyl acetate extracts from Artocarpus heterophyllus heartwoods. Separation was achieved using a TSK-gel ODS-80Tm column (5 µm, 4.6 × 150 mm) at 25 o C with a gradient elution system of methanol and water as follows: 0-8 min, 60:40; 8-27 min, 80:20; 27-35 min, 60:40, v/v, at a flow rate of 1 mL/min, and a quantitative UV detection at 285 nm. The method was validated by measuring the key parameters, including specificity, linearity, sensitivity, accuracy, repeatability and reproducibility. A high degree of specificity and sensitivity was achieved. The calibration curves for all three flavonoids showed good linearity with a coefficient of determinations (R 2 ) of ≥ 0.9995. The recoveries of the method were from 98-104%, with good reproducibility and repeatability (RSD values of less than 2%) were also achieved. Ethyl acetate was the best solvent for extraction of these three flavonoids using the heat reflux conditions for 1 h. This optimized sample preparation and HPLC method can be practically used for a routine standardization process of the extracts from the A. heterophyllus heartwoods.

Flavonoids of Artocarpus heterophyllus Lam. heartwood inhibit the innate immune responses of human phagocytes

“…The percentage inhibition of the extract and artocarpin on chemotaxis, ROS production and MPO activity were relatively similar. Our previous study demonstrated that artocarpin was the major compound in A. heterophyllus extract, while ethyl acetate was the suitable solvent for extraction of this compound …”

“…Our previous study demonstrated that artocarpin was the major compound in A. heterophyllus extract, while ethyl acetate was the suitable solvent for extraction of this compound. [23] Structure-activity analysis of the flavonoids on phagocytosis activity indicated that a saturation of the C-2 and C-3 in the C ring as well as 2 hydroxyl group in the B ring (C-2 0 and C-4 0 ) and a methoxy group in the A ring of artocarpanone might contribute towards its inhibitory activity, particularly on ROS production and chemotaxis activity of phagocytes. It has been shown that saturated C-2 and C-3 bond in the C ring, increasing number of -OH in the B ring, as well as one methoxy substitution in the A ring of flavonoid compound increased superoxide scavenging activity.…”

“…HPLC analysis of the ethyl acetate extract was performed according to the chromatographic condition described in our previous study . Briefly, HPLC analysis was carried out using the following condition; reverse‐phase column (TSK‐gel ODS‐80Tm column, 150 × 4.6 mm i.d.…”

“…[20,22] HPLC analysis of the ethyl acetate extract of Artocarpus heterophyllus heartwood HPLC analysis of the ethyl acetate extract was performed according to the chromatographic condition described in our previous study. [23] Briefly, HPLC analysis was carried out using the following condition; reverse-phase column (TSK-gel ODS-80Tm column, 150 - compounds was performed by comparing the retention times of the peaks in the extract and those of the standards. The amount of each compound in the extract was determined by plotting calibration curves of each standard solution with four different concentrations vs the areas under the peaks.…”

Dual impact of different drying treatments and ethanol/water ratios on antioxidant properties and colour attribute of jackfruit leaves (Artocarpus heterophyllus Lam.) Mastura variety (J35)

“…4.6 × 250 mm, 5 μm column). The analysis was performed according to Septama and Panichayupakaranant (2016) with modification of the adjusted mobile phase. A mobile phase was prepared that consisted of methanol and water (85% methanol and 15% deionized water), at a flow rate of 1 mL/min.…”