Simultaneous Identification and Quantification of Hydroquinone, Tretinoin and Betamethasone in Cosmetic Products by Isocratic Reversed Phase High Performance Liquid Chromatography

Abstract

Objective: The objective of this study was to obtain a simple and selective analysis method for determination of hydroquinone, tretinoin and betamethasone in whitening creams using reversed phase high-performance liquid chromatography (HPLC).
 Methods: Reverse Phase HPLC was used for method development, validation studies, and sample analysis. The method was optimized by evaluating several parameters that affects extraction of the sample, composition and types of mobile phase and also flow rate. Chromatographic separation was optimized on a C18 column [Sunfire, 250 x 4.6 mm, 5µm] utilizing a mobile phase consisting acetonitrile, methanol (90:10 v/v) and slightly addition of glacial acetic acid to reach pH 5in the ratio of 30: 50:20 v/v at a flow rate of 0.8 ml/min with UV detection at 270 nm and 350 nm.
 Results: The analytical methods fulfilled the validation requirements including accuracy, precision, linearity, selectivity, detection limits, and quantitation limits. The results showed the mean levels of hydroquinone, tretinoin and betamethasone in samples A and B were 1.78%; 0.07%; 0.12% and 2.00%; 0.07%; 0.13% respectively.
 Conclusion: The method was successfully applied for the determination of cosmetic formulation containing hydroquinone, tretinoin and betamethasone simultaneously. There were seven samples analyzed and two samples were positive containing hydroquinone, tretinoin, and betamethasone.

Cosmetic and Pharmaceutic Products with Selected Natural and Synthetic Substances for Melasma Treatment and Methods of Their Analysis

“…The literature of recent years has been dominated by studies on the simultaneous determination of several whitening ingredients present in a cosmetic or pharmaceutical product. They are mainly based on chromatographic techniques and concern the simultaneous detection of chemicals such as hydroquinone and retinoic acid [189], hydroquinone, hydrocortisone acetate, and tretinoin [190], hydroquinone, tretinoin, and betamethasone [191], arbutin, niacinamide, and 3-O-ethyl ascorbic acid [192], hydroquinone, resorcinol, catechol, and 3,3 -dichlorobenzidine [193], as well as two glucocorticoids, namely clobetasol 17-propionate and betamethasone 17-valerate [194], present in various forms of cosmetic and pharmaceutical products.…”

A highly efficient rGO grafted MoS2 nanocomposite for dye adsorption and electrochemical detection of hydroquinone in wastewater

“…Different techniques used for Hq detection include chromatography, spectrophotometry, electrochemistry and chemiluminescence. 3,4 Among the various techniques, enzymatic electrochemical biosensors based on the laccase (Lac) enzyme are the most reliable for detecting phenolic compounds. 5 Lac consists of multi-copper atoms that can perform oxido-reductase reactions by catalyzing phenolic compounds to their oxidized form and simultaneously reducing the molecular oxygen to water.…”