Simultaneous identification of three pseudoallergic components in Danshen injection by using high‐expression Mas‐related G protein coupled receptor X2 cell membrane chromatography coupled online to HPLC–ESI‐MS/MS

Abstract

Adverse drug reactions of Danshen injection mainly manifested as pseudoallergic reactions. In the present study, salvianolic acid A and a pair of geometric isomers (isosalvianolic acid C and salvianolic acid C) were identified as pseudoallergic components in Danshen injection by a high-expression Mas-related G protein coupled receptor X2 cell membrane chromatography coupled online with high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Their pseudoallergic activities were evaluated by in vitro assay, which were consistent with the retention times on the cell membrane chromatography column. Salvianolic acid C, the most outstanding compound, was further found to induce pseudoallergic reaction through Mas-related G protein coupled receptor X2. All the results above indicated that the system developed in this study is an effective method for simultaneously analyzing pseudoallergic components, even those with similar structures and the microcomponents in complex samples (salvianolic acid C in Danshen injection).

Modulation of the Mas-Related G Protein-Coupled Receptor X2 (MRGPRX2) by Xenobiotic Compounds and Its Relevance to Human Diseases

“…Exogenous ligands of MRGPRX2 include the cationic polymer compound 48/80 (C48/80), which is commonly used in receptor functional assays, and a variety of drugs approved by the Food and Drug Administration (FDA), such as fluoroquinolones (e.g., ciprofloxacin), neuromuscular blocking agents (e.g., rocuronium, atracurium), opioids (e.g., morphine), and many others [ 4 , 9 , 28 ]. MRGPRX2 can also be activated or inhibited by other exogenous agents, such as bacterial quorum sensing proteins, insect venoms [ 3 , 29 , 30 ], or many different plant xenobiotics ( Figure 1 ) [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]; representatives of which are described in the following part of this review.…”

Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices

“…The use of silica introduces silica groups in the process of studying ligand-protein interactions, which does not closely resemble physiological conditions. However, numerous drug discovery approaches utilizing CMCs have been reported in the literature, for example [40][41][42][43]. This review purely focuses on the approach with the artificial membrane as the support for the adsorbing cell membrane fragments and therefore CMC is not further discussed in this paper.…”

Establishment of angiotensin-converting enzyme 2 and cluster of differentiation 147 dual target cell membrane chromatography based on SNAP-tag technology for screening anti severe acute respiratory syndrome coronavirus 2 active components

“…As an affinity chromatography method, cell membrane chromatography (CMC) has been shown to be a powerful tool for screening target components in complex samples [ 15 , 16 ]. In our previous study, CMC methods based on related receptors were successfully used to screen for antiviral components [17] . However, more than one antiviral component may be present in complex samples and act on more than a single receptor [18] .…”