Simultaneous Light Emitting Diode-Induced Fluorescence and Contactless Conductivity Detection for Capillary Electrophoresis

Tan, Feng; Yang, Bingcheng; Guan, Yafeng

doi:10.2116/analsci.21.583

Cited by 39 publications

(34 citation statements)

References 13 publications

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“…Tan et al [36] reported on the simultaneous fluorescence and CC detection for determination of labeled amino acids and small proteins [36]. However, CC detectors are mostly hyphenated with UV detectors [37,38].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous contactless conductivity detection and UV detection for the study of separation of tamsulosin enantiomers in discontinuous electrolyte systems by CE

et al. 2006

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This work shows the potential of using discontinuous electrolyte systems for the separation of tamsulosin enantiomers by CE. Sulfated beta-cyclodextrin was used as a chiral selector. In acidic electrolytes, sulfated beta-cyclodextrin migrates as an anion and the analyte (tamsulosin) migrates as a cation. Due to this, four experimental arrangements were proposed. These arrangements differ in composition of electrolytes in the inlet compartment, in the capillary and in the outlet compartment. The separation of tamsulosin enantiomers in acetate buffers with sodium and Tris counterions was studied. Simultaneous contactless conductivity detection and UV detection were used for the study of the separation mechanism in these systems. Mobilities of sulfated beta-cyclodextrin were used for the calculation of the time when the analyte migrates through the BGE zone with the selector. The simulation program Simul 4.0 was used for the calculations of the concentration profiles of the electrolyte components dependent on the time of the separation. The mechanism of enantioseparation in these arrangements was suggested.

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Section: Introductionmentioning

confidence: 99%

Simultaneous contactless conductivity detection and UV detection for the study of separation of tamsulosin enantiomers in discontinuous electrolyte systems by CE

et al. 2006

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“…In this study, offline FITC-labeled AAs were separated and detected by the combined detector, the LODs were in the submicromolar level [14].…”

Section: Ce/fluorescencementioning

confidence: 96%

Recent advances in amino acid analysis by CE

et al. 2007

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This paper describes a number of articles that have been published on amino acid (AA) analysis using CE during the period from June 2005 to May 2007. This review article follows the format of the previous articles of Smith (Electrophoresis 1999, 20, 3078-3083), Prata et al.. (Electrophoresis 2001, 22, 4129-4138), and Poinsot et al.. (Electrophoresis 2003, 24, 4047-4062 and Electrophoresis 2006, 27, 176-194). Several new developments in AA analysis with CE are reported describing the use of laser-emitting diodes for LIF, MS, and chips. In addition, we describe articles concerning clinical studies and neuroclinical applications.

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“…Tan et al [36] reported a combined detection system using contactless conductometric detection and fluorescent detection for CE in a common cell. A blue LED was used for the excitation source, and an optical fiber was used to collect the fluorescence.…”

Section: Jong and Lucymentioning

confidence: 99%

CE detector based on light‐emitting diodes

et al. 2007

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CE detectors based on light-emitting diodes (LEDs) as light sources are receiving considerable attention due to their exceptionally high stability, high intensity, low cost, and a variety of wavelengths in the UV and visible spectrum. This article is a comprehensive review on CE methods using LED-based detectors with absorbance and fluorescence detection, and several applications on the determination of riboflavin, bacteria, drug, and amino acids in biological samples are described.

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Simultaneous Light Emitting Diode-Induced Fluorescence and Contactless Conductivity Detection for Capillary Electrophoresis

Cited by 39 publications

References 13 publications

Simultaneous contactless conductivity detection and UV detection for the study of separation of tamsulosin enantiomers in discontinuous electrolyte systems by CE

Simultaneous contactless conductivity detection and UV detection for the study of separation of tamsulosin enantiomers in discontinuous electrolyte systems by CE

Recent advances in amino acid analysis by CE

CE detector based on light‐emitting diodes

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