Simultaneous liquid chromatographic assessment of thiamine, thiamine monophosphate and thiamine diphosphate in human erythrocytes: a study on alcoholics

Mancinelli, Rosanna; Ceccanti, Mauro; Guiducci, Maria Soccorsa; Sasso, G F; Sebastiani, Gemma; Attilia, Maria Luisa; Allen, John P.

doi:10.1016/s1570-0232(03)00139-9

Cited by 76 publications

(72 citation statements)

References 24 publications

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“…aruplab.com) for assessment of TDP in whole blood. Approximately 80% of the total thiamine content of whole blood is present as TDP, the biologically active form of thiamine; thus, whole blood TDP measures provide a good indication of the adequacy of thiamine body stores (Mancinelli et al, 2003).…”

Section: Assessment Of Thiamine In Whole Bloodmentioning

confidence: 99%

Signs of Preclinical Wernicke's Encephalopathy and Thiamine Levels as Predictors of Neuropsychological Deficits in Alcoholism without Korsakoff's Syndrome

Pitel

Zahr

Jackson

et al. 2010

Neuropsychopharmacol

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The purpose of this study was to determine whether meeting historical criteria for unsuspected Wernicke's encephalopathy (WE), largely under-diagnosed in vivo, explains why some alcoholics have severe neuropsychological deficits, whereas others, with a similar drinking history, exhibit preserved performance. Demographic, clinical, alcohol related, and neuropsychological measures were collected in 56 abstinent alcoholics and 38 non-alcohol-dependent volunteers. Alcoholics were classified using the clinical criteria established by Caine et al (1997) and validated in their neuropathological study of alcoholic cases. Our alcoholics who met a single criterion were considered 'at risk for WE' and those with two or more criteria with 'signs of WE'. Whole blood thiamine was also measured in 22 of the comparison group and 28 alcoholics. Of the alcoholics examined, 27% met no criteria, 57% were at risk for WE, and 16% had signs of WE. Neuropsychological performance of the alcoholic subgroups was graded, with those meeting zero criteria not differing from controls, those meeting one criterion presenting mild-to-moderate deficits on some of the functional domains, and those meeting two or more criteria having the most severe deficits on each of the domains examined. Thiamine levels were selectively related to memory performance in the alcoholics. Preclinical signs of WE can be diagnosed in vivo, enabling the identification of ostensibly 'uncomplicated' alcoholics who are at risk for neuropsychological complications. The graded effects in neuropsychological performance suggest that the presence of signs of WE explains, at least partially, the heterogeneity of alcoholism-related cognitive and motor deficits.

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Section: Assessment Of Thiamine In Whole Bloodmentioning

confidence: 99%

Signs of Preclinical Wernicke's Encephalopathy and Thiamine Levels as Predictors of Neuropsychological Deficits in Alcoholism without Korsakoff's Syndrome

Pitel

Zahr

Jackson

et al. 2010

Neuropsychopharmacol

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“…They were then quantified using a high performance liquid chromatograph (HPLC) system as described by Brown et al (1998b) and Mancinelli et al (2003) with slight modifications. The HPLC system consisted of a delivery system pump (Model 506A, Beckman Instruments Inc., San Ramon, California) equipped with a 20 μl injection loop connected to a 4.6 mm×150 mm NH (aminopropyl-bonded silica gels, 5 μm bead size; Showa Denko, Japan) Shodex column coupled with NH 2 packed guard column.…”

Section: Thiamine Analysismentioning

confidence: 99%

Egg thiamine concentration affects embryo survival in Lake Erie walleye

et al. 2010

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We quantified thiamine in eggs of Lake Erie walleye to determine if differences exist between spawning stocks within the Maumee and Sandusky rivers, both of which drain into the Western Basin. In spring 2004, eggs of walleye were collected in the Maumee River at three occasions (early, peak and late of the spawning run) and in the Sandusky River during the spawning run. After collection, eggs were fertilized with a known amount of milt, incubated and embryo survival was determined at the pigmented eyed stage. Thiamine and its derivatives were analyzed using high performance liquid chromatography. Thiamine pyrophosphate (TPP) was the most abundant form of vitamin B 1 present in eggs (60-95% of the total vitamin B 1 concentration). Total thiamine concentrations in walleye eggs from the Maumee and Sandusky rivers at the peak of the spawning run averaged 6.1±1.6 nmolg −1 and 5.0±2.9 nmolg −1 , respectively. Our results also indicated that Maumee River stock survival to the eyed stage embryo declined as the spawning season progressed (72%, 59% and 37% in the early, middle and late of spawning run, respectively) as well as the total thiamine and TPP concentrations. At the peak of the spawning run, survival to the eyed stage embryo did not differ significantly between stocks 59% versus 65% in the Maumee and Sandusky rivers, respectively, and thiamine concentrations were not significantly different between sites.

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“…Vinas et al [9] concluded that isocratic or gradient elution of thiamine derivatives with ODS column were not satisfactory. Alternatively, separation on a more polar columns (such as NH2−) has been evaluated, with good separation of TMP and TDP thiochromes but extraction of sample is time consuming and the most interesting vitamer (TDP) exhibits a relatively long retention time [10]. Vinas et al [9] have recently shown that a new type of column packing (RP-amide C16) constituted a good alternative to ion pairing or polar columns to analyze underivatized thiamine vitamers; however, this technique was used with UV detection (234 nm/266 nm) and was therefore of limited sensitivity and prone to considerable interferences in biological fluids such as urine.…”

Section: Introductionmentioning

confidence: 99%

Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column

Batifoulier¹,

Verny²,

Besson³

et al. 2005

Journal of Chromatography B

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Simultaneous liquid chromatographic assessment of thiamine, thiamine monophosphate and thiamine diphosphate in human erythrocytes: a study on alcoholics

Cited by 76 publications

References 24 publications

Signs of Preclinical Wernicke's Encephalopathy and Thiamine Levels as Predictors of Neuropsychological Deficits in Alcoholism without Korsakoff's Syndrome

Signs of Preclinical Wernicke's Encephalopathy and Thiamine Levels as Predictors of Neuropsychological Deficits in Alcoholism without Korsakoff's Syndrome

Egg thiamine concentration affects embryo survival in Lake Erie walleye

Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column

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