Simultaneous multiplexed detection of exosomal microRNAs and surface proteins for prostate cancer diagnosis

Cho, Seongcheol; Yang, Hee Cheol; Rhee, Won Jong

doi:10.1016/j.bios.2019.111749

Cited by 116 publications

(80 citation statements)

References 32 publications

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“…A variety of proteins have been observed in exosomes, including cytoskeletal proteins, tetraspanins (CD9, CD63, CD81 and CD82), ESCRT-associated components (Alix and TSG101), heat shock proteins (HSP60, HSP70, and HSP90), antigen presentation proteins (MHC I and MHC II), and integrins [ 7 , 100 ]. Moreover, some disease-related proteins, including Ep-CAM, epidermal growth factor receptor (EGFR), survivin, and IGF-1R, which are distributed on the surface of exosomes, can be used as biomarkers in clinical diagnosis and prognosis [ 101 ]. It is appealing that these proteins can be used both as biomarkers in biofluids and for the isolation and purification techniques introduced above.…”

Section: Characteristics Of Exosomes In Cancermentioning

confidence: 99%

Isolation and characterization of exosomes for cancer research

et al. 2020

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Exosomes are a subset of extracellular vesicles that carry specific combinations of proteins, nucleic acids, metabolites, and lipids. Mounting evidence suggests that exosomes participate in intercellular communication and act as important molecular vehicles in the regulation of numerous physiological and pathological processes, including cancer development. Exosomes are released by various cell types under both normal and pathological conditions, and they can be found in multiple bodily fluids. Moreover, exosomes carrying a wide variety of important macromolecules provide a window into altered cellular or tissue states. Their presence in biological fluids renders them an attractive, minimally invasive approach for liquid biopsies with potential biomarkers for cancer diagnosis, prediction, and surveillance. Due to their biocompatibility and low immunogenicity and cytotoxicity, exosomes have potential clinical applications in the development of innovative therapeutic approaches. Here, we summarize recent advances in various technologies for exosome isolation for cancer research. We outline the functions of exosomes in regulating tumor metastasis, drug resistance, and immune modulation in the context of cancer development. Finally, we discuss prospects and challenges for the clinical development of exosome-based liquid biopsies and therapeutics.

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Section: Characteristics Of Exosomes In Cancermentioning

confidence: 99%

Isolation and characterization of exosomes for cancer research

et al. 2020

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“…Additionally, the group proposed the use of additional exosomal surface biomarkers, such as EpCAM, EGFR, survivin, and IGF-1R as pancreatic cancer-associated markers. The fluorescent values from the detection of the surface markers were normalized by the fluorescent intensity of CD63, the initial selection marker for the exosomes [146]. Although the method provided promising results, there are many aspects regarding normalization, the applicability in human biofluid samples, and the accessibility of the detection methods, which require further development.…”

Section: Applications Of Surface-enhanced Raman Scattering (Sers) Basmentioning

confidence: 99%

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Drula

Ott

Berindan‐Neagoe

et al. 2020

Cancers

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Liquid biopsies have become a convenient tool in cancer diagnostics, real-time disease monitoring, and evaluation of residual disease. Yet, the information still encrypted in the variety of tumor-derived molecules identified in biofluids has proven difficult to decipher due to the technological limitations imposed by their biological nature. Such is the case of extracellular vesicle (EV) encapsulated ncRNAs, which have gained traction in recent years as biomarkers. Due to their resilience towards degrading factors they may act as suitable disease indicators. This review addresses the less described issues in this context. We present an overview of less investigated biofluids that can be used for EV isolation in addition to different isolation approaches to overcome the technical challenges these specimens harbor. Furthermore, we summarize the latest technological advances providing improvement to ncRNA detection and analysis. Thereby, this review summarizes the current state-of-the-art methodologies regarding EV and EV derived miRNA analysis and how they compare to current approaches.

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“…Moreover, these studies demonstrated that miRNAs in body fluids remained stable under various extreme conditions, such as boiling, a very low or high pH, repeated freeze-thaw, and storage at room temperature for a long time. Besides, thanks to the high sensitivity and speed of the new profiling techniques of molecular biology, today it is much easier to study the profile of the global miRNA level, rather than that of proteins or secreted metabolites [37,38].…”

Section: Profiling Evs-mirnasmentioning

confidence: 99%

Extracellular Vesicles: New Endogenous Shuttles for miRNAs in Cancer Diagnosis and Therapy?

Martellucci

Orefice

Angelucci

et al. 2020

IJMS

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Extracellular Vesicles (EVs) represent a heterogeneous population of membranous cell-derived structures, including cargo-oriented exosomes and microvesicles. EVs are functionally associated with intercellular communication and play an essential role in multiple physiopathological conditions. Shedding of EVs is frequently increased in malignancies and their content, including proteins and nucleic acids, altered during carcinogenesis and cancer progression. EVs-mediated intercellular communication between tumor cells and between tumor and stromal cells can modulate, through cargo miRNA, the survival, progression, and drug resistance in cancer conditions. These consolidated suggestions and EVs’ stability in bodily fluids have led to extensive investigations on the potential employment of circulating EVs-derived miRNAs as tumor biomarkers and potential therapeutic vehicles. In this review, we highlight the current knowledge about circulating EVs-miRNAs in human cancer and the application limits of these tools, discussing their clinical utility and challenges in functions such as in biomarkers and instruments for diagnosis, prognosis, and therapy.

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Simultaneous multiplexed detection of exosomal microRNAs and surface proteins for prostate cancer diagnosis

Cited by 116 publications

References 32 publications

Isolation and characterization of exosomes for cancer research

Isolation and characterization of exosomes for cancer research

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Extracellular Vesicles: New Endogenous Shuttles for miRNAs in Cancer Diagnosis and Therapy?

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