Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays

Tomagra, Giulia; Franchino, Claudio; Pasquarelli, A.; Carbone, Emilio; Olivero, P.; Carabelli, Valentina; Picollo, F.

doi:10.1016/j.bpc.2019.106241

Cited by 9 publications

(12 citation statements)

References 36 publications

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“…The main focus was to achieve low detection limits, due to the fact that concentration of DA is extremely low in patients with neurological diseases [ 39 ]. In this context, PC-12 cell lines play an important role in the detection of DA in vivo, with several optical [ 40 , 41 , 42 ], electrochemical biosensing systems, fixed potential amperometry [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] and voltametric ones [ 52 , 53 , 54 ], and electrical devices [ 55 , 56 ] being investigated, with great potential for diagnostic purposes being demonstrated.…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

“…Several electrochemical biosensors were reported for DA detection in PC-12 cells, most of them using fixed potential amperometry [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] and voltammetry [ 52 , 53 , 54 ].…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

“…Other amperometric arrays use a microfluidic sensor based on carbon nanotubes (CNTs)-modified indium tin oxide (ITO) microelectrodes for DA detection in single living rat pheochromocytoma (PC 12) cells [ 48 ], while the microdialysis-amperometric system reported in [ 49 ] uses high-performance liquid chromatography with electrochemical detection (HPLC–EC) [ 49 ], and the scale-integrated amperometric sensor in [ 50 ] was able to image simultaneously the DA release from PC-12 cells. Besides the amperometric detection assays exemplified above, which operated at relatively low overvoltage of around +0.3 V, another assay based on micro graphitic-diamond multi electrode arrays was applied for the detection of DA released form PC-12 cells under K + stimulation at a higher overvoltage of +0.65 V [ 51 ].…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

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PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

et al. 2022

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PC-12 cells have been widely used as a neuronal line study model in many biosensing devices, mainly due to the neurogenic characteristics acquired after differentiation, such as high level of secreted neurotransmitter, neuron morphology characterized by neurite outgrowth, and expression of ion and neurotransmitter receptors. For understanding the pathophysiology processes involved in brain disorders, PC-12 cell line is extensively assessed in neuroscience research, including studies on neurotoxicity, neuroprotection, or neurosecretion. Various analytical technologies have been developed to investigate physicochemical processes and the biosensors based on optical and electrochemical techniques, among others, have been at the forefront of this development. This article summarizes the application of different biosensors in PC-12 cell cultures and presents the modern approaches employed in neuronal networks biosensing.

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Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

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PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

et al. 2022

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“…Also, in this case, the modulation of the penetration depth of the ions is controlled by metal deposition on the diamond surface. This approach allows implanting simultaneously multiple electrodes, fabricating a single sensor per irradiation, but a complex procedure is mandatory to properly align the two masks [77,79,107,108]. Both approaches are schematically described in Fig.…”

Section: Graphitization By Ion Beam Lithographymentioning

confidence: 99%

Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes

Kühn¹,

Picollo

Carabelli

et al. 2020

Pflugers Arch - Eur J Physiol

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To understand the working principles of the nervous system is key to figure out its electrical activity and how this activity spreads along the neuronal network. It is therefore crucial to develop advanced techniques aimed to record in real time the electrical activity, from compartments of single neurons to populations of neurons, to understand how higher functions emerge from coordinated activity. To record from single neurons, a technique will be presented to fabricate patch pipettes able to seal on any membrane with a single glass type and whose shanks can be widened as desired. This dramatically reduces access resistance during whole-cell recording allowing fast intracellular and, if required, extracellular perfusion. To simultaneously record from many neurons, biocompatible probes will be described employing multi-electrodes made with novel technologies, based on diamond substrates. These probes also allow to synchronously record exocytosis and neuronal excitability and to stimulate neurons. Finally, to achieve even higher spatial resolution, it will be shown how voltage imaging, employing fast voltage-sensitive dyes and two-photon microscopy, is able to sample voltage oscillations in the brain spatially resolved and voltage changes in dendrites of single neurons at millisecond and micrometre resolution in awake animals.

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“…These studies have proved that PC12 cells share many electrophysiological features with neurons. Some MEA-based studies on the release of neurotransmitters of PC12 cells have been reported [13][14][15], and a study on the action potentials of PC12 cells has just been published recently [16]. However, no research has been performed to compare the electrical excitability of PC12 quasi-neuronal networks with that of normal neuronal networks yet.…”

Section: Introductionmentioning

confidence: 99%

Study on influence of external factors on the electrical excitability of PC12 quasi-neuronal networks through Voltage Threshold Measurement Method

Lü

Meng

et al. 2022

PLoS ONE

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The aim of this paper was to investigate the influence of four different external factors (acetylcholine, ethanol, temperature and lidocaine hydrochloride) on PC12 quasi-neuronal networks by multielectrode-array-based Voltage Threshold Measurement Method (VTMM). At first, VTMM was employed to measure the lowest amplitude of the voltage stimulating pulses that could just trigger the action potential from PC12 quasi-neuronal networks under normal conditions, and the amplitude was defined as the normal voltage threshold (VTh). Then the changes of the VTh of PC12 quasi-neuronal networks treated by the four external factors were tested respectively. The results showed the normal VTh of PC12 quasi-neuronal networks was 36 mV. The VTh has negative correlation with the concentration of acetylcholine and has positive correlation with the concentration of ethanol. The curves of the correlation of the VTh with temperature and the concentration of lidocaine hydrochloride were U-shaped and Λ-shaped respectively. Comparing with our earlier studies on hippocampal neuronal networks and hippocampal slices, PC12 quasi-neuronal networks not only had the same typical voltage threshold characteristic, but also had similar changes on electrical excitability when treated by the four external factors mentioned above. Therefore, the rapid-formed PC12 quasi-neuronal networks could replace neuronal networks in proper conditions, and VTMM could be used to analyze the influence of external factors on the electrical excitability of PC12 quasi-neuronal networks.

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Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays

Cited by 9 publications

References 36 publications

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes

Study on influence of external factors on the electrical excitability of PC12 quasi-neuronal networks through Voltage Threshold Measurement Method

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