Simultaneous Profiling of mRNA Transcriptome and DNA Methylome from a Single Cell

A Comparative Overview of Epigenomic Profiling Methods

“…Many of them have been adapted to single-cell resolution. Various methods simultaneously measure DNA methylome and the transcriptome (Angermueller et al, 2016;Hou et al, 2016;Hu Y. et al, 2016;Hu et al, 2019), chromatin accessibility and the transcriptome (Cao et al, 2018;Liu et al, 2019;Zhu et al, 2019), or chromatin accessibility, the transcriptome, and cell surface epitopes (Swanson et al, 2021) at single-cell resolution, which are rapidly advancing in scope and performance. Here, we only focus on techniques for simultaneous measurement of multiple epigenomic layers and provide an overview of them.…”

Advances in single-cell multi-omics methods and their applications in developmental biology

“…转录组与表观遗传组联合分析, 能够深入研究基 因表达的表观遗传调控规律. scMT-seq [25] , scM&Tseq [27] 和sc-GEM [26] 等测序方法整合了单细胞转录组和 DNA甲基化组测序技术, 使研究者能够分析细胞中 mRNA的可变剪接与DNA甲基化间的关系; 而sci-CAR-seq [38] , SNARE-seq [17] , scCAT-seq [39] , ATAC-RNA-seq [19] 和Paired-seq [20] 等技术能在单细胞中同时 获得染色质可及性和转录组数据, 使研究者可以在单 细胞分辨率下研究基因组顺式调控元件和反式调节因 子在细胞命运决定中的作用; CITE-seq [28] , REAPseq [29] , PLAYR [40] 和PEA/STA [41] 等技术将单细胞转录…”

The Cancer Epigenome: A Review

“…Methylation generally silences a gene by prohibiting its transcription. The profiling of DNA methylation patterns is called the methylome [54] and has been mapped [55], as well as used in concert with transcriptome profiling to investigate and define the larger epigenetic landscape [56]. Finally, DNA methylation is reversible with the removal of the methyl group.…”