Simultaneous quantification of 19 nonsteroidal anti‐inflammatory drugs in oral fluid by liquid chromatography‐high resolution mass spectrometry: Application on ultratrail runner's oral fluid

Mashal, Mohammad Shafiq; Nalin, Marie; Bévalot, Fabien; Sallet, Pierre; Guitton, Jérôme; Machon, Christelle

doi:10.1002/dta.3216

Cited by 4 publications

(7 citation statements)

References 54 publications

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“…The dry residue was resuspended in 100‐μl mobile phase, and 10 μl were injected into the liquid chromatography–mass spectrometry/high‐resolution mass spectrometer (LC–MS/HRMS) device. OF samples were prepared as previously described 9 …”

Section: Methodsmentioning

confidence: 99%

“…Analyses were carried out on an UltiMate 3000 system coupled with a Q‐Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific™, Bremen, Germany). LC–MS/HRMS conditions were as previously described 9 . Quantification was performed using internal standard method (Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…LC–MS/HRMS conditions were as previously described. 9 Quantification was performed using internal standard method (Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the samples must be based on a simple device, a low or no invasive procedure and must be rapidly executed. In this context, a method for the quantification of 19 NSAIDs in oral fluid (OF) was previously validated 9 . However, the transport and the storage of OF samples may present some difficulties when it is abroad.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, a method for the quantification of 19 NSAIDs in oral fluid (OF) was previously validated. 9 However, the transport and the storage of OF samples may present some difficulties when it is abroad. An alternative is the use of dried blood spot (DBS).…”

Section: Introductionmentioning

confidence: 99%

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Comparative study between direct analysis in whole blood, oral fluid, and declaration of consumption for the prevalence of nonsteroidal anti‐inflammatory drugs and acetaminophen in ultratrail runners

Mashal

Bévalot

Citterio‐Quentin

et al. 2022

Drug Testing and Analysis

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Ultratrail running is a sport with growing number of adherents. To complete ultratrail despite physical issues such as joint and muscle pain, many runners use nonsteroidal anti‐inflammatory drugs (NSAIDs) and acetaminophen. Studies asking participants about their consumption of drugs during ultratrail revealed a prevalence of NSAIDs and acetaminophen up to 70% and 25%, respectively. The aims of the present study were to determine the prevalence of NSAIDs and acetaminophen for 81 runners during the 2021 Ultratrail du Mont Blanc® (UTMB®) using direct analysis of dried blood spots (DBS) and oral fluid (OF) and to compare results with the declaration of consumption by runners; this is to identify the most relevant method to study the prevalence of drugs. Our results show a prevalence of NSAIDs of 46.6% using DBS, 18.5% using OF, and 13.8% based on a questionnaire. Prevalence of acetaminophen were 30.1%, 30.9%, and 22.5% using DBS, OF, and questionnaire, respectively. From this study, we conclude that the analysis of drugs directly in DBS is the most relevant tool to determine the prevalence in ultratrail events.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…LC–MS/HRMS conditions were as previously described. 9 Quantification was performed using internal standard method (Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative study between direct analysis in whole blood, oral fluid, and declaration of consumption for the prevalence of nonsteroidal anti‐inflammatory drugs and acetaminophen in ultratrail runners

Mashal

Bévalot

Citterio‐Quentin

et al. 2022

Drug Testing and Analysis

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Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

Ong

Green

Rowland

et al. 2023

Analytical Science Advances

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Research in sport and exercise science (SES) is reliant on robust analyses of biomarker measurements to assist with the interpretation of physiological outcomes. Mass spectrometry (MS) is an analytical approach capable of highly sensitive, specific, precise, and accurate analyses of a range of biomolecules, many of which are of interest in SES including, but not limited to, endogenous metabolites, exogenously administered compounds (e.g. supplements), mineral ions, and circulating/tissue proteins. This annual review provides a summary of the applications of MS across studies investigating aspects related to sport or exercise in manuscripts published, or currently in press, in 2022. In total, 93 publications are included and categorized according to their methodologies including targeted analyses, metabolomics, lipidomics, proteomics, and isotope ratio/elemental MS. The advantageous analytical opportunities afforded by MS technologies are discussed across a selection of relevant articles. In addition, considerations for the future of MS in SES, including the need to improve the reporting of assay characteristics and validation data, are discussed, alongside the recommendation for selected current methods to be superseded by MS‐based approaches where appropriate. The review identifies that a targeted, mostly quantitative, approach is the most commonly applied MS approach within SES, although there has also been a keen interest in the use of ‘omics’ to perform hypothesis‐generating research studies. Nonetheless, MS is not commonplace in SES at this time, but its use to expand, and possibly improve, the analytical options should be continually considered to exploit the benefits of analytical chemistry in exercise/sports‐based research. Overall, it is exciting to see the gradually increasing adoption of MS in SES and it is expected that the number, and quality, of MS‐based assays in SES will increase over time, with the potential for 2023 to further establish this technique within the field.

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Quantitative analysis of nonsteroidal anti‐inflammatory drugs in dried blood spot from mountain ultra‐trail runners. Contribution of pharmacokinetic models for the interpretation of the results

Mashal,

Guitton,

Sallet

et al. 2024

Drug Testing and Analysis

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Monitoring of drug use in athletes is of interest both for health and competition‐related issues. Considering the advantages of Dried Blood Sampling (low invasiveness, easy sampling, long term storage), we have validated a quantitative LC–MS/HRMS method for the screening of 16 nonsteroidal anti‐inflammatory drugs. For all drugs, accuracy and imprecision were within 15% for the 3 levels of quality control and lower than 20% for the lower limit of quantification. Application was performed from samples obtained for Ultra‐Trail du Mont‐Blanc® 2021 and 2022. A focus on ibuprofen and its metabolites (hydroxyibuprofen, carboxyibuprofen, ibuprofen glucuronide and hydroxyibuprofen glucuronide) was made because the results showed that it was the most detected nonsteroidal anti‐inflammatory drug. Further, an interpretation of the ibuprofen concentrations was proposed either from experimental data obtained after an intake of ibuprofen by 10 control subjects, or from a pharmacokinetic modelling and simulations. Depending on the analytical performances of the method, we proposed possible detection windows for ibuprofen in runners. The pharmacokinetic model made it possible to consider two scenarios with and without modification of the total clearance of ibuprofen linked to a modification of the pharmacokinetics of the drugs due to the practice of a long and intense physical activity.

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Simultaneous quantification of 19 nonsteroidal anti‐inflammatory drugs in oral fluid by liquid chromatography‐high resolution mass spectrometry: Application on ultratrail runner's oral fluid

Cited by 4 publications

References 54 publications

Comparative study between direct analysis in whole blood, oral fluid, and declaration of consumption for the prevalence of nonsteroidal anti‐inflammatory drugs and acetaminophen in ultratrail runners

Comparative study between direct analysis in whole blood, oral fluid, and declaration of consumption for the prevalence of nonsteroidal anti‐inflammatory drugs and acetaminophen in ultratrail runners

Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

Quantitative analysis of nonsteroidal anti‐inflammatory drugs in dried blood spot from mountain ultra‐trail runners. Contribution of pharmacokinetic models for the interpretation of the results

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