Simultaneous Quantification of Cells and Nanomaterials by Inductive-Coupled Plasma Techniques

Albanese, Alexandre; Tsoi, Kim; Chan, Warren C. W.

doi:10.1177/2211068212457039

Cited by 22 publications

(24 citation statements)

References 18 publications

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“…Quantum dot concentrations were converted from measured Cd concentrations using Cd‐quantum dot calibration curves established for each quantum dot size. Cell numbers were converted from measured Mg 2+ concentrations using Mg 2+ calibration curves with known numbers of cells using the technique developed by Albanese et al The effects of intracellular quantum dots on cell viability were determined by the Cell Proliferation Kit II (Roche Applied Science). Their values were expressed as percentage cell viability of non‐treated cells with identical culture conditions.…”

Section: Methodsmentioning

confidence: 99%

The Role of Ligand Density and Size in Mediating Quantum Dot Nuclear Transport

Tang

Sathiamoorthy

Lustig

et al. 2014

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Studying the effects of the physicochemical properties of nanomaterials on cellular uptake, toxicity, and exocytosis can provide the foundation for designing safer and more effective nanoparticles for clinical applications. However, an understanding of the effects of these properties on subcellular transport, accumulation, and distribution remains limited. The present study investigates the effects of surface density and particle size of semiconductor quantum dots on cellular uptake as well as nuclear transport kinetics, retention, and accumulation. The current work illustrates that cellular uptake and nuclear accumulation of nanoparticles depend on surface density of the nuclear localization signal (NLS) peptides with nuclear transport reaching a plateau at 20% surface NLS density in as little as 30 min. These intracellular nanoparticles have no effects on cell viability up to 72 h post treatment. These findings will set a foundation for engineering more sophisticated nanoparticle systems for imaging and manipulating genetic targets in the nucleus.

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Section: Methodsmentioning

confidence: 99%

The Role of Ligand Density and Size in Mediating Quantum Dot Nuclear Transport

Tang

Sathiamoorthy

Lustig

et al. 2014

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“…Statistical comparisons are shown as follows: **p < 0.01, *p < 0.05, N.S: not significant. References (29)(30)(31)…”

Section: Figmentioning

confidence: 99%

DNA-controlled dynamic colloidal nanoparticle systems for mediating cellular interaction

Ohta

Glancy

Chan

2016

Science

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186

164

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Precise control of biosystems requires development of materials that can dynamically change physicochemical properties. Inspired by the ability of proteins to alter their conformation to mediate function, we explored the use of DNA as molecular keys to assemble and transform colloidal nanoparticle systems. The systems consist of a core nanoparticle surrounded by small satellites, the conformation of which can be transformed in response to DNA via a toe-hold displacement mechanism. The conformational changes can alter the optical properties and biological interactions of the assembled nanosystem. Photoluminescent signal is altered by changes in fluorophore-modified particle distance, whereas cellular targeting efficiency is increased 2.5 times by changing the surface display of targeting ligands. These concepts provide strategies for engineering dynamic nanotechnology systems for navigating complex biological environments.

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“…The method is capable of quantifying specific chemical elements of NMs in cultured cells and tissues with ppb accuracy. 14,15 Mass spectrometry (MS) is another rather sensitive technique for cellular NM quantitation. The elements of analyzed particles are ionized and separated by their mass-to-charge-ratio.…”

Section: Label-free Dosimetry and Imaging Techniques-toward High Thromentioning

confidence: 99%

“…AES is based on the emission of radiation, which is characteristic for every element. The method is capable of quantifying specific chemical elements of NMs in cultured cells and tissues with ppb accuracy …”

Section: Label‐free Dosimetry and Imaging Techniques—toward High Throughputmentioning

confidence: 99%

High throughput toxicity screening and intracellular detection of nanomaterials

Collins

Annangi

Rubio

et al. 2016

WIREs Nanomed Nanobiotechnol

117

106

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With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read‐across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter‐experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM‐cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose‐ and time‐dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label‐free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance‐based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1413. doi: 10.1002/wnan.1413For further resources related to this article, please visit the WIREs website.

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Simultaneous Quantification of Cells and Nanomaterials by Inductive-Coupled Plasma Techniques

Cited by 22 publications

References 18 publications

The Role of Ligand Density and Size in Mediating Quantum Dot Nuclear Transport

The Role of Ligand Density and Size in Mediating Quantum Dot Nuclear Transport

DNA-controlled dynamic colloidal nanoparticle systems for mediating cellular interaction

High throughput toxicity screening and intracellular detection of nanomaterials

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