Simultaneous quantification of individual intermediate steroids in silkworm ecdysone biosynthesis by liquid chromatography–tandem mass spectrometry with multiple reaction monitoring

Hikiba, Juri; Ogihara, Mari H.; Iga, Masatoshi; Saito, Kazuki; Fujimoto, Yoshinori; Suzuki, Minoru; Kataoka, Hiroshi

doi:10.1016/j.jchromb.2012.12.014

Cited by 44 publications

(35 citation statements)

References 23 publications

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“…Samples were centrifuged and supernatant was collected. The pellet was twice re-extracted with 1 ml methanol for 1 hour, the extracts combined, cleaned by solid-phase extraction, dried down and analyzed by LC-MS/MS as described in (Hikiba et al 2013) with minor modifications. The detection limit of the method was ca 5 pg per individual ecdysteroid.…”

Section: Methodsmentioning

confidence: 99%

Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development

Rodenfels

Lavrynenko

Ayciriex

et al. 2014

Preprint

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In Drosophila larvae, growth and developmental timing are regulated by nutrition in a tightly coordinated fashion. The networks that couple these processes are far from understood. Here, we show that the intestine responds to nutrient availability by regulating production of a circulating lipoprotein-associated form of the signaling protein Hedgehog (Hh). Levels of circulating Hh tune the rates of growth and developmental timing in a coordinated fashion. Circulating Hh signals to the fat body to control larval growth. It regulates developmental timing by controlling ecdysteroid production in the prothoracic gland. Circulating Hh is especially important during starvation, when it is also required for mobilization of fat body triacylglycerol (TAG) stores. Thus, we demonstrate that Hh, previously known only for its local morphogenetic functions, also acts as a lipoprotein-associated endocrine hormone, coordinating the response of multiple tissues to nutrient availability.

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Section: Methodsmentioning

confidence: 99%

Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development

Rodenfels

Lavrynenko

Ayciriex

et al. 2014

Preprint

View full text Add to dashboard Cite

show abstract

“…Methods for LC-MS/MS detection of ecdysteroids have been developed (Blais et al, 2010;Hikiba et al, 2013;Li et al, 2006;Miyashita et al, 2011). However, crude larval extracts are difficult to analyze because of the abundance of other lipids.…”

Section: The Composition Of the Drosophila Ecdysteroidomementioning

confidence: 99%

The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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Ecdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol.

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“…The liquid chromatography mass spectrometry ecdysone analyses were performed with the liquid chromatography instrument UHPLC Agilent Technologies 1290 Infinity (Santa Clara, California, USA) in combination with an Agilent triple quadruple 6430 mass spectrometer with electrospray ionization (Santa Clara, California, USA) (adapted from [31,32]). The system operation was controlled and processed with the software MassHunter B06.00.…”

Section: Methodsmentioning

confidence: 99%

Proximate mechanism of behavioral manipulation of an orb-weaver spider host by a parasitoid wasp

et al. 2017

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Some ichneumonid wasps induce modifications in the web building behavior of their spider hosts to produce resistant “cocoon” webs. These structures hold and protect the wasp’s cocoon during pupa development. The mechanism responsible for host manipulation probably involves the inoculation of psychotropic chemicals by the parasitoid larva during a specific developmental period. Recent studies indicate that some spiders build cocoon webs similar to those normally built immediately before ecdysis, suggesting that this substance might be a molting hormone or a precursor chemical of this hormone. Here, we report that Cyclosa spider species exhibiting modified behavior presented higher 20-OH-ecdysone levels than parasitized spiders acting normally or unparasitized individuals. We suggest that the lack of control that spiders have when constructing modified webs can be triggered by anachronic activation of ecdysis.

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Simultaneous quantification of individual intermediate steroids in silkworm ecdysone biosynthesis by liquid chromatography–tandem mass spectrometry with multiple reaction monitoring

Cited by 44 publications

References 23 publications

Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development

Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development

The Ecdysteroidome of Drosophila: influence of diet and development

Proximate mechanism of behavioral manipulation of an orb-weaver spider host by a parasitoid wasp

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