Simultaneous quantitative analysis of nine constituents in six Chinese medicinal materials from Citrus genus by high‐performance liquid chromatography and high‐resolution mass spectrometry combined with chemometric methods

Abstract

In this study, we aim to determine the chemical constituents of six Chinese medicinal materials from the Citrus genus using high‐performance liquid chromatography and high‐resolution mass spectrometry. Eight flavonoids and one coumarin were identified and further quantified as marker substances by high‐performance liquid chromatography method. The separation was performed on an Agilent TC‐C18 column with 0.1% formic acid and acetonitrile as the mobile phase under gradient elution. The analytical method was fully validated in terms of linearity, sensitivity, intra‐ and inter‐day precision and repeatability, limit of detection, limit of quantitation, and recovery. It was subsequently applied to evaluate the quality of 103 batches of the Chinese medicinal materials from the Citrus genus. In addition, the principal constituent analysis was used to compare the samples of different species from the Citrus genus leading to successful classification of the samples in accordance with their origins. It was found that the contents of nine constituents varied greatly in different ripening stages and varieties of the samples from the Citrus genus. In addition, neoeriocitrin and 5,7‐dimethoxycoumarin were determined as two unique constituents of ‘Zhiqiao’ and ‘Foshou’, respectively. In conclusion, this study provides a chemical basis for quality control of Chinese medicinal materials from the Citrus genus.

Simultaneous quantitative analysis of ten bioactive flavonoids in Citri Reticulatae Pericarpium Viride (Qing Pi) by ultrahigh‐performance liquid chromatography and high‐resolution mass spectrometry combined with chemometric methods

“…Most of the existing studies on Qing Pi report the comparative analysis of Qing Pi and one or more of medicinal materials of the Citrus genus, mainly using fingerprint and gas chromatography-mass spectrometry methods. [14][15][16][17][18] There is a lack of systematic analysis of Qing Pi with different origins and specifications. Therefore, it is of great significance to establish an effective analytical method to identify and quantify the chemical constituents of Qing Pi, thus determining its origin and specifications.…”

Voltammetric Determination of Hesperidin on the Electrode Modified with SnO2 Nanoparticles and Surfactants

“…In this case, chromatography with different types of detectors is the most often applied method for its identification and determination with high selectivity and acceptable sensitivity. High-performance liquid chromatography (HPLC) with UV- [5][6][7][8], diode-array [9,10] and tandem massspectrometric [11][12][13] detection is usually applied for these purposes. Recently, several approaches based on ultra-HPLC with tandem mass-spectrometric detector [14,15] and high-performance thin-layer chromatography with UV- [16] and densitometric [17] detection have been developed.…”

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO2 fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

“…For example, as a representative simple coumarin, compound 28 generated a parent ion peak at m/z 147.0441 [M+H] + , and its fragment ions included m/z 119.0857 [M+H‐CO] + , 103.0546 [M+H‐CO 2 ] + , and 91.0547 [M+H‐2CO] + [23]. According to the structural fragmentation patterns of coumarins [24], compound 28 was identified as coumarin.…”