Simultaneous quantitative analysis of polyphenolic compounds in human plasma by liquid chromatography tandem mass spectrometry

Abstract

A diet rich in polyphenolic compounds has recognized health benefits, and as such is routinely monitored as part of dietary intervention studies. A method for the simultaneous determination of 36 phenolic compounds, including phenolic acids and flavonoids, using liquid chromatography and tandem mass spectrometry is described here. The target analytes were quantified based on their specific mass spectral fragments using a selected reaction monitoring approach. A C18 column with embedded aromatic functionality ensured separation of all phenolic compounds studied which included several pairs of isomers. Sample preparation involved the use of β‐glucuronidase to release the phenolic compounds from their conjugated forms. The intra‐day and inter‐day precision and accuracy was less than 7% for all phenolic compounds studied. Recoveries, where plasma was spiked with three different concentrations of the analytes, ranged from 95–115%. The limits of detection and quantification were 0.23–3.89 and 1.15–7.79 nM, respectively. The method was successfully applied to real samples and the range reported for each phenolic compound, with the exception of hydroferulic acid, nordihydroguaiaretic acid, methylgallate, and m‐coumaric acid, was at least an order of magnitude higher than the limit of quantification for the method.

Chromatographic identification of “green capping agents” extracted from Nasturtium officinale (Brassicaceae) leaves for the synthesis of MoO3 nanoparticles

“…Different methods have been reported in the literature for determination of phytochemicals via HPLC, MS, UV, etc. [38][39][40][41][42][43]. Moreover the GC-MS provides scientific world a more economic and efficient option to scrutinize key fermentation products such as volatile fatty acids, acetic acid, esters, etc.…”

Measurement and Analysis of Solvent Behavior and Thermodynamic Properties of 3,4,5-Trimethoxycinnamic Acid in Industrial Common Solvents with Different Temperatures (T = 278.15–323.15 K)

“…However, the solubility of TMCA was much lower in some solvents, which limited its industrial application. In previous reports, to obtain higher purity TMCA, high-performance liquid chromatography (HPLC), HPLC/mass spectrometry (HPLC/MS), and other methods were utilized to separate . It is of great scientific value to explore the solubility of TMCA in different solvents and select the appropriate solvent for dissolution or crystallization, providing convenience for future research and application of TMCA.…”

“…In previous reports, to obtain higher purity TMCA, high-performance liquid chromatography (HPLC), HPLC/mass spectrometry (HPLC/MS), and other methods were utilized to separate. 13 It is of great scientific value to explore the solubility of TMCA in different solvents and select the appropriate solvent for dissolution or crystallization, providing convenience for future research and application of TMCA. At present, the relative literature studies available for reference on the solubility data of TMCA are not reported to provide a great reference for TMCA industrial production; the common organic solvents (methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, methyl acetate, ethyl acetate, acetonitrile, and acetone) and water in the pharmaceutical and chemical industries were selected for the crystallization experiment with the temperature range (278.15−323.15 K) in this work.…”