Simultaneous separation and determination of active components in Cordyceps sinensis and Cordyceps militarris by LC/ESI-MS

Huang, Lan-Fang; Liang, Yi‐Zeng; Guo, Fang-Qiu; Zhou, Zhifeng; Cheng, Ben-Mei

doi:10.1016/s0731-7085(03)00415-1

Cited by 93 publications

(48 citation statements)

References 6 publications

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“…+86 236 877 4624, E-Mail winn0324 @ sohu.com erable interest in traditional Chinese medicine as a source of novel therapeutics. It has been demonstrated that adenine, hypoxanthine, adenosine and cordycepin are the major bioactive components in Cordyceps sinensis [2]. Their molecular structures are shown in figure 1.…”

Section: Urologiamentioning

confidence: 99%

Effect of <i>Cordyceps sinensis</i> on Renal Function of Patients with Chronic Allograft Nephropathy

et al. 2011

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Objective: To investigate the effect of Cordyceps sinensis (Bailing Capsule, fermented agent of C. sinensis) on renal function of patients with chronic allograft nephropathy (CAN). Methods: A total of 231 CAN patients who underwent transplantation between 2005 and 2008 and experienced chronic graft dysfunction were randomly divided into 2 groups. Patients in group A (n = 122) were treated with immunosuppressive agents and C. sinensis (2.0 g/day, 3 times a day), while patients in group B (n = 109) were treated with traditional immunosuppressive drugs. Serum creatinine (SCr), blood urea nitrogen (BUN), creatinine clearance rate (C_Cr) and urinary protein in 24 h (24-hour Upro) of all patients were measured before and after treatment. Urinary concentrations of transforming growth factor (TGF)-β₁, retinol-binding protein (RBP) and β₂-microglobulin (β₂-MG) were detected at the same time. Results: After 6-month treatment with C. sinensis, SCr and C_Cr in group A were significantly improved (p < 0.05), while there was no significant improvement observed for group B. There was no significant change in BUN in groups A and B (p > 0.05). 24-hour Upro, RBP and β₂-MG were lower in group A after treatment with C. sinensis (p < 0.05 or p < 0.01), and urinary TGF-β₁ in group A was significantly lower than the values before C. sinensis treatment (p < 0.05), but showed no change in patients of group B. In group A, renal function had improved in 72 cases, stabilized in 38 cases, and worsened in 12 cases. In group B, renal function had improved in 14 cases, stabilized in 50 cases, and worsened in 45 cases (p < 0.05). Conclusion:C. sinensis therapy is advantageous in improving renal function of CAN patients by retarding CAN progression.

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Section: Urologiamentioning

confidence: 99%

Effect of <i>Cordyceps sinensis</i> on Renal Function of Patients with Chronic Allograft Nephropathy

et al. 2011

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“…10 It has been reported that the cordycepin content of C. militaris cultured in China was 0.75%, although the content varied according to cultivated source. 8 Submerged cultivated C. militaris is found to secrete cordycepin into the environment. 20 Cordycepin is found to possess antitumor activity, 21 inhibition of nuclear RNA synthesis in nerve and glial cells, 22 and antiviral activity towards HIV-1 in vitro, 23 as well as antiprotozoal effect 24 and insecticidal activity against Plutella xylostella (L.).…”

Section: Discussionmentioning

confidence: 99%

Comparison of growth‐inhibiting activities of Cordyceps militaris and Paecilomyces japonica cultured on Bombyx mori pupae towards human gastrointestinal bacteria

2006

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The growth-inhibiting activities of materials derived from the fruiting body of Cordyceps militaris cultured on Bombyx mori pupae (CM-1) and pupae separated from the culture (CM-2), and the fruiting body of Paecilomyces japonica cultured on B. mori pupae (PJ-1) and pupae separated from the culture (PJ-2), fresh B. mori larvae (BML), fresh B. mori female pupae (BMP), and Morus alba leaves (MAL) towards eight lactic acid-producing bacteria and 11 harmful intestinal bacteria were examined using an impregnated paper disc bioassay. At 10 mg per disc, methanolic extracts from CM-1 and CM-2 strongly inhibited growth of Clostridium difficile ATCC 9689, C. paraputrificum ATCC 25780, and C. perfringens ATCC 13124 without adverse effects on the growth of eight lactic acid-producing bacteria and the other eight harmful bacteria. The methanolic extracts from PJ-1, PJ-2, BML, BMP, and MAL did not affect growth of all test bacteria. The growth-inhibiting principle of CM-1 and CM-2 towards test clostridia was characterized as cordycepin by spectroscopic analysis. The contents of cordycepin in dried CM-1 and CM-2 were 0.69% and 0.54%, respectively. These results suggest that cordycepin may be produced from the fruiting body of C. militaris cultured on B. mori pupae and then translocated to its host insect and accumulated. Structure-growth inhibition relationships of cordycepin and its eight derivatives against C. perfringens ATCC 13124 indicate that a deoxy form at either 3 or 2 position appears critical for the inhibitory activity. Natural cordycepin and its two analogues, 2 -deoxyadenosine and tubercidin, merit further study as a potential antibacterial agent against various diseases caused by harmful intestinal bacteria such as clostridia.

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“…[1,22] It is now believed that cordycepic acid is, in fact, D-mannitol, and that cordycepin is 3 0 -deoxyadenosine (3…”

Section: Related Species and Artificial Cultivationmentioning

confidence: 99%

“…The linoleic acid content is the highest at 38.44%, followed by the oleic acid, which is 17.94%. The saturated fatty acid content is 42.16%, including C l4 , C l5 , C l6 , C l7 , C l8 , C 20 and C 22 . The palmitic acid and the octadeca acid contents are the highest, and are 21.86% and 15.78%, respectively.…”

Section: Fatty Acids and Metal Elementsmentioning

confidence: 99%

Cordyceps fungi: natural products, pharmacological functions and developmental products

et al. 2009

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Objectives Parasitic Cordyceps fungi, such as Cordyceps sinensis, is a parasitic complex of fungus and caterpillar, which has been used for medicinal purposes for centuries particularly in China, Japan and other Asian countries. This article gives a general idea of the latest developments in C. sinensis research, with regard to the active chemical components, the pharmacological effects and the research and development of products in recent years. Key findings The common names for preparations include DongChongXiaCao in Chinese, winter worm summer grass in English. It has many bioactive components, such as 3 0 -deoxyadenosine, cordycepic acid and Cordyceps polysaccharides. It is commonly used to replenish the kidney and soothe the lung, and for the treatment of fatigue. It also can be used to treat conditions such as night sweating, hyposexuality, hyperglycaemia, hyperlipidaemia, asthenia after severe illness, respiratory disease, renal dysfunction, renal failure, arrhythmias and other heart disease and liver disease. Because of its rarity and outstanding curative effects, several mycelia strains have been isolated from natural Cordyceps and manufactured by fermentation technology, and are commonly sold as health food products. In addition, some substitutes such as C. militaris and adulterants also have been used; therefore, quality control of C. sinensis and its products is very important to ensure their safety and efficacy. Summary Recent research advances in the study of Cordyceps, including Cordyceps mushrooms, chemical components, pharmacological functions and developmental products, has been reviewed and discussed. Developing trends in the field have also been appraised.

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Simultaneous separation and determination of active components in Cordyceps sinensis and Cordyceps militarris by LC/ESI-MS

Cited by 93 publications

References 6 publications

Effect of <i>Cordyceps sinensis</i> on Renal Function of Patients with Chronic Allograft Nephropathy

Effect of <i>Cordyceps sinensis</i> on Renal Function of Patients with Chronic Allograft Nephropathy

Comparison of growth‐inhibiting activities of Cordyceps militaris and Paecilomyces japonica cultured on Bombyx mori pupae towards human gastrointestinal bacteria

Cordyceps fungi: natural products, pharmacological functions and developmental products

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