Simultaneous Separation and Determination of Seven Quinolones Using HPLC: Analysis of Levofloxacin and Moxifloxacin in Plasma and Amniotic Fluid

Nemutlu, Emirhan; Kır, Sedef; Özyüncü, Özgür; Beksaç, Meral

doi:10.1365/s10337-007-0292-9

Cited by 49 publications

(45 citation statements)

References 25 publications

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“…The neutral and zwitterionic forms are expected to get retained by the reverse phase columns. However in aqueous Literature reports present the use of surface response techniques for optimizing the concentration of organic part in the mobile phase 70,71 . The pH of the mobile phase is also an important parameter to be considered and the average pH of analysis was observed 72, 73 between 2.5 to 3.5.…”

Section: Chromatographic Separationmentioning

confidence: 99%

Untitled

2016

IJPSR

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ABSTRACT:Fluoroquinolones are the most commonly prescribed synthetic antibacterial agents having improved pharmacokinetic properties and broad spectrum of activity. Due to their broad clinical activity they are the most promising drug for antibacterial chemotherapy. The current study focused on the application of similar chromatographic system for the analysis of a number of fluoroquinolones from bulk, pharmaceutical formulations and biological matrices. We have also summarized how a single chromatographic system can be used for the quantification of a number of fluoroquinoloes from their respective marketed formulation. A detailed systemic survey of the sample preparation techniques, the chromatographic conditions and detection of the respective fluoroquinolones has been presented in this study. In the course of this discussion the advantages and applicability of the high performance liquid chromatographic techniques are also examined.

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Section: Chromatographic Separationmentioning

confidence: 99%

Untitled

2016

IJPSR

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“…A validated bioanalytical method for quantitative estimation of LEV in human plasma at a pharmacokinetic range approximately 1-20 µg/mL is necessary to conduct the bioequivalence study [5]. A number of analytical methods for quantifying LEV were developed such as highperformance liquid chromatography coupled with ultraviolet (HPLC-UV), fluorescent (HPLC-FL), and tandem mass spectrometry (HPLC-MS/MS) detector [6][7][8][9][10][11][12][13][14][15][16][17]. Most of these method used many kinds of additives as mobile phase component for improving peak shape and resolution i.e.…”

Section: Introductionmentioning

confidence: 99%

“…Most of these method used many kinds of additives as mobile phase component for improving peak shape and resolution i.e. triethylamine [7,[12][13][14], bromide [11], tetramethyl ammonium bromide [8], butadiene styrene brominated ammonium [9], tetrabutylammonium hydrogen sulfate [10], and trifluoroacetic acid [6,15]. The other method was developed under gradient elution using a formic acid 0.05% and methanol as mobile phase component [17], but this method gives relatively long separation time (about 13 min).…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma

et al. 2017

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“…Thorough study of the literature reveals that several methods have been developed for the determination of fluoroquinolones in bulk, pharmaceutical dosage form and biological samples based on wide array of instru-ments like spectrophotometric [9][10][11], HPLC [12,13], capillary electrophoresis [14], electrochemical [15] and spectrofluorimetric [8,[16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Sensitive Spectrofluorimetric Method for Determination of Fluoroquinolones through Charge-Transfer Complex Formation

Shah¹,

Jan²,

Ullah³

et al. 2013

AJAC

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A sensitive spectrofluorimetric method was developed for determination of ciprofloxacin (CPFX), levofloxacin (LEV), gatifloxacin (GAT) and moxifloxacin (MOX) in pure, commercial formulations, human urine and plasma. The method is based on charge-transfer (CT) complex with chloranilic acid. Fluorescence intensity of the complexes was measured at emission wavelength ranging from 445 -492 nm with excitation wavelengths from 285 -330 nm. At optimum experimental conditions, a linear calibration plot was obtained in the concentration range of 20 -1000 ng·mL , 20 -800 ng·mL −1 and 20 -100 ng·mL −1 for CPFX, LEV, MOX and GAT, respectively with good correlation coefficient in the range of 0.9929 -1.0 in methanolic medium. The limit of detection and limit of quantification were found to be 5 ng·mL −1 and 18 ng·mL −1 for CPFX, 12 ng·mL −1 and 40 ng·mL −1 for LEV, 8 ng·mL −1 and 19 ng·mL −1 for MOX, 6 ng·mL −1 and 19 ng·mL −1 for GAT, respectively. The method was found free of interferences from excipients used as additive in pharmaceutical preparations, some common cations and compounds present in urine and plasma as well as co-administered analgesic, vitamins and other drugs. The method was successfully applied for quantification of selected fluoroquinolones in commercial formulations and also in spiked human urine and plasma samples with percent recoveries of 100.0 ± 1.56 and 100.2 ± 1.29 respectively.

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Simultaneous Separation and Determination of Seven Quinolones Using HPLC: Analysis of Levofloxacin and Moxifloxacin in Plasma and Amniotic Fluid

Cited by 49 publications

References 25 publications

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A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma

Sensitive Spectrofluorimetric Method for Determination of Fluoroquinolones through Charge-Transfer Complex Formation

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