Simultaneous Separation and Determination of Quinolones in Pharmaceuticals by Micellar Liquid Chromatography

Abstract

A rapid and simple liquid chromatographic procedure using micellar mobile phases is reported for the separation and determination of four quinolones (pipemidic acid, levofloxacin, norfloxacin and moxifloxacin) in pharmaceuticals.This purpose was achieved without any previous pretreatment step in a C18 column using a micellar mobile phase of 0.15 M sodium dodecyl sulphate, 2.5% propanol and 0.5% triethylamine at pH 3, with retention times below 12 min. For detection, the diode-array UV-Vis set at 276 nm was used. The limits of detection and quantification were between 8-51 and 28-171 ng/mL, respectively. This method was validated in terms of intra-day and inter-day precision and accuracy, and robustness. Calibration curves over the concentration range of 0.1-50 g/mL were linear (r 2 > 0.9997) and. Good claim percentages (96-106 %) were obtained in the analysis of pharmaceutical formulations. The results show that the procedure is suitable for the routine analysis of drugs.

Micellar Liquid Chromatographic Method for Determination of Moxifloxacin and its Impurities

“…A few papers have been published on the topic of determination of fluoroquinolone using micellar HPLC. [24][25] In a published study 24 a method for simultanous separation of four quinolones including moxifloxacin was developed. Also, a study was conducted on the simultaneous separation of levofloxacin and ambroxol.…”

Micellar liquid chromatographic method for the simultaneous determination of Levofloxacin and Ambroxol in combined tablets: Application to biological fluids

“…The literature revealed many methods for the determination of LEV; including a review on the spectrophotometric methods for its determination up to 2008 [6], other specrophotometric methods [7-9], spectrofluorometric methods [10,11], HPLC with UV detection [12,13], fluorometric detection [14], tandem mass spectrometric detection [15]. Capillary electrophoresis with electrochemiluminescence detection [16], electrochemical methods [17], chemiluminescence [18] and flow injection analysis with UV, potentiometric and conductometric detection [19] were also reported.…”

Development of Biomimetic Sensor for Fast and Sensitive Detection of Norfloxacin

“…Other sophisticated analytical techniques reported to identify and quantify fluoroquinones have included surface desorption atmospheric pressure chemical ionization tandem mass spectrometry, micellar liquid chromatography [26] and capillary electrophoresis [27]. Solid phase extraction has been used for sample cleanup and pre-concentration, considering the low concentrations expected in environmental, food and biological samples [21,23,24].…”