Simultaneous separation of cations and anions in capillary electrophoresis

Sáiz, Jorge; Koenka, Israel Joel; Duc, Thanh; Hauser, Peter C.; García-Ruiz, Carmen

doi:10.1016/j.trac.2014.07.015

Cited by 39 publications

(28 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the capacitively coupled contactless conductive detector (C 4 D) was proposed by Zemann et al [5] and da Silva and do Lago [6] in 1998, capillary electrophoresis (CE) and microchip electrophoresis (ME) methods have been applied extensively in the determination of ionic species [7][8][9][10][11][12]. For the CE-C 4 D, many different techniques for concurrent separations have been discussed in a recent review [13]. Wuersig et al reported a simultaneous determination method in short fused silica capillary, the analysis of cations and anions could be carried out by switching the polarity of the high voltage supply.…”

Section: Introductionmentioning

confidence: 99%

Concurrent determination and separation of inorganic cations and anions in microchip electrophoresis with precisely controlled high‐voltage

et al. 2018

View full text Add to dashboard Cite

An improved method for the concurrent determination and separation of cations and anions by microchip electrophoresis with capacitively coupled contactless conductivity detection (ME-C D) is described. Two kinds of microchip structures were designed. The first microchip has a long bent separation channel. And for the defects of the first microchip, the second microchip with a Y-type separation channel has been proposed. The background electrolyte (BGE) composed of 20 mm His/MES and 0.01 mm CTAB was optimized for inhibiting the electroosmotic flow (EOF). Due to the low electroosmotic flow, the cations and anions migrate in opposite directions and can be separated from each other. With the precisely controlled high-voltage, cations and anions can be migrated in microchannels according to our requirements and sequentially detected by a C D detector built in-house. Samples containing K , Na , Li , Cl , F and PO were analyzed simultaneously in a single run (within 140 s) by both methods. The reproducibility obtained by both methods remained below 5% for migration time and within 3.5-9.1% for peak areas. The proposed concurrent determination methods are inexpensive, simple, fast, ease of operation, high degree of integration.

show abstract

Section: Introductionmentioning

confidence: 99%

Concurrent determination and separation of inorganic cations and anions in microchip electrophoresis with precisely controlled high‐voltage

et al. 2018

View full text Add to dashboard Cite

show abstract

“…This is to give time for the operator to calculate the time interval ∆t = bτ/L d that needs to be inserted into the programming of the high voltage distributor before the group of bands reaches the midpoint at x = b/ 2, which is the instant of the first rotation. Simultaneous analysis of cations and anions is very desirable in many applications . The simultaneous electrokinetic injection of a sample in reservoirs 1 and 3 allows the concurrent analysis of cations and anions in the scheme of Fig.…”

Section: Introductionmentioning

confidence: 99%

Number of theoretical plates achievable by a toroidal capillary electrophoresis system

Kist

2017

J of Separation Science

View full text Add to dashboard Cite

The ability of a method and instrument to separate very similar compounds is related to the "plate number," a number indicating performance. The resolution between two neighboring peaks is proportional to the square root of the plate number. Currently available commercial capillary electrophoresis instruments easily reach plate numbers of a few million. In the present work, a capillary electrophoresis system with a toroidal platform is proposed and theoretically studied with the goal of extending the achievable plate number. In this new system, electrophoresis occurs in a nonstop continuous circulating mode within a closed loop capillary (toroid). Plate numbers upwards of one billion are theoretically predicted. This could resolve hundreds of unseparated mixtures of stereoisomers and other analytes that remain without a method for their analysis.

show abstract

“…Moreover, CE showed high suitability for the simultaneous determination of a drug compound and its counter‐ion in a single run. This can easily be achieved in case of cations and slow migrating anions by simply increasing the EOF to force both anions and cations to migrate in the same direction to reach the detector .…”

Section: Separation and Analysis Of Small Moleculesmentioning

confidence: 99%

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

et al. 2016

View full text Add to dashboard Cite

This review updates and follows-up a previous review by highlighting recent advancements regarding capillary electromigration methodologies and applications in pharmaceutical analysis. General approaches such as quality by design as well as sample injection methods and detection sensitivity are discussed. The separation and analysis of drug-related substances, chiral CE, and chiral CE-MS in addition to the determination of physicochemical constants are addressed. The advantages of applying affinity capillary electrophoresis in studying receptor-ligand interactions are highlighted. Finally, current aspects related to the analysis of biopharmaceuticals are reviewed. The present review covers the literature between January 2013 and December 2015.

show abstract

Simultaneous separation of cations and anions in capillary electrophoresis

Cited by 39 publications

References 55 publications

Concurrent determination and separation of inorganic cations and anions in microchip electrophoresis with precisely controlled high‐voltage

Concurrent determination and separation of inorganic cations and anions in microchip electrophoresis with precisely controlled high‐voltage

Number of theoretical plates achievable by a toroidal capillary electrophoresis system

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

Contact Info

Product

Resources

About