“…Similarly, cell decay and EPS destructuration were observed in a membrane bioreactor (MBR) coupled with an anoxic side-stream reactor operating with 10 hours HRT (Fida et al, 2021). Corsino et al (2020b) found that when increasing the HRT in the ASSR from 6 h to 10 h, sludge minimization was mainly driven by endogenous decay, whereas the contribution of the uncoupling metabolism decreased. Nonetheless, Velho and co-authors observed that a 45% increase of the ASSR volume (HRT from 6 h to 10 h) did not promote a further reduction of excess sludge, since it was affected by other factors such as aerobiosis/anaerobiosis alternation (Velho et al, 2016).…”