We have obtained 2552 useful low-resolution spectra of the bright sdBV Balloon 090100001, which form the first large time-resolved spectroscopic dataset of this brightest known pulsating subdwarf B star. The data were obtained at the Nordic Optical Telescope during 7 nights in August/September 2004 over a total time base of 38 nights, aiming to derive pulsational characteristics of this star. In this paper we present the observations and the results obtained from frequency analyses. In our data we find clear evidence for 8 independent frequencies, that were all previously reported in photometric monitoring studies, allowing future asteroseismological studies of this star to be constrained by combined photometric and spectroscopic observations of as much as 8 pulsation modes. We do not find conclusive evidence for new frequencies. We present the first determination of the pulsational radial-velocity amplitudes of this star, and find that the radial-velocity amplitude of the main pulsation mode ( f 1 = 2.80749 mHz) in Balloon 090100001 is 18.9 km s −1 , which is the largest radial-velocity amplitude found in sdB-star pulsations so far. For all spectra, the radial velocities are published electronically.