Simultaneous tracking of fly movement and gene expression using GFP

Grover, Dhruv; Yang, Junsheng; Tavaré, Simon; Tower, John

doi:10.1186/1472-6750-8-93

Cited by 23 publications

(33 citation statements)

References 33 publications

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“…Perhaps controlling the initiation of GFP expression, as with heat-shock promoters, would reduce the possible effects on development, allowing analysis in longitudinal assays (Grover et al, 2008). Caution should be taken when GFP is used in long-term experiments with any drivers.…”

Section: Discussionmentioning

confidence: 99%

Expression of GFP can influence aging and climbing ability in Drosophila

Mawhinney¹,

Staveley²

2011

Genet. Mol. Res.

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ABSTRACT. Green fluorescent protein (GFP) is widely used as a reporter transgene in a variety of organisms. Some of the advantages of using GFP include non-invasive visualization of biological events and/or tissues in live specimens and its benign nature. When GFP is expressed throughout the organism, in neurons and eyes, lifespan and climbing ability of flies are significantly decreased compared to similar crosses with a lacZ reporter. Also, GFP expression can have subtle effects on eye morphology, with neural and ubiquitous expression. Since GAL4/UAS expression of GFP can influence aging and climbing ability in the Drosophila system of directed gene expression, we found that the latter of these advantages, namely its harmless, non-toxic nature, can be conditional, depending upon the mode of expression and the biological endpoint. We suggest that caution should be used when using GFP to visualize cellular events, especially in long-term assays.

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Section: Discussionmentioning

confidence: 99%

Expression of GFP can influence aging and climbing ability in Drosophila

Mawhinney¹,

Staveley²

2011

Genet. Mol. Res.

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“…In our previous work, we extended the real-time tracking system by exploiting fluorescent reporters for simultaneous tracking of movement and tissue-specific gene expression [4,5]. Since DsRED and GFP are used extensively as reporters for transgene expression in Drosophila and other organisms, methods to correlate movement and behaviors with levels of, and changes in, gene expression in vivo should provide insight into regulatory pathways.…”

Section: Recognizing Courtship Behaviormentioning

confidence: 99%

Finding Behavioral Motifs in Fly Trajectories

Grover¹,

Tavaré²

2009

Communications in Information and Systems

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Abstract. Patterns of locomotor activity of a freely moving organism can help characterize its behavioral phenotypes. To infer behavior from such activity in Drosophila melanogaster, we use a real-time image acquisition system to track the movement of multiple flies in three dimensions. When dealing with fly movement trajectories, we must take into account that similar movement patterns can be expressed in different orientations and speeds. In this paper, we present methods to transform the three-dimensional fly movement trajectories into a space that is translation, rotation, scale and timescale invariant. We then propose an approach motivated by sequence alignment to detect similar movement patterns from fly trajectories in order to infer specific behaviors. We demonstrate the accuracy of the methods and highlight their usefulness in studies aimed at characterizing behavioral phenotypes.

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“…The first two types of methods both typically focus on one specific aspect or measure of behavior, sacrificing complexity for feasibility and power in large sample sizes. More recently, a third class of studies that aims for more comprehensive description and mathematical modeling of complex behaviors has focused on Drosophila flying and walking (Montooth et al 2003;Martin 2004;Budick and Dickinson 2006;Valente et al 2007;Grover et al 2008), but has not yet been extended to thermotolerance and typically does not involve large sample sizes.…”

Section: Introductionmentioning

confidence: 99%

Natural Variation in Drosophila Stressed Locomotion Meets or Exceeds Variation Caused by Hsp70 Mutation: Analysis of Behavior and Performance

et al. 2009

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Thermotolerance involves more than life or death. Investigating the complexity of this trait will aid identification of its genetic contributors. We examined variation in thermally stressed walking behavior and performance in natural Drosophila melanogaster strains and strains mutant for the heat shock protein Hsp70, to determine which aspects of locomotion are affected by heat shock and genotype. We developed software for the large-scale capture, analysis, and visualization of locomotion, and determined: (1) Heat shock and thermal pretreatment significantly and differentially impact fly locomotor behavior and performance. (2) Stressed locomotion traits vary extensively among natural strains. (3) Interactions among treatments, strains, and traits are substantial and often counterintuitive. (4) Hsp70 overexpressing flies are faster and more basally thermoprotected in performance than Hsp70 null flies, but null flies are more unidirectional. (5) Natural variation in most stressed locomotion traits exceeds that caused by Hsp70 mutation, reveals uncoupling between thermoprotection of behavior and performance, and suggests significant genetic variation for trait-specific modifiers of thermotolerance.

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Simultaneous tracking of fly movement and gene expression using GFP

Cited by 23 publications

References 33 publications

Expression of GFP can influence aging and climbing ability in Drosophila

Expression of GFP can influence aging and climbing ability in Drosophila

Finding Behavioral Motifs in Fly Trajectories

Natural Variation in Drosophila Stressed Locomotion Meets or Exceeds Variation Caused by Hsp70 Mutation: Analysis of Behavior and Performance

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