Simultaneous utilization of heterotrophic substrates by Hansenula polymorpha MH30 results in enhanced growth rates

Microbial growth on substitutable substrates: Characterizing the consumer-resource relationship

“…However as expected, when glucose is preferentially utilized the uptake rate is the same as in single substrate growth. These simulations are consistent with the experimental observations for growth on xylose- glycerol and glucose-xylose mixtures (Brinkmann and Babel, 1992). Cybernetic models consider enzyme synthesis to be regulated based on the optimal allocation of key resources which are limited in availability.…”

“…The max for glucose is 0.61 h −1 , while that on xylose is 0.175 h −1 and glycerol 0.27 h −1 . A different pattern of chemostat growth is seen for growth of Hansenula polymorpha on glycerol-xylose mixtures by Brinkmann and Babel (1992). In mixed-substrate growth both substrates continue to be utilized at dilution rates higher than D cr for either substrate.…”

“…Baloo and Ramkrishna (1991) have observed that glucose-xylose mixtures which feature the preferential utilization of glucose in substrate-sufficient batch growth follows this trend during continuous cultivation. Brinkmann and Babel (1992) have observed during growth of Hansenula polymorpha in continuous cultures on glucosexylose and glucose-glycerol mixtures that the utilization of xylose and glycerol ceases at dilution rates a little higher than the critical dilution rate (D cr ) observed for these substrates in single substrate chemostat experiments. The max for glucose is 0.61 h −1 , while that on xylose is 0.175 h −1 and glycerol 0.27 h −1 .…”

Gene Regulation in Continuous Cultures: A Unified Theory for Bacteria and Yeasts

“…This growth pattern has been observed in several other systems (reviewed in Dijkhuizen, 1976, 1982), but the most comprehensive data was obtained in studies of the methylotrophic yeasts, Hansenula polymorpha and Candida boidinii, with mixtures of methanol + glucose (Egli et al, 1980(Egli et al, , 1982a(Egli et al, , 1982b(Egli et al, , 1986. 1 These studies, which were performed with various feed concentrations of glucose and methanol revealed several well-defined trends ( Brinkmann and Babel, 1992). Closed and open symbols show the substrate concentrations during single-and mixed-substrate growth, respectively.…”