Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction
Halil İbrahim Ulusoy,
İpek Nur Yiğit,
Ümmügülsüm Polat
et al.
Abstract:An enrichment and determination method based on liquid chromatographically analysis and cloud point extraction (CPE) has been developed for trace levels of B vitamins (B1, B9 and B12) in the proposed study. Vitamin molecules were drawn into the non-ionic surfactant phase of Polyethylene Glycol (PEG-6000) in the presence of pH 9.0 medium. The surfactant-rich phase separated by centrifugation and then dissolved with 700 µL of ethanol. The obtained ethanol phase was filtered by 0.45-micron filter prior to the H… Show more
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