Simvastatin as an emerging pollutant on non-target aquatic invertebrates: effects on antioxidant-related genes in Daphnia magna

Liu, Sijia; Lin, Jia‐Wei; Ding, Rui; Nie, Xiaoqin

doi:10.1007/s11356-022-19466-7

Cited by 3 publications

(1 citation statement)

References 74 publications

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“…3‐NPA significantly increases the production of ROS in mammalian cells (Silva‐Palacios et al, 2017; Zhang et al, 2014), which in turn induces oxidative damage to cells, which ultimately leads to increased apoptosis (Kang et al, 2018). As a member of the Gpx family, Gpx5 plays a role in cellular antioxidant processes by maybe interacting with other antioxidant substances such as Cat, Sod, Gst, Txn1, and Txnrd1 (Aitken, 1999; Liu et al, 2022; Taylor et al, 2013). Cell membranes are susceptible to biophysical damage, particularly in oxidative environments, such as inflammation caused by bacterial attacks (Duan et al, 2015; Kang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Exploring novel function of Gpx5 antioxidant activity: Assisting epididymal cells secrete functional extracellular vesicles

Li,

Jin,

Chen

et al. 2024

Journal Cellular Physiology

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Glutathione peroxisomal‐5 (Gpx5) promotes the elimination of H2O2 or organic hydrogen peroxide, and plays an important role in the physiological process of resistance to oxidative stress (OS). To directly and better understand the protection of Gpx5 against OS in epididymal cells and sperm, we studied its mechanism of antioxidant protection from multiple aspects. To more directly investigate the role of Gpx5 in combating oxidative damage, we started with epididymal tissue morphology and Gpx5 expression profiles in combination with the mouse epididymal epithelial cell line PC1 (proximal caput 1) expressing recombinant Gpx5. The Gpx5 is highly expressed in adult male epididymal caput, and its protein signal can be detected in the sperm of the whole epididymis. Gpx5 has been shown to alleviate OS damage induced by 3‐Nitropropionic Acid (3‐NPA), including enhancing antioxidant activity, reducing mitochondrial damage, and suppressing cell apoptosis. Gpx5 reduces OS damage in PC1 and maintains the well‐functioning extracellular vesicles (EVs) secreted by PC1, and the additional epididymal EVs play a role in the response of sperm to OS damage, including reducing plasma membrane oxidation and death, and increasing sperm motility and sperm‐egg binding ability. Our study suggests that GPX5 plays an important role as an antioxidant in the antioxidant processes of epididymal cells and sperm, including plasma membrane oxidation, mitochondrial oxidation, apoptosis, sperm motility, and sperm‐egg binding ability.

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