Sindbis virus-induced cytopathic effect in clones of Aedes albopictus (Singh) cells

Sarver, Nava; Stollar, Victor

doi:10.1016/s0042-6822(77)80014-7

Cited by 129 publications

(59 citation statements)

References 19 publications

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“…Representative vertebrate and insect cell lines [Vero (African green monkey), BHK-21 (baby hamster kidney), HEK-293 (human embryonic kidney), NIH 3T3 (mouse fibroblast), duck embryo fibroblast (DEF), A6 (Xenopus laevis), Aedes albopictus (C6/36 and C7/10), Culex tarsalis, and Phlebotomus papatasi (PP-9)] were used to determine the in vitro host range of EILV. SINV, which has a broad in vitro host range, was used as a positive control (12)(13)(14). EILV and SINV infected C. tarsalis, P. papatasi, C6/36, and C7/10 cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Vertebrate hosts include fish, equids, birds, amphibians, reptiles, rodents, pigs, humans, and nonhuman primates (9). Consequently, alphaviruses can be cultured in many vertebrate and insect cell lines (11)(12)(13). In contrast, the distantly related fish alphaviruses, which are not known to have arthropod vectors, exhibit a narrow host range (fish cells only) that is at least partially a result of temperature sensitivity (14)(15)(16).…”

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confidence: 99%

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Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication

Nasar

Palacios

Gorchakov

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

171

189

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Most alphaviruses and many other arboviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, humans, and nonhuman primates. Consequently, they can be propagated in most vertebrate and insect cell cultures. This ability of arboviruses to infect arthropods and vertebrates is usually essential for their maintenance in nature. However, several flaviviruses have recently been described that infect mosquitoes but not vertebrates, although the mechanism of their host restriction has not been determined. Here we describe a unique alphavirus, Eilat virus (EILV), isolated from a pool of Anopheles coustani mosquitoes from the Negev desert of Israel. Phylogenetic analyses placed EILV as a sister to the Western equine encephalitis antigenic complex within the main clade of mosquito-borne alphaviruses. Electron microscopy revealed that, like other alphaviruses, EILV virions were spherical, 70 nm in diameter, and budded from the plasma membrane of mosquito cells in culture. EILV readily infected a variety of insect cells with little overt cytopathic effect. However, in contrast to typical mosquito-borne alphaviruses, EILV could not infect mammalian or avian cell lines, and viral as well as RNA replication could not be detected at 37°C or 28°C. Evolutionarily, these findings suggest that EILV lost its ability to infect vertebrate cells. Thus, EILV seems to be mosquito-specific and represents a previously undescribed complex within the genus Alphavirus. Reverse genetic studies of EILV may facilitate the discovery of determinants of alphavirus host range that mediate disease emergence.evolution | Togavirus

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication

Nasar

Palacios

Gorchakov

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

171

189

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“…Given our current understanding of insect innate immunity, the longstanding observation that host-cell RNA levels are reduced in SINV-infected Ae. albopictus cells has a new importance (Sarver & Stollar, 1977). We have recently reported that mild reduction of host gene expression also occurs in SFVinfected Ae.…”

Section: Virus Suppression Of Immune Signalling In Mosquito Cellsmentioning

confidence: 99%

Advances in dissecting mosquito innate immune responses to arbovirus infection

Atkinson

Attarzadeh-Yazdi

Nash

et al. 2009

Journal of General Virology

108

109

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“…Early morphogenetic studies of alphavirus infection, involving heterogeneous populations of Aedes albopictus cells derived from ground larvae (Singh, 1967), revealed no cytopathology (Gliedman et al, 1975;Igarashi et aL, 1977;Luukkonen et al, 1977). Similar studies performed with cell lines subcloned from these cultures have indicated that different populations of mosquito cells manifest a variety of cytopathic responses during the acute infection phase, yet each recovers to establish persistence in approximately the same time period (Miller & Brown, 1992;Tooker & Kennedy, 1981;Sarver & Stollar, 1977).…”

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confidence: 99%

The distribution of Sindbis virus proteins in mosquito cells as determined by immunofluorescence and immunoelectron microscopy

Miller

Brown

1993

Journal of General Virology

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Two Aedes albopietus (mosquito) subclones, C7-10 and C6/36, were examined by immunofluorescence and immunoelectron microscopy for the distribution of Sindbis virus structural and non-structural proteins. Both the viral glycoproteins, E1 and E2, and the non-structural proteins, nsP1 and nsP2, were found within vesicles and electron-dense, amorphous matrices associated with Sindbis virus infection. The labelling patterns indicated that both replication of viral RNA and production of virus particles were localized within the same structures in the infected cell. The data support previous reports that alphavirus infection is contained within specific structures in the cytoplasm and provide additional evidence that the C6/36 and C7-10 subclones may represent different tissue types in the adult insect.

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Sindbis virus-induced cytopathic effect in clones of Aedes albopictus (Singh) cells

Cited by 129 publications

References 19 publications

Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication

Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication

Advances in dissecting mosquito innate immune responses to arbovirus infection

The distribution of Sindbis virus proteins in mosquito cells as determined by immunofluorescence and immunoelectron microscopy

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