Single and multiple high-risk and low-risk Human Papillomavirus association with cervical lesions of 11,224 women in Jakarta

Abstract

Taken together, identification of these HPV types, especially HPV 16, 18, 45, and 52, and their associated cervical lesions may improve cervical cancer preventive strategies in Indonesia.

Distribution and attribution of high-risk human papillomavirus genotypes in cervical precancerous lesions in China

“…[7][8][9][10][11] This study investigated the prevalence, distribution, and attribution of HR-HPV genotypes among women with cervical precancerous lesions in China to develop an HR-HPV-based screening approach and determine the spectrum of HR-HPV genotypes to be covered. 12,13 Materials and methods…”

The landscape of somatic mutations in Indonesian cervical cancer is predominated by the PI3K pathway

“…In Indonesia, a different HPV type distribution amongst the population as well as in cervical cancer patients was described, especially with a significantly higher prevalence rate of HPV 52 [5,6]. In a recent large, retrospective cohort study from Murdiyarso et al, 11.224 cytology swabs from Jakarta area were typed for HPV, and HPV 52 was the most prevalent HPV type in normal cytology (1%), and the second most common type in SCC (26%) [38]. It is unclear why no AC were included in that study.…”

Esophageal squamous cell carcinomas in a Malaysian cohort show a lack of association with human papillomavirus

“…Using the Anyplex II HPV28 Detection system (Seegene, Seoul, South Korea), 19 high-risk HPV (16,18,26,31,33,35,39,45,51,52,53,56,58,59,66, 68, 69, 73 and 82) and nine low-risk (6,11,40,42,43,44,54, 61 and 70) types were identified with the dual priming oligonucleotide and tagging oligonucleotide cleavage and extension technology. In brief, the multiplex PCR assay was carried out using two primer sets.…”

“…In brief, the multiplex PCR assay was carried out using two primer sets. The first primer set (HPV28 A TOM) detects 14 high-risk HPV (16,18,31,33,35,39,45,51,52,56,58,59, 66, 68) types while the second primer set (HPV28 B TOM) detects 5 high-risk (26,53,69,73,82) and nine low-risk types (6,11,40,42,43,44,54,61,70). Amplification was carried out using the following reaction mixtures: 5 μL of the sample DNA, 5 μL of the first primer set (4 × HPV28 A TOM) or 5 μL of the second primer set (4 × HPV28 B TOM), 5 μL premix (4 × Anyplex PCR master mix consisting of DNA polymerase, uracil-DNA-glycosylase and deoxyribonucleotides) and 5 μL RNase-free water (ultrapure PCR grade).…”