Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies

Impact of Adiposity and Fat Distribution, Rather Than Obesity, on Antibodies as an Illustration of Weight-Loss-Independent Exercise Benefits

“…In addition, acute exercise (and therefore independent of weight lost) has a broad impact on immune functions, including granulocytosis, lymphocytosis (antibody-producing cells) and monocytosis [ 51 ], increased natural killer cells [ 52 ], which are very responsive to acute exercise [ 53 ], increased lymphokine-activated killer cells activity [ 54 ] and enhanced T cell activity [ 55 ]. Importantly, acute exercise might promote a redistribution in lymphocyte subsets [ 56 ] including B cells that produce the antibodies [ 57 , 58 ] and which are affected by obesity [ 59 , 60 ] via diverse pathways including leptin-induced reduction in B cells function [ 61 ] as well. These benefits reverse most of those induced by adiposity described earlier ( Figure 1 ).…”

Extracellular Loops of the Treponema pallidum FadL Orthologs TP0856 and TP0858 Elicit IgG Antibodies and IgG+-Specific B-Cells in the Rabbit Model of Experimental Syphilis

“…However, antigen-specific B-cell populations in later stages of differentiation are difficult to characterize by flow cytometry due to the paucity of B-cell surface markers including immunoglobulin ( 78 ). Because of their propensity for strong Ab responses, in recent years, rabbits have gained favor as a source of MAbs, and the technology for producing rabbit MAbs has become well established ( 79 ). The availability of ECL-specific MAbs would enable characterization of opsonic epitopes at the atomic level.…”

Rabbit Antidiethoxyphosphotyrosine Antibody, Made by Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry

“…The limitation in our 2018 study was that although adducts were detected in cell lysates treated with 1 mM chlorpyrifos oxon, we were unable to detect adducts when live cells were treated with a nonlethal dose of chlorpyrifos oxon. Our reading of the literature − and the experience of G. Xiao suggested that a rabbit monoclonal made by single B cell cloning was likely to have a higher affinity than a mouse monoclonal. The 1C6 antibody reported here fulfills this expectation.…”