Single‐cell analysis identified lung progenitor cells in COVID‐19 patients

Zhao, Zhongyang; Zhao, Yu; Zhou, Yueqing; Wang, Xiaofan; Zhang, Ting; Zuo, Wei

doi:10.1111/cpr.12931

Cited by 25 publications

(34 citation statements)

References 35 publications

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“…The gene expression dataset of bronchoalveolar lavage fluid of COVID‐19 patients shows that there are some similarities in the cytokine and apoptosis genes in the ARDS sequencing results induced by Staphylococcal enterotoxin B [ 39 ]. Single cell RNA sequencing of bronchoalveolar lavage fluid from COVID‐19 patients revealed significant amplification of transmembrane 4 L six family member 1 (TM4SF1)+ and keratin 5 (KRT5)+ lung progenitor cells in severe COVID‐19 patients, suggesting a synergistic role of lung progenitor cells in the prevention and supplementation of alveolar loss in COVID‐19 patients [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Among the selected hub genes, we found that HLA‐G and OCLN genes were reported to be related to COVID‐19 in previous studies. Lung progenitor cells of KLT5 + can differentiate into HOP homeobox (HOPX)+ OCLN+ alveolar barrier cells to supplement alveolar loss after SARS‐CoV‐2 infection, restore epithelial barrier and effectively prevent infiltration of inflammatory cells [ 40 ]. Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

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Comprehensive investigation of RNA‐sequencing dataset reveals the hub genes and molecular mechanisms of coronavirus disease 2019 acute respiratory distress syndrome

Deng

Zeng

et al. 2021

IET Systems Biology

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The goal of this study is to reveal the hub genes and molecular mechanisms of the coronavirus disease 2019 (COVID-19) acute respiratory distress syndrome (ARDS) based on the genome-wide RNA sequencing dataset. The RNA sequencing dataset of COVID-19 ARDS was obtained from GSE163426. A total of 270 differentially expressed genes (DEGs) were identified between COVID-19 ARDS and control group patients. Functional enrichment analysis of DEGs suggests that these DEGs may be involved in the following biological processes: response to cytokine, G-protein coupled receptor activity, ionotropic glutamate receptor signalling pathway and G-protein coupled receptor signalling pathway. By using the weighted correlation network analysis approach to analyse these DEGs, 10 hub DEGs that may play an important role in COVID-19 ARDS were identified. A total of 67 potential COVID-19 ARDS targetted drugs were identified by a complement map analysis. Immune cell infiltration analysis revealed that the levels of T cells CD4 naive, T cells follicular helper, macrophages M1 and eosinophils in COVID-19 ARDS patients were significantly different from those in control group patients. In conclusion, this study identified 10 COVID-19 ARDS-related hub DEGs and numerous potential molecular mechanisms through a comprehensive analysis of the RNA sequencing dataset and also revealed the difference in immune cell infiltration of COVID-19 ARDS. K E Y W O R D S acute respiratory distress syndrome, coronavirus disease 2019, GSE163426, hub genes, molecular mechanism 1 | INTRODUCTION Coronavirus disease 2019 (COVID-19), named by the World Health Organization, refers to pneumonia caused by the 2019 novel coronavirus infection. The International Committee on Classification of Viruses also named this new type of coronavirus 'SARS-CoV-2' (Severe Acute Respiratory Syndrome Coronavirus 2) [1]. At present, there are more than 100 million patients diagnosed with COVID-19 worldwide, and more than 3 million patients have died. There are still 30 million patients with COVID-19. The clinical manifestations of COVID-19-infected pneumonia patients include fever, fatigue, and dry cough, which are its main manifestations [2].Upper respiratory symptoms such as nasal congestion and runny nose are rare, and hypoxia is present [3]. About half of the patients have difficulty breathing more than a week later. In severe cases, it leads to acute respiratory distress syndrome (ARDS), septic shock, metabolic acidosis, and coagulation dysfunction [4,5]. ARDS is also the main cause of death for COVID-19 patients. However, it is still unclear which people are more likely to develop ARDS, and which are the related factors in patients who develop ARDS that lead to death. Therefore, we urgently need a better understanding of COVID-19 ARDS.Wangsheng Deng and Jiaxing Zeng contributed equally to this work.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original ...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comprehensive investigation of RNA‐sequencing dataset reveals the hub genes and molecular mechanisms of coronavirus disease 2019 acute respiratory distress syndrome

Deng

Zeng

et al. 2021

IET Systems Biology

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“…ScRNAseq consists in obtaining gene expression profiling at a single-cell resolution, putting in evidence the different cellular states and molecular dynamics of even the rarer subpopulations. This novel technology has been used successfully in several organs—for example, to study Prominin 1+ liver progenitors [ 114 ], Dach1–downregulated lymphoid progenitors [ 115 ] and KTR5+ lung progenitors in COVID-19 patients [ 116 ]. Within the past few years, an increasing number of research groups have applied this strategy to define the cell populations of the kidneys in mice and humans [ 117 , 118 , 119 , 120 ].…”

Section: Outlook On the Future Of Renal Progenitorsmentioning

confidence: 99%

Molecular Mechanisms of Renal Progenitor Regulation: How Many Pieces in the Puzzle?

Peired

Melica

Molli

et al. 2021

Cells

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Kidneys of mice, rats and humans possess progenitors that maintain daily homeostasis and take part in endogenous regenerative processes following injury, owing to their capacity to proliferate and differentiate. In the glomerular and tubular compartments of the nephron, consistent studies demonstrated that well-characterized, distinct populations of progenitor cells, localized in the parietal epithelium of Bowman capsule and scattered in the proximal and distal tubules, could generate segment-specific cells in physiological conditions and following tissue injury. However, defective or abnormal regenerative responses of these progenitors can contribute to pathologic conditions. The molecular characteristics of renal progenitors have been extensively studied, revealing that numerous classical and evolutionarily conserved pathways, such as Notch or Wnt/β-catenin, play a major role in cell regulation. Others, such as retinoic acid, renin-angiotensin-aldosterone system, TLR2 (Toll-like receptor 2) and leptin, are also important in this process. In this review, we summarize the plethora of molecular mechanisms directing renal progenitor responses during homeostasis and following kidney injury. Finally, we will explore how single-cell RNA sequencing could bring the characterization of renal progenitors to the next level, while knowing their molecular signature is gaining relevance in the clinic.

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“…ScRNAseq consists in obtaining gene expression profiling at a single-cell resolution, putting in evidence the different cellular states and molecular dynamics of even the rarer subpopulations. This novel technology has been used successfully in several organs-for example, to study Prominin 1+ liver progenitors [114], Dach1-downregulated lymphoid progenitors [115] and KTR5+ lung progenitors in COVID-19 patients [116]. Within the past few years, an increasing number of research groups have applied this strategy to define the cell populations of the kidneys in mice and humans [117][118][119][120].…”

Section: Regulators Of Tubular Progenitors In Pathology: a Cacophonus Choirmentioning

confidence: 99%

Molecular Mechanisms of Renal Progenitor Regulation: How Many Pieces in the Puzzle?

Peired¹,

Melica²,

Molli³

et al. 2020

Preprint

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Kidneys of mice, rats and humans possess progenitors that maintain daily homeostasis and take part in endogenous regenerative processes following injury, owing to their capacity to proliferate and differentiate. In the glomerular and tubular compartments of the nephron, consistent studies demonstrated that well-characterized, distinct populations of progenitor cells, localized in the parietal epithelium of Bowman capsule and scattered in the proximal and distal tubules, could generate segment-specific cells in physiological conditions and following tissue injury. However, defective or abnormal regenerative responses of these progenitors can contribute to pathologic conditions. The molecular characteristics of renal progenitors have been extensively studied, revealing that numerous classical and evolutionarily conserved pathways, such as Notch or Wnt/β-catenin, play a major role in cell regulation. Others, such as retinoic acid, renin-angiotensin-aldosterone system, TLR2 (Toll-Like Receptor 2) and leptin, are also important in this process. In this review, we summarize the plethora of molecular mechanisms directing renal progenitor responses during homeostasis and following kidney injury. Finally, we will explore how single cell RNA sequencing could bring the characterization of renal progenitors to the next level, while knowing their molecular signature is gaining relevance in the clinic.

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Single‐cell analysis identified lung progenitor cells in COVID‐19 patients

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 25 publications

References 35 publications

Comprehensive investigation of RNA‐sequencing dataset reveals the hub genes and molecular mechanisms of coronavirus disease 2019 acute respiratory distress syndrome

Comprehensive investigation of RNA‐sequencing dataset reveals the hub genes and molecular mechanisms of coronavirus disease 2019 acute respiratory distress syndrome

Molecular Mechanisms of Renal Progenitor Regulation: How Many Pieces in the Puzzle?

Molecular Mechanisms of Renal Progenitor Regulation: How Many Pieces in the Puzzle?

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