Single Cell Analysis Reveals a New Microsporidia-Host Association in a Freshwater Lake

Chauvet, Marina; Monjot, Arthur; Moné, Anne; Lepère, Cécile

doi:10.1007/s00248-022-02023-7

Cited by 1 publication

(5 citation statements)

References 19 publications

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“…Using TSA‐FISH with a universal microsporidian probe (USP01; Chauvet et al 2022 a ) we confirmed the Microsporidia‐ Kellicottia (rotifer) association already observed in another lake (Chauvet et al 2022 a , b ), here in lake Chauvet. We also identified microsporidian infections in dinoflagellates (probably belonging the genus Ceratium ) both in freshwater and marine ecosystems (Fig.…”

Section: Resultssupporting

confidence: 84%

“…In this genus, only one species is described ( Crispospora chironomi ) and known to parasite chironomid larvae ( Chironomus plumosus ) in freshwater ecosystems (Tokarev et al 2010). Moreover, a recent study suggests that a new Crispospora species might be able to parasitize Kellicottia rotifers in lake environments (Chauvet et al 2022 b ).…”

Section: Resultsmentioning

confidence: 99%

“…Amplification of the V1–V3 region of the microsporidian gene coding for the small sub‐unit (SSU) rRNA was performed using the universal microsporidian V1F and 530R primers set (Zhu et al 1993; Baker et al 1995). Even though other recently developed primers have shown their efficiency (i.e., CM‐V5F CM‐V5R, ~ 200 bp; Trzebny et al 2020), this set of primers amplify a fragment of ~ 400 bp and has shown its efficiency in the detection of aquatic Microsporidia (Dubuffet et al 2021; Chauvet et al 2022 a , b ). Each polymerase chain reaction (PCR) was performed in a total volume of 30 μ L containing 6 μ L of 5× Green GoTaq Flexi buffer, 2.4 μ L of 25 mM MgCl 2 , 0.18 μ L of GoTaqG2 Flexi DNA Polymerase (Promega; 5 U/ μ L), 0.6 μ L of 10 mM each deoxyribonucleotide triphosphates (dNTP), 0.3 μ L of 50 mg mL −1 bovine serum albumin (BSA), and 0.6 μ L of each 10 μ M primer.…”

Section: Methodsmentioning

confidence: 96%

“…In these studies, between 40% and 50% of the uncovered diversity could not be affiliated to a genus suggesting that the microsporidian phylum hide a large amount of undiscovered diversity. These studies also provided temporal variation data and highlighted new microsporidian planktonic hosts in a small size fraction (i.e., < 150 μ m; Chauvet et al 2022 a , b ). Moreover, few Microsporidia are currently described as parasites of protists or microzooplankton (Murareanu et al 2021), even though they are major components of food webs.…”

mentioning

confidence: 92%

“…Altogether, the previous studies did not allow a broad comparison of the diversity of Microsporidia across aquatic ecosystems and thus lead to an incomplete picture of their diversity. Although Dubuffet et al (2021) and Chauvet et al (2022 a , b ) showed an in‐depth description of the microsporidian diversity, these studies were limited to two lakes (Aydat and Pavin, Massif Central, France). Moreover, the microsporidian diversity in marine ecosystems is probably still underestimated as it has been neglected so far.…”

mentioning

confidence: 99%

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High diversity of microsporidian parasites and new planktonic hosts in freshwater and marine ecosystems

Chauvet

Monjot

Lepère

2023

Limnology & Oceanography

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Microsporidia are a large group of obligate intracellular eukaryotic parasites related to Fungi primarily known as parasites of vertebrates and invertebrates. They are well described as parasites of organisms of interest (e.g., edible fish and crustaceans, honeybees, bioindicators such as daphnia, humans) on which they can have an important impact (e.g., reduced survival or fecundity and sex ratio distortion). However, their diversity in aquatic environments, especially in marine ecosystems, has been greatly understudied since they are not targeted by classical eukaryotic primers used in metabarcoding studies. Moreover, little is known about their hosts among protists or microzooplankton and therefore about their impact on the trophic food web functioning. In this work, we sampled 15 different sites across marine and freshwater environments, size-fractioned the samples and used microsporidian specific primers associated with metabarcoding to study the microsporidian diversity (and the associated spatial variation). Co-occurrence networks as well as Tyramide Signal Amplification-Fluorescent In Situ Hybridization were used to link potential hosts (planktonic eukaryotes < 150 μm) and Microsporidia diversity. Our analysis unraveled a large microsporidian diversity which was widely divergent between the two environments studied. In both of them, an important part of this diversity was not affiliated to a genus, suggesting an important reservoir of new microsporidian species and thus new hosts among planktonic eukaryotes. Co-occurrence networks and fluorescence microscopy showed for the first time associations between Microsporidia and dinoflagellates in freshwater and marine environments.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%