Single-cell hydrogel encapsulation for enhanced survival of human marrow stromal cells

Karoubi, Golnaz; Ormiston, Mark L.; Stewart, Duncan J.; Courtman, David W.

doi:10.1016/j.biomaterials.2009.06.035

Cited by 117 publications

(121 citation statements)

References 61 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…1-2%) beyond 12 months [5,12,25,26]. As such, a variety of biomaterials have entered active development with the intent of providing a supportive scaffold that improves both acute cell retention and long-term cell viability [8,13,27].…”

Section: Discussionmentioning

confidence: 99%

“…Given that dimerization between alpha and beta subunits is required for integrin binding to ECM, we profiled the expression of operative integrin proteins that would maintain CSC survival signaling [13,14]. We identified two such integrin dimers are αVβ3 and α5β1, binding partners to fibronectin and fibrinogen-proteins that mediate cell growth and migration [18][19][20].…”

Section: Csc Expression Of Adhesion Moleculesmentioning

confidence: 99%

“…These measures address the challenge to acutely retain injected cells within a vascular organ by reducing mechanical extrusion from the myocardium and clearance through lymphatic or venous drainage [10,11]. But once cells are acutely retained in damaged myocardium, ongoing cell death ensues as the suspended cells have lost vital integrin-dependent attachments to the extracellular matrix (ECM) (12) which reduces stimulation of pro-survival pathways and results in detachment-induced programmed cell death (or anoikis) [13,14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function

Mayfield¹,

Tilokee²,

Latham³

et al. 2014

Biomaterials

Self Cite

113

106

View full text Add to dashboard Cite

Transplantation of ex vivo proliferated cardiac stem cells (CSCs) is an emerging therapy for ischemic cardiomyopathy but outcomes are limited by modest engraftment and poor long-term survival. As such, we explored the effect of single cell microencapsulation to increase CSC engraftment and survival after myocardial injection. Transcript and protein profiling of human atrial appendage sourced CSCs revealed strong expression the pro-survival integrin dimers αVβ3 and α5β1-thus rationalizing the integration of fibronectin and fibrinogen into a supportive intracapsular matrix. Encapsulation maintained CSC viability under hypoxic stress conditions and, when compared to standard suspended CSC, media conditioned by encapsulated CSCs demonstrated superior production of pro-angiogenic/cardioprotective cytokines, angiogenesis and recruitment of circulating angiogenic cells. Intra-myocardial injection of encapsulated CSCs after experimental myocardial infarction favorably affected long-term retention of CSCs, cardiac structure and function. Single cell encapsulation prevents detachment induced cell death while boosting the mechanical retention of CSCs to enhance repair of damaged myocardium.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Csc Expression Of Adhesion Moleculesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function

Mayfield¹,

Tilokee²,

Latham³

et al. 2014

Biomaterials

Self Cite

113

106

View full text Add to dashboard Cite

show abstract

“…As one example, the inclusion of fibronectin derived Arg-Gly-Asp (RGD) to poly-(ethylene glycol) (PEG) hydrogel systems illustrates improved human MSCs cell survival in vitro (Salinas and Anseth, 2008). Human plasma fibronectin and human plasma fibrinogen demonstrate enhanced survival of cells and increased cell metabolic activity in vitro when encapsulating human MSCs in fibronectin or fibrinogen containing hydrogel capsules, most likely due to integrin clustering and activation of extracellular signaling cascades such as the mitogen activated protein kinase cascade (Karoubi et al, 2009 (Baeten and Akassoglou, 2011). MMP-9, synthesized by both macrophages as well as ECs, is present within and at the periphery of infarct within 24 h, thereby creating a significant disturbance to BBB integrity following stroke.…”

Section: Ecm-stem Cell Interactionsmentioning

confidence: 99%

Stroke Repair via Biomimicry of the Subventricular Zone

Matta

Gonzalez

2018

Front. Mater.

View full text Add to dashboard Cite

Stroke is among the leading causes of death and disability worldwide, 85% of which are ischemic. Current stroke therapies are limited by a narrow effective therapeutic time and fail to effectively complete the recovery of the damaged area. Magnetic resonance imaging of the subventricular zone (SVZ) following infarct/stroke has allowed visualization of new axonal connections and projections being formed, while new immature neurons migrate from the SVZ to the peri-infarct area. Such studies suggest that the SVZ is a primary source of regenerative cells for the repair and regeneration of stroke-damaged neurons and tissue. Therefore, the development of tissue engineered scaffolds that serve as a bioreplicative SVZ niche would support the survival of multiple cell types that reside in the SVZ. Essential to replication of the human SVZ microenvironment is the establishment of microvasculature that regulates both the healthy and stroke-injured blood-brain barrier, which is dysregulated poststroke. In order to reproduce this niche, understanding how cells interact in this environment is critical, in particular neural stem cells, endothelial cells, pericytes, ependymal cells, and microglia. Remodeling and repair of the matrix-rich SVZ niche by endogenous reparative mechanisms may then support functional recovery when enhanced by an artificial niche that supports the survival and proliferation of migrating vascular and neuronal cells. Critical considerations to mimic this area include an understanding of resident cell types, delivery method, and the use of biocompatible materials. Controlling stem cell survival, differentiation, and migration are key factors to consider when transplanting stem cells. Here, we discuss the role of the SVZ architecture and resident cells in the promotion and enhancement of endogenous repair mechanisms. We elucidate the interplay between the extracellular matrix composition and cell interactions prior to and following stroke. Finally, we review current cell and neuronal niche biomimetic materials that allow for a tissue-engineered approach in order to promote structural and functional restoration of neural circuitry. By creating an artificial mimetic SVZ, tissue engineers can strive to facilitate tissue regeneration and functional recovery.

show abstract

“…A recent study described the single-cell encapsulation of hMSCs in FN-and fibrinogen-containing hydrogel capsules that rescue the cells from apoptosis induced by loss of anchorage, while providing an increased cell metabolic activity in culture. Effects that were certainly mediated via the MAPK/ERK signalling cascade and upstream integrin/ECM molecule interactions (Karoubi et al, 2009). Using a subpopulation of hMSCs, we recently showed in a rat model of global ischemia, that marrow-isolated adult multilineage inducible (MIAMI) cells (D'Ippolito et al, 2004) adhered onto PLGA microcarriers with a FN biomimetic surface enhanced the neuroprotection effect observed with the cells alone .…”

Section: Molecular Mechanisms Of Cell Adhesion To Scaffoldsmentioning

confidence: 99%

Advances in the Combined Use of Adult Cell Therapy and Scaffolds for Brain Tissue Engineering

Garbayo¹,

Delcroix²,

Schiller³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

View full text Add to dashboard Cite

Single-cell hydrogel encapsulation for enhanced survival of human marrow stromal cells

Cited by 117 publications

References 61 publications

The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function

The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function

Stroke Repair via Biomimicry of the Subventricular Zone

Advances in the Combined Use of Adult Cell Therapy and Scaffolds for Brain Tissue Engineering

Contact Info

Product

Resources

About