Single-cell mechanical analysis and tension quantification via electrodeformation relaxation

Moazzeni, Seyedsajad; Demiryurek, Yasir; Yu, Miao; Shreiber, David I.; Zahn, Jeffrey D.; Shan, Jerry W.; Foty, Ramsey A.; Liu, Liping; Lin, Hao

doi:10.1103/physreve.103.032409

Cited by 12 publications

(3 citation statements)

References 75 publications

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“…1d. Measured tension for briefer aspiration (10-sec hold time) was greater when compared with all other groups, whereas relatively longer aspirations (60-sec hold time) resulted in significantly lower measured values, in agreement with previous findings [36,37].…”

Section: Cortical Tension Quantificationsupporting

confidence: 90%

Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1

Lin,

Moazzeni,

Kyker-Snowman

et al. 2023

Preprint

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Tension-adhesion interplay is a crucial mechanism in multicellular organisms that determines the tension differential among internal and external interfaces, which in turn, mediates tissue surface tension and cell sorting, morphogenesis and remodeling, and cancer progression. Cadherins are widely believed to be involved, yet key aspects of the process are neither well characterized nor quantified. This study demonstrates the critical role of N-cadherin in driving mechanical polarization throughout the actin cortical network. N-cadherin regulates both tension increase at the cell-medium (external) interface and decrease at the cell-cell (internal) interface, and their quantitative magnitudes, both absolute and relative, strongly depend on the surface density of N-cadherin. Furthermore, the strength of polarization also increases with respect to the number of cell-cell interfaces for cells within a multicellular cluster. The cadherin-actin contractility linkage is mediated by Rac1, which serves as a molecular switch to trigger cortex remodeling and contraction via myosin II. Inhibition of Rac1 activity decreases polarization and leads to reduced coherence in both small clusters and spheroids. These results provide a pathway to reconcile opposing theories for tissue surface tension generation and perspectives in cancer treatment.

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Section: Cortical Tension Quantificationsupporting

confidence: 90%

Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1

Lin,

Moazzeni,

Kyker-Snowman

et al. 2023

Preprint

Self Cite

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“…Many studies have been dedicated to establishing various models of biological cells, aiming to study the mechanical properties of cell membranes [114,122,123]. These models provide explanations from different perspectives, encompassing molecular structure, composition, molecular organization, and surface tension.…”

Section: Electroporationmentioning

confidence: 99%

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Li,

Xue,

et al. 2023

Electrophoresis

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Single‐cell biophysical properties play a crucial role in regulating cellular physiological states and functions, demonstrating significant potential in the fields of life sciences and clinical diagnostics. Therefore, over the last few decades, researchers have developed various detection tools to explore the relationship between the biophysical changes of biological cells and human diseases. With the rapid advancement of modern microfabrication technology, microfluidic devices have quickly emerged as a promising platform for single‐cell analysis offering advantages including high‐throughput, exceptional precision, and ease of manipulation. Consequently, this paper provides an overview of the recent advances in microfluidic analysis and detection systems for single‐cell biophysical properties and their applications in the field of cancer. The working principles and latest research progress of single‐cell biophysical property detection are first analyzed, highlighting the significance of electrical and mechanical properties. The development of data acquisition and processing methods for real‐time, high‐throughput, and practical applications are then discussed. Furthermore, the differences in biophysical properties between tumor and normal cells are outlined, illustrating the potential for utilizing single‐cell biophysical properties for tumor cell identification, classification, and drug response assessment. Lastly, we summarize the limitations of existing microfluidic analysis and detection systems in single‐cell biophysical properties, while also pointing out the prospects and future directions of their applications in cancer diagnosis and treatment.

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“…Various methods, such as magnetic twisting cytometry, [18] atomic force microscopy (AFM), [19,20] and microfluidics-based platforms, [21][22][23] have been developed to study cell deformation behaviors using the viscoelastic model. The measurements are usually performed at a short timescale (<1 s) to make cell fulfil the model.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Cancer Cell Mechanics by Measuring Active Deformation Behavior

Feng,

Sun,

Chen

et al. 2023

Small Methods

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Active deformation behavior reflects cell structural dynamics adapting to varying environmental constraints during malignancy progression. In most cases, cell mechanics is characterized by modeling using static equilibrium systems, which fails to comprehend cell deformation behavior leading to inaccuracies in distinguishing cancer cells from normal cells. Here, a method is introduced to measure the active deformation behavior of cancer cells using atomic force microscopy (AFM) and the newly developed deformation behavior cytometry (DBC). During the measurement, cells are deformed and allows a long timescale relaxation (≈5 s). Two parameters are derived to represent deformation behavior: apparent Poisson's ratio for adherent cells, which is measured with AFM and refers to the ratio of the lateral strain to the longitudinal strain of the cell, and shape recovery for suspended cells, which is measured with DBC. Active deformation behavior defines cancer cell mechanics better than traditional mechanical parameters (e.g., stiffness, diffusion, and viscosity). Additionally, aquaporins are essential for promoting the deformation behavior, while the actin cytoskeleton acts as a downstream effector. Therefore, the potential application of the cancer cell active deformation behavior as a biomechanical marker or therapeutic target in cancer treatment should be evaluated.

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Single-cell mechanical analysis and tension quantification via electrodeformation relaxation

Cited by 12 publications

References 75 publications

Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1

Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Characterization of Cancer Cell Mechanics by Measuring Active Deformation Behavior

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