Single-Cell Protein and RNA Expression Analysis of Mononuclear Phagocytes in Intestinal Mucosa and Mesenteric Lymph Nodes of Ulcerative Colitis and Crohn’s Disease Patients

Chapuy, Laurence; Sarfati, Marika

doi:10.3390/cells9040813

Cited by 15 publications

(13 citation statements)

References 75 publications

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“…Some researchers also indicated that all cDC2s should express CLEC10A, a previously defined marker gene for the cDC2 subtype DC2 (6,7). A study based on high-dimensional protein and RNA single-cell analyses revealed the presence of a CD1c low CD14 1 cDC2 subtype and identified another set of surface markers for DC2s and DC3s (4). The heterogeneity of DCs is still unclear, especially considering cDC2s.…”

Section: Single-cell Analysis Reveals the Heterogeneity Of Monocyte-d...mentioning

confidence: 99%

Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral Type-2 Conventional Dendritic Cells

Gao

et al. 2021

The Journal of Immunology

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Section: Single-cell Analysis Reveals the Heterogeneity Of Monocyte-d...mentioning

confidence: 99%

Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral Type-2 Conventional Dendritic Cells

Gao

et al. 2021

The Journal of Immunology

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“…More studies on this topic in patients and mice are needed. Not only bacterial DNA (Kaewduangduen et al, 2022), but LPS andBG (Issara-Amphorn et al, 2018) in serum from dengue-induced leaky gut syndrome also induce proinflammatory effect and more profoundly activate monocytes (Wu et al, 2013) and NK cells (Poggi et al, 2019;Chapuy & Sarfati, 2020) compared with patients without gut barrier defect. Indeed, monocytes are important immune cells that recognize pathogen molecules in several inflammatory diseases in either sepsis (Aguilar-Briseño et al, 2020) or non-sepsis (Grip et al, 2007;Mukherjee et al, 2015;Weldon et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease

Chancharoenthana

Kamolratanakul

Ariyanon

et al. 2022

Front. Cell. Infect. Microbiol.

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Despite a well-known association between gut barrier defect (leaky gut) and several diseases, data on translocation of pathogen molecules, including bacterial DNA (blood bacteriome), lipopolysaccharide (LPS), and serum (1→3)-β-D-glucan (BG), from the gut to the blood circulation (gut translocation) in dengue are still less studied. Perhaps, dengue infection might induce gut translocation of several pathogenic molecules that affect the disease severity. At the enrollment, there were 31 dengue cases in febrile and critical phases at 4.1 ± 0.3 days and 6.4 ± 1.1 days of illness, respectively, with the leaky gut as indicated by positive lactulose-to-mannitol excretion ratio. With blood bacteriome, the patients with critical phase (more severe dengue; n = 23) demonstrated more predominant abundance in Bacteroidetes and Escherichia spp. with the lower Bifidobacteria when compared with the healthy control (n = 5). Meanwhile, most of the blood bacteriome results in dengue with febrile stage (n = 8) were comparable to the control, except for the lower Bifidobacteria in dengue cases. Additionally, endotoxemia at the enrollment was demonstrated in five (62.5%) and 19 (82.6%) patients with febrile and critical phases, respectively, while serum BG was detectable in two (25%) and 20 (87%) patients with febrile and critical phases, respectively. There were higher peripheral blood non-classical monocytes and natural killer cells (NK cells) at the enrollment in patients with febrile phage than in the cases with critical stage. Then, non-classical monocytes (CD14-CD16+) and NK cells (CD56+CD16-) increased at 4 and 7 days of illness in the cases with critical and febrile stages, respectively, the elevation of LPS and/or BG in serum on day 7 was also associated with the increase in monocytes, NK cells, and cytotoxic T cells. In summary, enhanced Proteobacteria (pathogenic bacteria from blood bacteriomes) along with increased endotoxemia and serum BG (leaky gut syndrome) might be collaborated with the impaired microbial control (lower non-classical monocytes and NK cells) in the critical cases and causing more severe disease of dengue infection.

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“…The UC microenvironment is composed of epithelial cells, immune cells, and stromal cells. Recently, scRNA-seq has helped resolve the heterogeneity of the UC microenvironment among different patients at the transcriptional level 16,17,37 . However, single-cell spatial heterogeneity of the UC microenvironment remains to be deciphered.…”

Section: Discussionmentioning

confidence: 99%

Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis

Zhang

Cao

et al. 2021

Preprint

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Background and Aims: Ulcerative colitis(UC) is a chronic inflammatory bowel disorder with highly cellular heterogeneity. Mass cytometry(Cy-TOF) and single-cell RNA sequencing (scRNA-seq) have revealed cellular heterogeneity of UC. However, comprehensive elucidation of tissue topological changes within the UC ecosystem is still missing. And we aimed to illustrate compositional and spatial changes of the UC ecosystem.Methods: Imaging mass cytometry (IMC) and scRNA-seq were applied to depict the single-cell landscape of colon ecosystem.Results: We noticed tissue topological changes featured with macrophage disappearance reaction (MDR) in UC region. MDR only occurred for CD163+ tissue-resident macrophages. We found reactive oxygen species (ROS) level were higher in UC region but ROS scavenging enzyme SOD1/2 were barely detected in resident macrophages, resulting selective oxidative protection for inflammatory macrophages and resident macrophage disappearance reaction. Furthermore, inflammatory macrophages replaced resident macrophages during UC, which played a key role in forming the inflammatory cellular network by producing TNF-α and IL-1β. Conclusions: Our study dissected the microenvironment of UC lesions at single-cell resolution while preserving its architecture, based on which, we discovered the mechanism of MDR in UC region and resident macrophage specific MDR resulted in infiltration of inflammatory macrophage, which formed the cytokine producing network within the local cellular neighborhood.

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Single-Cell Protein and RNA Expression Analysis of Mononuclear Phagocytes in Intestinal Mucosa and Mesenteric Lymph Nodes of Ulcerative Colitis and Crohn’s Disease Patients

Cited by 15 publications

References 75 publications

Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral Type-2 Conventional Dendritic Cells

Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral Type-2 Conventional Dendritic Cells

Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease

Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis

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