Single-cell Raman micro-spectroscopy for tracking of carotenoids in cyanobacteria exposed to Mn and Zn doped ferrite nanoparticles

Nekvapil, Fran; Bunge, Alexander; Tudoran, Lucian Barbu; Pinzaru, Simona Cîntă

doi:10.1016/j.saa.2021.119607

Cited by 15 publications

(6 citation statements)

References 31 publications

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“…Utilizing Raman microspectroscopy, visualization of NPs transmembrane transport into single cells has been achieved for magnetic NPs, [ 282 ] metallacarborane aggregates, [ 283 ] and carbon NPs. [ 284 ] Dorney et al.…”

Section: Imaging‐based Np‐tracing Techniques In Intracellular Deliverymentioning

confidence: 99%

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Zhou,

Liu,

Wang

et al. 2024

Advanced Science

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Intracellular delivery of nano‐drug‐carriers (NDC) to specific cells, diseased regions, or solid tumors has entered the era of precision medicine that requires systematic knowledge of nano‐biological interactions from multidisciplinary perspectives. To this end, this review first provides an overview of membrane‐disruption methods such as electroporation, sonoporation, photoporation, microfluidic delivery, and microinjection with the merits of high‐throughput and enhanced efficiency for in vitro NDC delivery. The impact of NDC characteristics including particle size, shape, charge, hydrophobicity, and elasticity on cellular uptake are elaborated and several types of NDC systems aiming for hierarchical targeting and delivery in vivo are reviewed. Emerging in vitro or ex vivo human/animal‐derived pathophysiological models are further explored and highly recommended for use in NDC studies since they might mimic in vivo delivery features and fill the translational gaps from animals to humans. The exploration of modern microscopy techniques for precise nanoparticle (NP) tracking at the cellular, organ, and organismal levels informs the tailored development of NDCs for in vivo application and clinical translation. Overall, the review integrates the latest insights into smart nanosystem engineering, physiological models, imaging‐based validation tools, all directed towards enhancing the precise and efficient intracellular delivery of NDCs.

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Section: Imaging‐based Np‐tracing Techniques In Intracellular Deliverymentioning

confidence: 99%

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Zhou,

Liu,

Wang

et al. 2024

Advanced Science

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“…Se sabe que el género Synechocystis de Cianobacterias (Fig. 92) contiene niveles más altos de β-caroteno y otros carotenoides que contienen 9 enlaces dobles carbono-carbono en su cadena de polieno (9 carotenoides C=C), como se reveló previamente mediante métodos de espectroscopia Raman de resonancia, HPLC y biología molecular (Nekvapil et al, 2021). Debido a la creciente necesidad de sostenibilidad, los carotenoides microbianos se están convirtiendo en un sustituto prometedor de los producidos por síntesis química y extracción de pigmentos vegetales debido a su compatibilidad con el medio ambiente (Peng et al, 2021).…”

Section: Filo Cyanobacteriaunclassified

“…(A) Vista transversal de la célula Synechocystis con pili. (B) Ultraestructura de la célula de Synechocystis(Nekvapil et al, 2021).…”

unclassified

Carotenoides

Romero,

Espinoza

2024

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Con gran satisfacción y entusiasmo se presenta ante la comunidad científica, la presente obra, un libro dedicado a despejar las innumerables dudas respecto al conocimiento de las características, propiedades y métodos de extracción de carotenoides: carotenos y xantófilas. Este libro enfatiza en la importancia que tiene conocer acerca de la estructura, propiedades, biosíntesis, biodisponibilidad en el cuerpo humano, factores que afectan su biodisponibilidad y técnicas de extracción de los carotenoides es para conocer el fundamento de sus funciones esenciales tanto en las plantas como en el ser vivo, en plantas, captan la energía luminosa y actúan como antioxidantes. En el ser vivo al consumir los carotenoides se transforman en vitamina A, micronutriente que interviene en una amplia variedad de funciones fisiológicas, entre las que destaca la función visual, formación y mantenimiento de tejidos blandos, dientes, mucosas y piel, y tiene también un papel clave en el desarrollo fetal, embarazo y lactancia.

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“…Raman spectroscopy was used to determine the carotenoid profile in snow algae and cyanobacteria (Osterrothová et al ., 2019; Nekvapil et al ., 2021). Both works show promising results to quantify carotenoids, although the method was applied for single‐cell analyses and not as a cultivation sensor.…”

Section: Case‐study: Microalgae Productionmentioning

confidence: 99%

Perspectives of fluorescence spectroscopy for online monitoring in microalgae industry

Sá

Ferrer-Ledo

Gao

et al. 2022

Microbial Biotechnology

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Summary Microalgae industrial production is viewed as a solution for alternative production of nutraceuticals, cosmetics, biofertilizers, and biopolymers. Throughout the years, several technological advances have been implemented, increasing the competitiveness of microalgae industry. However, online monitoring and real‐time process control of a microalgae production factory still require further development. In this mini‐review, non‐destructive tools for online monitoring of cellular agriculture applications are described. Still, the focus is on the use of fluorescence spectroscopy to monitor several parameters (cell concentration, pigments, and lipids) in the microalgae industry. The development presented makes it the most promising solution for monitoring up‐and downstream processes, different biological parameters simultaneously, and different microalgae species. The improvements needed for industrial application of this technology are also discussed.

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Single-cell Raman micro-spectroscopy for tracking of carotenoids in cyanobacteria exposed to Mn and Zn doped ferrite nanoparticles

Cited by 15 publications

References 31 publications

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Carotenoides

Perspectives of fluorescence spectroscopy for online monitoring in microalgae industry

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