Single-Cell RNA Sequencing Identifies Yes-Associated Protein 1–Dependent Hepatic Mesothelial Progenitors in Fibrolamellar Carcinoma

Cellular origins of regenerating liver and hepatocellular carcinoma

“… 128 , 129 In contrast, the molecular profile of patient-derived xenografts has implicated biliary tree stem cells located in peribiliary glands or hepatic mesothelial progenitors as potential cells of origin for FLC. 130 , 131 As shown with HCC, a variety of hepatic cell types may have the capacity to establish FLC once the oncogenic fusion kinase is expressed in cells. However, it may be difficult to develop lineage tracing models to define the usual cell type of origin as FLC develops without chronic liver injury.…”

AKAP12 ameliorates liver injury via targeting PI3K/AKT/PCSK6 pathway

“…AKAP12 acts as a regulator of mitosis by anchoring key signaling proteins, such as PKA, PKC, and cyclins, thereby inhibiting the viability of tumor cells and inducing apoptosis via caspase-3 [ 4 , 9 , 10 ]. Notably, AKAP12 is reportedly downregulated in hepatocellular carcinoma (HCC) via promoter hypermethylation [ [11] , [12] , [13] , [14] ] and reduced in hepatic cirrhosis and precancerous lesions via upregulation of microRNA (miR)-183 and miR-186, which both target AKAP12. In addition, miR-1251-5p negatively regulates AKAP12 expression and plays a carcinogenic role in HCC [ 12 ].…”

Chemical, molecular, and single cell analysis reveal chondroitin sulfate proteoglycan aberrancy in fibrolamellar carcinoma

“…Another major component of this study is the first-ever single-cell ATAC-seq analysis of FLC. All prior genome-scale analyses of FLC have been performed on bulk tumors and the only published single-cell analysis related to this cancer involves a patient-derived xenograft (PDX), not a primary tumor (47) . Our study overcomes these limitations and provides the first glimpse into FLC tumor tissue complexity.…”