Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives

Jia, Hanbo; Wang, Wei; Zhou, Zhaowen; Chen, Zhiyi; Lan, Zijun; Bo, Hao; Fan, Liqing

doi:10.1007/s11010-023-04840-x

Cited by 5 publications

(2 citation statements)

References 138 publications

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“…As these cells progress through various stages of maturation, they may shed RNAs into the seminal plasma. Like cfDNA, apoptosis of germ cells and somatic cells within the testes can lead to the release of RNAs into seminal plasma ( 62 ). In addition, prostate and seminal vesicles can release RNA molecules in the seminal plasma, thus reflecting the physiological and pathological state of these tissues ( 63 ).…”

Section: Potential Non-invasive Biomarkers In Noamentioning

confidence: 99%

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Fontana,

Sirchia,

Pesenti

et al. 2024

Front. Endocrinol.

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Recent advancements in reproductive medicine have guided novel strategies for addressing male infertility, particularly in cases of non-obstructive azoospermia (NOA). Two prominent invasive interventions, namely testicular sperm extraction (TESE) and microdissection TESE (micro-TESE), have emerged as key techniques to retrieve gametes for assisted reproduction technologies (ART). Both heterogeneity and complexity of NOA pose a multifaceted challenge to clinicians, as the invasiveness of these procedures and their unpredictable success underscore the need for more precise guidance. Seminal plasma can be aptly regarded as a liquid biopsy of the male reproductive tract, encompassing secretions from the testes, epididymides, seminal vesicles, bulbourethral glands, and prostate. This fluid harbors a variety of cell-free nucleic acids, microvesicles, proteins, and metabolites intricately linked to gonadal activity. However, despite numerous investigations exploring potential biomarkers from seminal fluid, their widespread inclusion into the clinical practice remains limited. This could be partially due to the complex interplay of diverse clinical and genetic factors inherent to NOA that likely contributes to the absence of definitive biomarkers for residual spermatogenesis. It is conceivable that the integration of clinical data with biomarkers could increase the potential in predicting surgical procedure outcomes and their choice in NOA cases. This comprehensive review addresses the challenge of sperm retrieval in NOA through non-invasive biomarkers. Moreover, we delve into promising perspectives, elucidating innovative approaches grounded in multi-omics methodologies, including genomics, transcriptomics and proteomics. These cutting-edge techniques, combined with the clinical and genetics features of patients, could improve the use of biomarkers in personalized medical approaches, patient counseling, and the decision-making continuum. Finally, Artificial intelligence (AI) holds significant potential in the realm of combining biomarkers and clinical data, also in the context of identifying non-invasive biomarkers for sperm retrieval.

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Section: Potential Non-invasive Biomarkers In Noamentioning

confidence: 99%

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Fontana,

Sirchia,

Pesenti

et al. 2024

Front. Endocrinol.

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show abstract

“…The emergence of high-throughput single-cell RNA sequencing (scRNA-Seq) and single-nucleus RNA-Seq (snRNA-Seq) analyses has introduced a new era in male reproductive research [ 6 , 7 ]. Bypassing the intricate nature of testicular tissues, these techniques facilitate the detection of uncommon and heterogeneous cell populations at the single-cell level [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Single-Nucleus RNA-Seq Reveals Spermatogonial Stem Cell Developmental Pattern in Shaziling Pigs

Tang,

Chen,

Yan

et al. 2024

Biomolecules

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Normal testicular development ensures the process of spermatogenesis, which is a complex biological process. The sustained high productivity of spermatogenesis throughout life is predominantly attributable to the constant proliferation and differentiation of spermatogonial stem cells (SSCs). The self-renewal and differentiation processes of SSCs are strictly regulated by the SSC niche. Therefore, understanding the developmental pattern of SSCs is crucial for spermatogenesis. The Shaziling pig is a medium-sized indigenous pig breed originating from central China. It is renowned for its superior meat quality and early male sexual maturity. The spermatogenic ability of the boars is of great economic importance to the pig industry. To investigate testicular development, particularly the pattern of SSC development in Shaziling pigs, we used single-cell transcriptomics to identify gene expression patterns in 82,027 individual cells from nine Shaziling pig testes at three key postnatal developmental stages. We generated an unbiased cell developmental atlas of Shaziling pig testicular tissues. We elucidated the complex processes involved in the development of SSCs within their niche in the Shaziling pig. Specifically, we identified potential marker genes and cellular signaling pathways that regulate SSC self-renewal and maintenance. Additionally, we proposed potential novel marker genes for SSCs that could be used for SSC isolation and sorting in Shaziling pigs. Furthermore, by immunofluorescence staining of testicular tissues of different developmental ages using marker proteins (UCHL1 and KIT), the developmental pattern of the spermatogonia of Shaziling pigs was intensively studied. Our research enhances the comprehension of the development of SSCs and provides a valuable reference for breeding Shaziling pigs.

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Transcriptome sequencing reveals the characteristics of spermatogenesis and testis development in Amphioctopus fangsiao

Jiang,

Sun,

Huang

et al. 2024

Aquaculture Reports

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Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives

Cited by 5 publications

References 138 publications

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Single-Nucleus RNA-Seq Reveals Spermatogonial Stem Cell Developmental Pattern in Shaziling Pigs

Transcriptome sequencing reveals the characteristics of spermatogenesis and testis development in Amphioctopus fangsiao

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