Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis

Han, Yingying; Wang, Dan; Peng, Lushan; Huang, Tao; He, Xiaoyun; Wang, Junpu; Ou, Chunlin

doi:10.1186/s13045-022-01280-w

Cited by 56 publications

(45 citation statements)

References 166 publications

(198 reference statements)

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“…The occurrence of metastasis is influenced not only by the driving force of the tumor itself but also by the environment surrounding the tumor cells. In the TME, TAMs are closely associated with tumor metastasis [ 69 ].…”

Section: Mechanisms Of Tumor Progression Mediated By Macrophagesmentioning

confidence: 99%

Targeting macrophages: a novel treatment strategy in solid tumors

Liu

Song

et al. 2022

J Transl Med

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In the tumor microenvironment (TME), tumor-associated macrophages (TAMs) are the most abundant immune cells, which act as a key regulator in tumorigenesis and progression. Increasing evidence have demonstrated that the TME alters the nature of macrophages to maintain dynamic tissue homeostasis, allowing TAMs to acquire the ability to stimulate angiogenesis, promote tumor metastasis and recurrence, and suppress anti-tumor immune responses. Furthermore, tumors with high TAM infiltration have poor prognoses and are resistant to treatment. In the field of solid tumor, the exploration of tumor-promoting mechanisms of TAMs has attracted much attention and targeting TAMs has emerged as a promising immunotherapeutic strategy. Currently, the most common therapeutic options for targeting TAMs are as follows: the deletion of TAMs, the inhibition of TAMs recruitment, the release of phagocytosis by TAMs, and the reprogramming of macrophages to remodel their anti-tumor capacity. Promisingly, the study of chimeric antigen receptor macrophages (CAR-Ms) may provide even greater benefit for patients with solid tumors. In this review, we discuss how TAMs promote the progression of solid tumors as well as summarize emerging immunotherapeutic strategies that targeting macrophages.

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Section: Mechanisms Of Tumor Progression Mediated By Macrophagesmentioning

confidence: 99%

Targeting macrophages: a novel treatment strategy in solid tumors

Liu

Song

et al. 2022

J Transl Med

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“…Tumor immunotherapy has been widely investigated and employed in clinical fields, as it holds great promise as a neoadjuvant therapy besides surgery and radiotherapy (61)(62)(63). At present, many inhibitors have been identified to play emerging roles in tumor suppression by regulating TME or assisting immuno-surveillance by immune cells (64).…”

Section: Discussionmentioning

confidence: 99%

The Hippo Pathway Effector Transcriptional Co-activator With PDZ-Binding Motif Correlates With Clinical Prognosis and Immune Infiltration in Colorectal Cancer

Wang

Nie²,

Li³

et al. 2022

Front. Med.

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The transcriptional co-activator with PDZ-binding motif (TAZ) is a downstream effector of the Hippo pathway. It has been identified as an oncogene in certain tumor types; however, the function and role of TAZ in colorectal cancer (CRC) has not been illustrated. Here, we aimed to analyze the expression and role of TAZ in CRC. In this study, we investigated the expression level of TAZ in 127 CRC and matched adjacent normal tissues by immunohistochemistry (IHC) and analyzed its correlation with clinicopathological characteristics in CRC. Moreover, we further analyzed the role of TAZ in the CRC-associated immunology using integrative bioinformatic analyses. The cBioPortal and WebGestalt database were used to analyze the co-expressed genes and related pathways of TAZ in CRC by gene ontology (GO) and KEGG enrichment analyses. Meanwhile, the correlations between TAZ and the infiltrating immune cells and gene markers were analyzed by TIMER database. Our study revealed that TAZ expression is higher in CRC tissues than in matched adjacent non-tumor tissues. In addition, CRC patients with higher TAZ expression demonstrated poor overall survival (OS) and recurrent-free survival rates as compared to CRC patients with lower expression of TAZ. Furthermore, the TAZ expression was identified to closely associate with the immune infiltration of CD4 + T, CD8 + T, and B cells. Taken together, our findings suggest that TAZ may serve as a promising prognostic biomarker and therapeutic target in CRC.

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“…As a recent example, when pairs of primary and metastatic tumor tissues were collected from TN patients and evaluated to determine their gene expression, gene mutations were rarely observed, but the gene expression signatures of immunomodulatory and other genes changed; this shift is thought to be one of the causes of the reduced or lack of efficacy of immunotherapeutic agents [ 66 ]. High-resolution profiling of cancer tissue at a single-cell level using scRNA-seq is a powerful tool to elucidate the nature and characteristics of the cell populations in complex types of cancer [ 67 , 68 ]; however, this method does not provide positional information about which cells a cancer cell is interacting with when the tissue is broken down into individual cells for sequencing.…”

Section: Spatial Transcriptomicsmentioning

confidence: 99%

“…Gene expression patterns acquired at the single-cell level and spatial transcriptomes have revealed heterogeneity that exists within breast cancer tissues of patients [ 65 , 67 , 68 , 77 , 79 , 80 ]. In order to utilize and further expand these findings, it is important to improve the accuracy by combining them with other modalities.…”

Section: Ai-based Analysis Of Spatial Transcriptomics and Medical Imagesmentioning

confidence: 99%

Understanding Breast Cancers through Spatial and High-Resolution Visualization Using Imaging Technologies

Takahashi

Kawahara

Kikuchi

2022

Cancers

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Breast cancer is the most common cancer affecting women worldwide. Although many analyses and treatments have traditionally targeted the breast cancer cells themselves, recent studies have focused on investigating entire cancer tissues, including breast cancer cells. To understand the structure of breast cancer tissues, including breast cancer cells, it is necessary to investigate the three-dimensional location of the cells and/or proteins comprising the tissues and to clarify the relationship between the three-dimensional structure and malignant transformation or metastasis of breast cancers. In this review, we aim to summarize the methods for analyzing the three-dimensional structure of breast cancer tissue, paying particular attention to the recent technological advances in the combination of the tissue-clearing method and optical three-dimensional imaging. We also aimed to identify the latest methods for exploring the relationship between the three-dimensional cell arrangement in breast cancer tissues and the gene expression of each cell. Finally, we aimed to describe the three-dimensional imaging features of breast cancer tissues using noninvasive photoacoustic imaging methods.

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Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis

Cited by 56 publications

References 166 publications

Targeting macrophages: a novel treatment strategy in solid tumors

Targeting macrophages: a novel treatment strategy in solid tumors

The Hippo Pathway Effector Transcriptional Co-activator With PDZ-Binding Motif Correlates With Clinical Prognosis and Immune Infiltration in Colorectal Cancer

Understanding Breast Cancers through Spatial and High-Resolution Visualization Using Imaging Technologies

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