Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction

Zhang, Ning; Wang, Yinuo; Chen, Ziyu; Ren, Jianhong; Rehman, Abdur; Ahmad, Dawood Warraich; Long, Deyu; Hou, Junyao; Zhou, Yuwen; Yang, Luyu; Ni, Yu; Li, Yayu; Du, Changjian; Yang, Yuanzheng; Liao, Mingzhi

doi:10.1016/j.biopha.2021.112449

Cited by 12 publications

(7 citation statements)

References 56 publications

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“…Zhang and colleagues performed single-cell RNA sequencing (scRNA-seq) analysis of human juvenile testis determining germ cell-somatic cell interactions. They identified maturation markers expressed in Sertoli cells that regulate sperm maturation and reported that BPA affected the testis microenvironment that contributes to germ cell maturation, as a possible mechanism of BPA reproductive toxicity mechanisms ( 99 ).…”

Section: Consequences Of Exposing Sertoli Cells To Endocrine Disrupti...mentioning

confidence: 99%

Impact of endocrine disrupting chemicals and pharmaceuticals on Sertoli cell development and functions

Corpuz-Hilsabeck

Culty

2023

Front. Endocrinol.

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Sertoli cells play essential roles in male reproduction, from supporting fetal testis development to nurturing male germ cells from fetal life to adulthood. Dysregulating Sertoli cell functions can have lifelong adverse effects by jeopardizing early processes such as testis organogenesis, and long-lasting processes such as spermatogenesis. Exposure to endocrine disrupting chemicals (EDCs) is recognized as contributing to the rising incidence of male reproductive disorders and decreasing sperm counts and quality in humans. Some drugs also act as endocrine disruptors by exerting off-target effects on endocrine tissues. However, the mechanisms of toxicity of these compounds on male reproduction at doses compatible with human exposure are still not fully resolved, especially in the case of mixtures, which remain understudied. This review presents first an overview of the mechanisms regulating Sertoli cell development, maintenance, and functions, and then surveys what is known on the impact of EDCs and drugs on immature Sertoli cells, including individual compounds and mixtures, and pinpointing at knowledge gaps. Performing more studies on the impact of mixtures of EDCs and drugs at all ages is crucial to fully understand the adverse outcomes these chemicals may induce on the reproductive system.

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Section: Consequences Of Exposing Sertoli Cells To Endocrine Disrupti...mentioning

confidence: 99%

Impact of endocrine disrupting chemicals and pharmaceuticals on Sertoli cell development and functions

Corpuz-Hilsabeck

Culty

2023

Front. Endocrinol.

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“…This study found that BPA leads to a dysregulation of secreted protein expression in Sertoli cells during spermatogenesis, which caused cytotoxicity to Sertoli cells [13]. BPA was also shown to be associated with nonobstructive azoospermia [13]. A study by Castellini et al [18] confirmed that BPA has negative impacts on male reproduction, and compares it to two possible industry alternatives: Bisphenol F (BPF) and bisphenol S (BPS).…”

Section: Endocrine-disrupting Chemicals and Other Toxins In Water And...mentioning

confidence: 88%

“…A study by Zhang et al [13] looked at the impact of BPA on male fertility. This study found that BPA leads to a dysregulation of secreted protein expression in Sertoli cells during spermatogenesis, which caused cytotoxicity to Sertoli cells [13]. BPA was also shown to be associated with nonobstructive azoospermia [13].…”

Section: Endocrine-disrupting Chemicals and Other Toxins In Water And...mentioning

confidence: 99%

The impact of air pollution and endocrine disruptors on reproduction and assisted reproduction

Seli

Taylor

2023

Current Opinion in Obstetrics &Amp; Gynecology

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Purpose of reviewRapid increase in world population accompanied by global industrialization has led to an increase in deployment of natural resources, resulting in growing levels of pollution. Here, we review recent literature on the impact of environmental pollution on human reproductive health and assisted reproduction outcomes, focusing on two of the most common: air pollution and endocrine disruptors. Recent findingsAir pollution has been associated with diminished ovarian reserve, uterine leiomyoma, decreased sperm concentration and motility. Air pollution also correlates with decreased pregnancy rates in patients undergoing infertility treatment using in-vitro fertilization (IVF). Similarly, Bisphenol A (BPA), a well studied endocrine disrupting chemical, with oestrogen-like activity, is associated with diminished ovarian reserve, and abnormal semen parameters, while clinical implications for patients undergoing infertility treatment remain to be established. SummaryThere is convincing evidence that environmental pollutants may have a negative impact on human health and reproductive potential. Air pollutions and endocrine disrupting chemicals found in water and food seem to affect male and female reproductive function. Large-scale studies are needed to determine the threshold values for health impact that may drive targeted policies.

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“…Studies have shown that BPA blocks the estrogen response by competing with E2 [ 1 ]. BPA also binds to androgen receptors, leading to androgen-dependent gene regulation disorders [ 2 ] and causing reproductive, developmental, and metabolic diseases [ 1 , 3 , 4 , 5 ]. BPA is carcinogenic and mutagenic, inducing prostate cancer [ 6 ], breast tumors [ 7 ], and ovarian cancer [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of BPA Degradation and Toxicity Resistance in Rhodococcus equi

Tian

Qiu

et al. 2022

Microorganisms

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Bisphenol A (BPA) pollution poses an increasingly serious problem. BPA has been detected in a variety of environmental media and human tissues. Microbial degradation is an effective method of environmental BPA remediation. However, BPA is also biotoxic to microorganisms. In this study, Rhodococcus equi DSSKP-R-001 (R-001) was used to degrade BPA, and the effects of BPA on the growth metabolism, gene expression patterns, and toxicity-resistance mechanisms of Rhodococcus equi were analyzed. The results showed that R-001 degraded 51.2% of 5 mg/L BPA and that 40 mg/L BPA was the maximum BPA concentration tolerated by strain R-001. Cytochrome P450 monooxygenase and multicopper oxidases played key roles in BPA degradation. However, BPA was toxic to strain R-001, exhibiting nonlinear inhibitory effects on the growth and metabolism of this bacterium. R-001 bacterial biomass, total protein content, and ATP content exhibited V-shaped trends as BPA concentration increased. The toxic effects of BPA included the downregulation of R-001 genes related to glycolysis/gluconeogenesis, pentose phosphate metabolism, and glyoxylate and dicarboxylate metabolism. Genes involved in aspects of the BPA-resistance response, such as base excision repair, osmoprotectant transport, iron-complex transport, and some energy metabolisms, were upregulated to mitigate the loss of energy associated with BPA exposure. This study helped to clarify the bacterial mechanisms involved in BPA biodegradation and toxicity resistance, and our results provide a theoretical basis for the application of strain R-001 in BPA pollution treatments.

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Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction

Cited by 12 publications

References 56 publications

Impact of endocrine disrupting chemicals and pharmaceuticals on Sertoli cell development and functions

Impact of endocrine disrupting chemicals and pharmaceuticals on Sertoli cell development and functions

The impact of air pollution and endocrine disruptors on reproduction and assisted reproduction

Mechanisms of BPA Degradation and Toxicity Resistance in Rhodococcus equi

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