Single-cell transcriptomic analysis of corneal organoids during development

Swarup, Aditi; Phansalkar, Ragini; Morri, Maurizio; Agarwal, Aditi; Subramaniam, Varun; Li, BaoXiang; Wu, Albert Y.

doi:10.1016/j.stemcr.2023.10.022

Cited by 7 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study identified MKI67, survivin (BIRC5) and H2A histone family member X (H2AFX)-positive cells with differential expression of CD109 as highly proliferative TACs in human corneas, which had exclusive expression of cyclin-dependent kinase 2 (CKS2), stathmin-1 (STMN1), and UBE2C, which can be taken as their markers [107]. Finally, scRNAseq analysis of human iPSC-derived corneal organoids at 1, 2, 3, and 4 months of development identified that early TACs express CENPF, UBE2C, and NUSAP1 [108]. Many of these proposed markers that are based on scRNAseq analysis still need to be validated in different species and using combinatorial approaches in order for the research community to reach a consensus on reliable, exclusive, and universal markers of TACs and LESCs.…”

Section: Markers Of Corneal Tacsmentioning

confidence: 99%

The Potential Reversible Transition between Stem Cells and Transient-Amplifying Cells: The Limbal Epithelial Stem Cell Perspective

Verma,

Lin,

Coulson-Thomas

2024

Cells

View full text Add to dashboard Cite

Stem cells (SCs) undergo asymmetric division, producing transit-amplifying cells (TACs) with increased proliferative potential that move into tissues and ultimately differentiate into a specialized cell type. Thus, TACs represent an intermediary state between stem cells and differentiated cells. In the cornea, a population of stem cells resides in the limbal region, named the limbal epithelial stem cells (LESCs). As LESCs proliferate, they generate TACs that move centripetally into the cornea and differentiate into corneal epithelial cells. Upon limbal injury, research suggests a population of progenitor-like cells that exists within the cornea can move centrifugally into the limbus, where they dedifferentiate into LESCs. Herein, we summarize recent advances made in understanding the mechanism that governs the differentiation of LESCs into TACs, and thereafter, into corneal epithelial cells. We also outline the evidence in support of the existence of progenitor-like cells in the cornea and whether TACs could represent a population of cells with progenitor-like capabilities within the cornea. Furthermore, to gain further insights into the dynamics of TACs in the cornea, we outline the most recent findings in other organ systems that support the hypothesis that TACs can dedifferentiate into SCs.

show abstract

Section: Markers Of Corneal Tacsmentioning

confidence: 99%