Single cell trapping by capillary pumping using NOA81 replica moulded stencils

Moonen, Emma J.M.; Lüttge, Regina; Frimat, Jean-Philippe

doi:10.1016/j.mee.2018.04.010

Cited by 15 publications

(18 citation statements)

References 17 publications

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“…NOA81 (Norland Optical Adhesive 81) microsieves were fabricated by a double replica molding procedure fully described by Moonen et al [ 31 ]. In brief, the microsieve pattern of an original micro-Silicon Electrode Array (µSEA) fabricated previously by Schurink during his PhD work at University of Twente [ 33 ] was transferred into polydimethylsiloxane (PDMS) using standard soft lithography and NOA81 replica molding.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, fabrication of 3D multilayers patterns for neuronal culture using photo curable materials has rapidly received attention [ 29 , 30 ]. Therefore, lately, we also demonstrated a microsieve structure that was similar to that developed by Schurink et al but was made from a photo-curable optical adhesive (NOA81), and it showed an even higher cell survival than silicon [ 31 ]. Microsieves made from polymer materials, specifically using NOA81, known as an optical adhesive, offer new capabilities in this field of application.…”

Section: Introductionmentioning

confidence: 99%

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Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Sabahi-Kaviani

Lüttge

2020

Micromachines

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We studied the micropattern fidelity of a Norland Optical Adhesive 81 (NOA81) microsieve made by soft-lithography and laser micromachining. Ablation opens replicated cavities, resulting in three-dimensional (3D) micropores. We previously demonstrated that microsieves can capture cells by passive pumping. Flow, capture yield, and cell survival depend on the control of the micropore geometry and must yield high reproducibility within the device and from device to device. We investigated the NOA81 film thickness, the laser pulse repetition rate, the number of pulses, and the beam focusing distance. For NOA81 films spin-coated between 600 and 1200 rpm, the pulse number controls the breaching of films to form the pore’s aperture and dominates the process. Pulse repetition rates between 50 and 200 Hz had no observable influence. We also explored laser focal plane to substrate distance to find the most effective ablation conditions. Scanning electron micrographs (SEM) of focused ion beam (FIB)-cut cross sections of the NOA81 micropores and inverted micropore copies in polydimethylsiloxane (PDMS) show a smooth surface topology with minimal debris. Our studies reveal that the combined process allows for a 3D micropore quality from device to device with a large enough process window for biological studies.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Sabahi-Kaviani

Lüttge

2020

Micromachines

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“…180 Moonen et al investigated capillary-based passive pumping for optimized neuronal cell trapping across a microsieve with gentle velocity proles and high survival rates. 181 Reports on capillary-based passive pumping in microuidics are summarised in Table 4.…”

Section: Capillarymentioning

confidence: 99%

Advances in passively driven microfluidics and lab-on-chip devices: a comprehensive literature review and patent analysis

et al. 2020

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“…Spatiotemporal investigations of electrophysiological function have also been performed on microelectrode arrays (MEAs) (Ban et al, 2007; van Vliet et al, 2007). We recently introduced organ-on-a-chip technology, which yields miniaturized systems that support two- and three-dimensional (2D and 3D) cell culture formats (Frimat et al, 2015; Bastiaens et al, 2018; Moonen et al, 2018; Xie et al, 2018). These on-chip low-volume culture systems can forward-engineer brain-like tissues as well as other organ features from a small number of human cells and simply rely on internal diffusion facilitated by the microfluidic approach (Ronaldson-Bouchard and Vunjak-Novakovic, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Need for Physiological Micro-Nanofluidic Systems of the Brain

Frimat

Lüttge

2019

Front. Bioeng. Biotechnol.

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In this article, we review brain-on-a-chip models and associated underlying technologies. Micro-nanofluidic systems of the brain can utilize the entire spectrum of organoid technology. Notably, there is an urgent clinical need for a physiologically relevant microfluidic platform that can mimic the brain. Brain diseases affect millions of people worldwide, and this number will grow as the size of elderly population increases, thus making brain disease a serious public health problem. Brain disease modeling typically involves the use of in vivo rodent models, which is time consuming, resource intensive, and arguably unethical because many animals are required for a single study. Moreover, rodent models may not accurately predict human diseases, leading to erroneous results, thus rendering animal models poor predictors of human responses to treatment. Various clinical researchers have highlighted this issue, showing that initial physiological descriptions of animal models rarely encompass all the desired human features, including how closely the model captures what is observed in patients. Consequently, such animal models only mimic certain disease aspects, and they are often inadequate for studying how a certain molecule affects various aspects of a disease. Thus, there is a great need for the development of the brain-on-a-chip technology based on which a human brain model can be engineered by assembling cell lines to generate an organ-level model. To produce such a brain-on-a-chip device, selection of appropriate cells lines is critical because brain tissue consists of many different neuronal subtypes, including a plethora of supporting glial cell types. Additionally, cellular network bio-architecture significantly varies throughout different brain regions, forming complex structures and circuitries; this needs to be accounted for in the chip design process. Compartmentalized microenvironments can also be designed within the microphysiological cell culture system to fulfill advanced requirements of a given application. On-chip integration methods have already enabled advances in Parkinson's disease, Alzheimer's disease, and epilepsy modeling, which are discussed herein. In conclusion, for the brain model to be functional, combining engineered microsystems with stem cell (hiPSC) technology is specifically beneficial because hiPSCs can contribute to the complexity of tissue architecture based on their level of differentiation and thereby, biology itself.

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Single cell trapping by capillary pumping using NOA81 replica moulded stencils

Cited by 15 publications

References 17 publications

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Advances in passively driven microfluidics and lab-on-chip devices: a comprehensive literature review and patent analysis

The Need for Physiological Micro-Nanofluidic Systems of the Brain

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