Single chain antibodies specific for fatty acids derived from a semi-synthetic phage display library

Novel phage display-derived mycolic acid-specific antibodies with potential for tuberculosis diagnosis

“…Earlier studies had already indicated the feasibility of isolating antibodies from phage display libraries against lipids (16)(17)(18). Using this methodology, we panned the Humanyx nonimmune Fab phage library against mycolic acid purifi ed from Mtb evaporated onto a solid surface.…”

“…A further complication is that traditional hybridoma methodologies typically do not generate high affi nity antibodies against lipids due to their poor immunogenicity unless conjugated to a protein carrier that enables lipid-specifi c B-cells to obtain T-cell help ( 15 ). However, in vitro antibody selection techniques such as phage display have allowed the generation of antibodies against nonpolar lipids such as 15-ketocholestane, methyl palmitate, and 11-dehydrothromboxane (16)(17)(18). These antibodies are able to detect free insoluble lipid deposited by evaporation of the carrier organic solvent, thus avoiding the need for the lipid to be emulsifi ed in an aqueous environment.…”

“…This could be because its mycolic acids in terms of fi ne structure such as total number of carbon atoms and fatty acid chain length more closely resemble mycobacterial mycolic acids as compared with some other genera such as Nocardia and Corynebacteria , which have fewer carbon atoms and shorter chain length ( 9 ). A previous study indicated that a phage display-derived antibody against methyl palmitate, a nonpolar lipid, could distinguish between this lipid and others with similar structure but longer carbon aliphatic tail ( 18 ). Therefore, while it is generally assumed that antibodies principally target and recognize polar epitopes even on lipids, it is possible that our antibodies can differentiate apolar portions of the mycolic acid molecule as well, such as the fatty acid tail.…”

The Phage Display Technique: Advantages and Recent Patents

“…Av. Antônio Carlos 6627, Pampulha 31.270-901, Belo Horizonte, Minas Gerais, Brazil; Tel: + 00 55 31 3409-2610; Fax: +00 55 31 3409-2610; E-mail: vasco@icb.ufmg.br antibodies [9], or, non-protein molecules including fatty acids [10], polysaccharides [10,11], phospholipids [12,13], RNAs [14,15] and DNAs [16,17], and, multimolecular complexes, such as viruses [18], cells [19,20], tissues and organs [21][22][23].…”

Considerations on Antibody-Phage Display Methodology

“…Aside from that a couple of single chain Fv fragments have been discribed directed against human α-Synuclein with the therapeutic potential for treatment of Parkinson´s disease [109], human IL-1R [110], S1 protein from SARS [111], alpha helical region of acylphosphatase [112], human CD43 [113], botulinum neurotoxin A [114], Amyloid-beta [115], angiopoietin-2 [116], heroin metabolite 6-monoacetylmorphine [117], vascular endothelial growth factor (VEGF) [118,119,120], Bcl-2 [121], human IL-6 [122], Glycoprotein VI [123], human cytomegalovirus glycoproteins gB and gH [124], fatty acids [125] and Huntingtin aggregation [126,127]. establishment and growth of tumors in a more indirect way [145,146].…”