Single Chain Cationic Polymer Dot as a Fluorescent Probe for Cell Imaging and Selective Determination of Hepatocellular Carcinoma Cells

Özenler, Sezer; Yucel, Muge; Tüncel, Özge; Kaya, Hakan; Özçelik, Serdar; Yıldız, Ümit Hakan

doi:10.1021/acs.analchem.9b02300

Cited by 12 publications

(14 citation statements)

References 25 publications

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“…In a related example, cationic single-chain Pdots were found to selectively accumulate in the nuclei of liver cancer cells but not in the nuclei of healthy liver cells. This effect enabled discrimination between the two populations in coculture and was attributed to altered nuclear membrane structure and larger pore size in the cancer cells …”

Section: Cellular Tissue and In Vivo Imaging And Theranosticsmentioning

confidence: 99%

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

et al. 2021

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Research related to the development and application of luminescent nanoparticles (LNPs) for chemical and biological analysis and imaging is flourishing. Novel materials and new applications continue to be reported after two decades of research. This review provides a comprehensive and heuristic overview of this field. It is targeted to both newcomers and experts who are interested in a critical assessment of LNP materials, their properties, strengths and weaknesses, and prospective applications. Numerous LNP materials are cataloged by fundamental descriptions of their chemical identities and physical morphology, quantitative photoluminescence (PL) properties, PL mechanisms, and surface chemistry. These materials include various semiconductor quantum dots, carbon nanotubes, graphene derivatives, carbon dots, nanodiamonds, luminescent metal nanoclusters, lanthanidedoped upconversion nanoparticles and downshifting nanoparticles, triplet−triplet annihilation nanoparticles, persistent-luminescence nanoparticles, conjugated polymer nanoparticles and semiconducting polymer dots, multi-nanoparticle assemblies, and doped and labeled nanoparticles, including but not limited to those based on polymers and silica. As an exercise in the critical assessment of LNP properties, these materials are ranked by several application-related functional criteria. Additional sections highlight recent examples of advances in chemical and biological analysis, point-of-care diagnostics, and cellular, tissue, and in vivo imaging and theranostics. These examples are drawn from the recent literature and organized by both LNP material and the particular properties that are leveraged to an advantage. Finally, a perspective on what comes next for the field is offered.

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Section: Cellular Tissue and In Vivo Imaging And Theranosticsmentioning

confidence: 99%

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

et al. 2021

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“…A variety of liquid biopsy methods for liver cancer biomarkers have been developed, including electrochemical, [12][13][14][15][16] acoustics, 17 and fluorescence detection. [18][19][20][21][22][23] Although electrochemical detection excels in vastly reducing ambient interferences, it involves complicated procedures and therefore remains difficult to implement clinically. Chemiluminescence or bioluminescence detection suffers from suboptimal signal intensities and signal-tonoise ratios, leading to impaired detection capabilities.…”

Section: Introductionmentioning

confidence: 99%

An Approach to the Simultaneous Detection of Multiple Biomarkers for the Early Diagnosis of Liver Cancer Using Quantum Dot Nanoprobes

Cheng

2022

Infectious Microbes and Diseases

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Biomarker-based early diagnosis of liver cancer is of high clinical value for reducing the mortality rate. However, it has been challenging to establish early detection methods with a single biomarker such as alpha-fetoprotein (AFP) because of limited diagnostic sensitivity and specificity. Therefore, developing multiplexed biomarker detection assays is crucially important for early diagnosis. Yet, simultaneous detection methods involving three or more biomarkers have been scarce. Here we suggest employing the serological biomarker panel of glypican-3 (GPC3), dickkopf-1 (DKK1), and AFP for liver cancer detection. We present a rapid simultaneous detection approach for the biomarker panel labeled with three fluorescent quantum dot nanoprobes (emission wavelengths at 565 nm, 605 nm, and 655 nm). As a proof-of-concept, simultaneous fluorescence detection of the biomarker panel was demonstrated using mixed reference samples containing human recombinant GPC3, DKK1, and AFP antigens. Our simultaneous detection approach conferred a linear range of 0.625-2.5 ng•mL À1 for the entire biomarker panel, which merits further clinical validation for the simultaneous and accurate determination of the biomarker panel in human serum samples.

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“…Single chain polymer dots in reduced diameter (<10 nm) have shown improved quantum yield, photostability, and colloidal stability [21][22][23][24][25][26][27][28] as compared to larger diameter polymer dots. Recently, Ozenler et al demonstrated the use of single-chain polymer dots for differentiation of cancer and healthy cells in co-culture medium [29]. Though single chain polymer dots are expected to show unambiguous advantages in imaging, post-functionalization possibilities are still limited due to the small surface area.…”

Section: Introductionmentioning

confidence: 99%

Gadolinium and Polythiophene Functionalized Polyurea Polymer Dots as Fluoro-Magnetic Nanoprobes

et al. 2022

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A rapid and one-pot synthesis of poly 3-thiopheneacetic acid (PTAA) functionalized polyurea polymer dots (Pdots) using polyethyleneimine and isophorone diisocyanate is reported. The one-pot mini-emulsion polymerization technique yielded Pdots with an average diameter of ~20 nm. The size, shape, and concentration of the surface functional groups could be controlled by altering the synthesis parameters such as ultrasonication time, concentration of the surfactant, and crosslinking agent, and the types of isocyanates utilized for the synthesis. Colloidal properties of Pdots were characterized using dynamic light scattering and zeta potential measurements. The spherical geometry of Pdots was confirmed by scanning electron microscopy. The Pdots were post-functionalized by 1,4,7,10 tetraazacyclododecane-1,4,7,10-tetraacetic acid for chelating gadolinium nanoparticles (Gd3+) that provide magnetic properties to the Pdots. Thus, the synthesized Pdots possess fluorescent and magnetic properties, imparted by PTAA and Gd3+, respectively. Fluorescence spectroscopy and microscopy revealed that the synthesized dual-functional Gd3+-Pdots exhibited detectable fluorescent signals even at lower concentrations. Magnetic levitation experiments indicated that the Gd3+-Pdots could be easily manipulated via an external magnetic field. These findings illustrate that the dua- functional Gd3+-Pdots could be potentially utilized as fluorescent reporters that can be magnetically manipulated for bioimaging applications.

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Single Chain Cationic Polymer Dot as a Fluorescent Probe for Cell Imaging and Selective Determination of Hepatocellular Carcinoma Cells

Cited by 12 publications

References 25 publications

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

An Approach to the Simultaneous Detection of Multiple Biomarkers for the Early Diagnosis of Liver Cancer Using Quantum Dot Nanoprobes

Gadolinium and Polythiophene Functionalized Polyurea Polymer Dots as Fluoro-Magnetic Nanoprobes

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